Surfactant and Non-Surfactant Phosphatidylcholine Biosynthesis in Cultivated Fetal Rat Alveolar Type II Cells: Effect of Growth Factors

2015 ◽  
pp. 104-108
Author(s):  
Jacques Bourbon ◽  
Edith Doucet ◽  
Michel Rieutort ◽  
L�a Marin
Keyword(s):  
Growth Factors ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Fetal Rat ◽  
Phosphatidylcholine Biosynthesis

Sulfation of extracellular matrices modifies responses of alveolar type II cells to fibroblast growth factors

1996 ◽  
Vol 271 (5) ◽  
pp. L688-L697 ◽  
Author(s):  
P. L. Sannes ◽  
J. Khosla ◽  
P. W. Cheng
Keyword(s):  
Growth Factors ◽  
Biological Significance ◽  
Alveolar Type ◽  
Type I ◽  
Type Ii Cells ◽  
Type Ii ◽  
Fibroblast Growth ◽  
Alveolar Type Ii Cells ◽  
Proliferation And Differentiation ◽  
Type Ii Cell

The pulmonary alveolar basement membrane (BM) associated with alveolar type II cells has been shown to be significantly less sulfated than that of type I cells. To examine the biological significance of this observation, we measured the incorporation of 5-bromodeoxyuridine (BrdU) as an indicator of DNA synthesis in isolated rat type II cells cultured for 72-120 h on substrata that were naturally sulfated, not sulfated, or chemically desulfated in serum-free, hormonally defined media, with and without selected growth factors. The percentage of cells incorporating BrdU was significantly elevated by desulfated chondroitin sulfate in the presence of fibroblast growth factor-2 (FGF-2 or basic FGF) and depressed by heparin in the presence of either FGF-1 or acidic FGF or FGF-2. This depressive effect was lost by removing sulfate from the heparin. Some responses were dependent on the period of time in culture and concentration and molecular weight of the substrata. These observations support the notion that sulfation per se of certain components of BM is a key determinant of type II cell responses to select growth factors that may define patterns of proliferation and differentiation.

Keratinocyte growth factor increases mRNAs for SP-A and SP-B in adult rat alveolar type II cells in culture

1995 ◽  
Vol 269 (3) ◽  
pp. L344-L350 ◽  
Author(s):  
K. Sugahara ◽  
J. S. Rubin ◽  
R. J. Mason ◽  
E. L. Aronsen ◽  
J. M. Shannon
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Primary Cultures ◽  
Surfactant Proteins ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Adult Rat

The production of pulmonary surfactant, a complex of phospholipids and lung-specific surfactant proteins, is a primary function of alveolar type II cells. Although previous studies have demonstrated a role for cell-extracellular matrix interactions and normal cell shape in the maintenance of differentiated function in primary cultures of adult rat type II cells, a positive role for growth factors in surfactant protein gene expression in isolated normal adult type II cells has not been reported. In the present study, we have examined the effects of a panel of hormones, growth factors, and cytokines on the expression of mRNAs for surfactant proteins A, B, and C (SP-A, SP-B, and SP-C). Our results show that keratinocyte growth factor (KGF) induced a two- to threefold increase in steady-state levels of mRNAs for SP-A and SP-B, but had no effect on or decreased SP-C mRNA. The increase in SP-A mRNA was accompanied by an increase in SP-A protein. The effects of KGF were both dose and time dependent, and they could be neutralized by a monoclonal antibody against KGF. The effects of KGF were mimicked by acidic fibroblast growth factor, which will bind the KGF receptor. We conclude that KGF can support differentiation of alveolar type II cells as well as act as a mitogen, thus suggesting an important role for KGF in maintenance of the alveolar epithelium.

Dipalmitoyl-Phosphatidylcholine Biosynthesis is Induced by Non-Injurious Mechanical Stretch in a Model of Alveolar Type II Cells

Lipids ◽  
2013 ◽  
Vol 48 (8) ◽  
pp. 827-838 ◽  
Author(s):  
Despoina Pantazi ◽  
Eirini Kitsiouli ◽  
Athanasios Karkabounas ◽  
Theoni Trangas ◽  
George Nakos ◽  
...  
Keyword(s):  
Mechanical Stretch ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Alveolar Type Ii Cells ◽  
Dipalmitoyl Phosphatidylcholine ◽  
Phosphatidylcholine Biosynthesis

Isolation of alveolar type II cells from fetal rat lung by differential adherence in monolayer culture

1988 ◽  
Vol 960 (3) ◽  
pp. 441-453 ◽  
Author(s):  
Joseph J. Batenburg ◽  
Caroline J.M. Otto-Verberne ◽  
Ank A.W. Ten Have-Opbroek ◽  
Wies Klazinga
Keyword(s):  
Monolayer Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Alveolar Type Ii Cells ◽  
Fetal Rat ◽  
Fetal Rat Lung
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