Pseudomonas aeruginosa Ribosomal Vaccine. A Comparison of the Immunogenicity of Vaccines from Two Different Serotypes1

2015 ◽  
pp. 193-202 ◽  
Author(s):  
Michael M. Lieberman
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ribosomal Vaccine

scholarly journals Evidence for the presence of lipopolysaccharide in a ribonuclease-sensitive ribosomal vaccine of Pseudomonas aeruginosa.

1981 ◽  
Vol 31 (3) ◽  
pp. 896-905 ◽  
Author(s):  
R Gonggrijp ◽  
M P Volleberg ◽  
P J Lemmens ◽  
C P van Boven
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ribosomal Vaccine

Opsonic activity of antisera to ribosomal vaccine fractions with live and formalinized Pseudomonas aeruginosa

1986 ◽  
Vol 32 (6) ◽  
pp. 531-533 ◽  
Author(s):  
Michael M. Lieberman ◽  
Robert C. Allen
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Polymorphonuclear Leukocytes ◽  
Protein S ◽  
Opsonic Activity ◽  
Bacterial Cell Surface ◽  
Ribosomal Vaccine

The opsonic capacity of antisera to Pseudomonas aeruginosa ribosomal vaccine fractions was determined by a chemiluminescent technique. Antiserum to a vaccine fraction ("peak A") containing lipopolysaccharide (antiserum A), and antiserum to a vaccine fraction ("peak B"), which did not contain detectable amounts of lipopolysaccharide (antiserum B), were used to opsonify live or formalin-treated bacteria. Polymorphonuclear leukocytes were then stimulated by the opsonified bacteria in the presence of the chemiluminigenic probe, luminol, resulting in the observed chemiluminescence. The data obtained indicated that the antisera had comparable opsonic activity with live (untreated) bacteria. However, antiserum B had far less opsonic activity than did antiserum A when formalinized bacteria were used. Owing to the effects of formaldehyde on protein, these results were interpreted as evidence to suggest that the opsonic activities of the two antisera are dependent on different antigens on the bacterial cell surface. Antiserum A activity is probably dependent on lipopolysaccharide to a great extent, whereas antiserum B activity is most likely dependent primarily on a protein(s).

scholarly journals Ribonuclease-sensitive ribosomal vaccine of Pseudomonas aeruginosa.

1980 ◽  
Vol 27 (1) ◽  
pp. 204-210 ◽  
Author(s):  
R Gonggrijp ◽  
W J Mullers ◽  
P J Lemmens ◽  
C P van Boven
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ribosomal Vaccine

scholarly journals Specificity of the protective response induced by the slime layer ofPseudomonas aeruginosa

1974 ◽  
Vol 73 (1) ◽  
pp. 75-84 ◽  
Author(s):  
A. Mates ◽  
P. Zand
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protective Response ◽  
Passive Protection ◽  
Vaccine Preparation ◽  
Slime Layer ◽  
Active Protection ◽  
Ribosomal Vaccine

SUMMARYActive protection againstPseudomonas aeruginosacould be induced in mice by immunization with either the phenol killed cells or the alcohol precipitated fraction of the slime layer, or the ribosomal vaccine preparation. Passive protection could also be induced by injecting into mice antisera prepared in rabbits against these bacteria. This protection was due to the production of antibodies in reaction to the slime layer; the absorption of these antibodies by the slime caused the loss of protection. The fact that mice were also protected by vaccination with strains other than those used for challenging was attributed to the presence of an antigenically similar slime. Passive protection towards a heterologous strain, even one with an antigenically similar slime layer, was dependent on the dose of the challenging injection.

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