Fine Analysis of HIV-1-RNA Detection by in situ Hybridization1

2015 ◽  
pp. 62-68
Author(s):  
J. G. Fournier ◽  
S. Prevot ◽  
J. Audouin ◽  
A. Le Tourneau ◽  
P. Lebon ◽  
...  
Keyword(s):  
Rna Detection ◽  
Hiv 1

Monitoring HIV-1 treatment in immune-cell subsets with ultrasensitive fluorescence-in-situ hybridisation

The Lancet ◽  
1999 ◽  
Vol 353 (9148) ◽  
pp. 211-212 ◽  
Author(s):  
Bruce K Patterson ◽  
Mary Ann Czerniewski ◽  
John Pottage ◽  
Michelle Agnoli ◽  
Harold Kessler ◽  
...  
Keyword(s):  
Immune Cell ◽  
In Situ Hybridisation ◽  
Fluorescence In Situ Hybridisation ◽  
Immune Cell Subsets ◽  
Hiv 1

scholarly journals Failure of Fourth-Generation Enzyme Immunoassay in HIV Screening and Plasma HIV-1 RNA Detection in Recent High-Risk Behavior

Intervirology ◽  
2014 ◽  
Vol 57 (1) ◽  
pp. 49-51 ◽  
Author(s):  
Sarah Maylin ◽  
Sébastien Fouéré ◽  
François Simon ◽  
Constance Delaugerre
Keyword(s):  
High Risk ◽  
Enzyme Immunoassay ◽  
Risk Behavior ◽  
Fourth Generation ◽  
Hiv Screening ◽  
Rna Detection ◽  
High Risk Behavior ◽  
Hiv 1

scholarly journals RNA Detection and Subtype C Assessment of HIV-1 in Infants with Diarrhea in Ethiopia

2009 ◽  
Vol 3 (1) ◽  
pp. 19-23
Author(s):  
Workenesh Ayele ◽  
Tsehai Assefa ◽  
Sileshi Lulseged ◽  
Belete Tegbaru ◽  
Hiwot Berhanu ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Subtype C ◽  
Resource Limited Settings ◽  
Rna Detection ◽  
Resource Limited ◽  
Maternal Hiv ◽  
Hiv 1 ◽  
Established Infection

In the absence of chemoprophylaxis, HIV-1 transmission occurs in 13-42% of infants born to HIV-1 positive mothers. All exposed infants acquire maternal HIV-1 antibodies that persist for up to 15 months, thereby hampering diagnosis. In resource limited settings, clinical symptoms are the indices of established infection against validated laboratorybased markers. Here we enrolled 1200 children hospitalized for diarrheal and other illnesses. 20-25% of those tested, aged 15 months or younger, were found to be HIV-1-seropositive. Where sufficient plasma was available, HIV-1 RNA detection was performed using a subtype-insensitive assay, with 71.1% of seropositive infants presenting with diarrhea showing positive. From sub-typing analysis, we identified that viruses of the C’ sub-cluster were predominated amongst infants. Although this study may overestimate the HIV-1 frequency through testing symptomatic infants, diarrhea can be seen as a useful marker indicating HIV-1 infection in infants less than 15 months old.

Rapid In Situ Detection of PCR-Amplified HIV-1 DNA

1992 ◽  
Vol 1 (1) ◽  
pp. 98-102
Author(s):  
Gerard J. Nuovo ◽  
Michele Margiotta ◽  
Phyllis MacConnell ◽  
Joanne Becker
Keyword(s):  
In Situ Detection ◽  
Hiv 1

In Situ Multiplexing to Identify, Quantify, and Phenotype the HIV-1/SIV Reservoir Within Lymphoid Tissue

2022 ◽  
pp. 277-290
Author(s):  
Kathleen Busman-Sahay ◽  
Michael D. Nekorchuk ◽  
Carly Elizabeth Starke ◽  
Chi Ngai Chan ◽  
Jacob D. Estes
Keyword(s):  
Lymphoid Tissue ◽  
Hiv 1

Predictive value of HIV-1 RNA detection in plasma by branched DNA assay during long-term zidovudine therapy

1996 ◽  
Vol 15 (8) ◽  
pp. 639-645
Author(s):  
L. Cotte ◽  
M. A. Trabaud ◽  
P. Rougier ◽  
F. Bailly ◽  
F. Chapuis ◽  
...  
Keyword(s):  
Predictive Value ◽  
Zidovudine Therapy ◽  
Rna Detection ◽  
Dna Assay ◽  
Branched Dna ◽  
Hiv 1

scholarly journals Reassessment of the Roles of Integrase and the Central DNA Flap in Human Immunodeficiency Virus Type 1 Nuclear Import

2002 ◽  
Vol 76 (23) ◽  
pp. 12087-12096 ◽  
Author(s):  
Jeffrey D. Dvorin ◽  
Peter Bell ◽  
Gerd G. Maul ◽  
Masahiro Yamashita ◽  
Michael Emerman ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
In Situ Hybridization ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nuclear Import ◽  
Immunodeficiency Virus ◽  
Central Polypurine Tract ◽  
Hiv 1

ABSTRACT Human immunodeficiency virus type 1 (HIV-1) can infect nondividing cells productively because the nuclear import of viral nucleic acids occurs in the absence of cell division. A number of viral factors that are present in HIV-1 preintegration complexes (PICs) have been assigned functions in nuclear import, including an essential valine at position 165 in integrase (IN-V165) and the central polypurine tract (cPPT). In this article, we report a comparison of the replication and infection characteristics of viruses with disruptions in the cPPT and IN-V165. We found that viruses with cPPT mutations still replicated productively in both dividing and nondividing cells, while viruses with a mutation at IN-V165 did not. Direct observation of the subcellular localization of HIV-1 cDNAs by fluorescence in situ hybridization revealed that cDNAs synthesized by both mutant viruses were readily detected in the nucleus. Thus, neither the cPPT nor the valine residue at position 165 of integrase is essential for the nuclear import of HIV-1 PICs.

Analysis of HIV-1 expression in vivo with in situ hybridization and the polymerase chain reaction

1992 ◽  
Vol 6 (3) ◽  
pp. 215-221 ◽  
Author(s):  
B. Delord ◽  
M. Ottmann ◽  
M.-H. Schrive ◽  
J.-M. Ragnaud ◽  
J.-M. Seigneurin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Hiv 1

Comparison of Techniques for HIV-1 RNA Detection and Quantitation in Cervicovaginal Secretions

2001 ◽  
Vol 26 (2) ◽  
pp. 170-175 ◽  
Author(s):  
Grace C. John ◽  
Haynes Sheppard ◽  
Dorothy Mbori-Ngacha ◽  
Ruth Nduati ◽  
David Maron ◽  
...  
Keyword(s):  
Rna Detection ◽  
Comparison Of Techniques ◽  
Hiv 1
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