Comparison of Surfactant Biosynthesis in Fetal Rat Alveolar Type II Cells Grown in a Chemically Defined Medium or in the Presence of Serum

2015 ◽  
pp. 288-290
Author(s):  
Ga�lie Rolland ◽  
Jacques Bourbon ◽  
C�cile Valenza ◽  
Marie-Elisabeth Dufour ◽  
L�a Marin ◽  
...  
Keyword(s):  
Defined Medium ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Chemically Defined Medium ◽  
Alveolar Type Ii Cells ◽  
Fetal Rat

Isolation of alveolar type II cells from fetal rat lung by differential adherence in monolayer culture

1988 ◽  
Vol 960 (3) ◽  
pp. 441-453 ◽  
Author(s):  
Joseph J. Batenburg ◽  
Caroline J.M. Otto-Verberne ◽  
Ank A.W. Ten Have-Opbroek ◽  
Wies Klazinga
Keyword(s):  
Monolayer Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Alveolar Type Ii Cells ◽  
Fetal Rat ◽  
Fetal Rat Lung

Whole cell potassium currents in fetal rat alveolar type II cells cultured on Matrigel matrix

1996 ◽  
Vol 270 (4) ◽  
pp. L577-L586 ◽  
Author(s):  
S. Liu ◽  
A. J. Mautone
Keyword(s):  
Patch Clamp ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Whole Cell ◽  
Fetal Rat ◽  
Matrigel Matrix ◽  
Cells Cultured ◽  
In Cells

Patch-clamp studies were performed on fetal rat alveolar type II cells isolated at 19 days of gestation and cultured on either plastic for 7 days or Matrigel matrix (40-50 microliters/cm2) for 10 days. Before study, cells cultured on Matrigel matrix were dissociated from alveolar-like structures with enzymes, replated, and washed with cold buffer at a constant flow rate to remove residual gel. This wash significantly improved obtaining of successful seals. Potassium current-voltage relationships and maximum whole cell K+ conductance (normalized to membrane capacitance) were significantly changed with time in cells cultured on plastic, but no significant change occurred in cells cultured on Matrigel matrix. Application of 20 mM tetraet hyl ammonium, 2mM 4-aminopyridine, and 5mM BaCl2 significantly inhibited K+ currents, showing differences in channel sensitivity to these agents and a voltage-dependent blockage between culture groups or with time in culture. To conclude, we have developed a new method by which epithelial cells cultured on Matrigel matrix can be successfully studied with the use of patch-clamp techniques. Furthermore, these studies show that fetal type II cells have voltage-activated K+ channels and that channel density and their sensitivity to channel blockers are modulated by the substratum on which the cells are cultured.

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