Opsonic activity of antisera to ribosomal vaccine fractions with live and formalinized Pseudomonas aeruginosa

1986 ◽  
Vol 32 (6) ◽  
pp. 531-533 ◽  
Author(s):  
Michael M. Lieberman ◽  
Robert C. Allen
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Polymorphonuclear Leukocytes ◽  
Protein S ◽  
Opsonic Activity ◽  
Bacterial Cell Surface ◽  
Ribosomal Vaccine

The opsonic capacity of antisera to Pseudomonas aeruginosa ribosomal vaccine fractions was determined by a chemiluminescent technique. Antiserum to a vaccine fraction ("peak A") containing lipopolysaccharide (antiserum A), and antiserum to a vaccine fraction ("peak B"), which did not contain detectable amounts of lipopolysaccharide (antiserum B), were used to opsonify live or formalin-treated bacteria. Polymorphonuclear leukocytes were then stimulated by the opsonified bacteria in the presence of the chemiluminigenic probe, luminol, resulting in the observed chemiluminescence. The data obtained indicated that the antisera had comparable opsonic activity with live (untreated) bacteria. However, antiserum B had far less opsonic activity than did antiserum A when formalinized bacteria were used. Owing to the effects of formaldehyde on protein, these results were interpreted as evidence to suggest that the opsonic activities of the two antisera are dependent on different antigens on the bacterial cell surface. Antiserum A activity is probably dependent on lipopolysaccharide to a great extent, whereas antiserum B activity is most likely dependent primarily on a protein(s).

Glycogen and various other polysaccharides stimulate the formation of exolipase by Pseudomonas aeruginosa

1982 ◽  
Vol 28 (6) ◽  
pp. 636-642 ◽  
Author(s):  
Gabriele Schulte ◽  
Leuthold Bohne ◽  
Ulrich Winkler
Keyword(s):  
Energy Source ◽  
Pseudomonas Aeruginosa ◽  
Protein Synthesis ◽  
Cell Surface ◽  
Bacterial Cell ◽  
De Novo ◽  
Conditioned Media ◽  
Specific Interactions ◽  
Bacterial Cell Surface ◽  
De Novo Protein Synthesis

Glycogen enhances the formation of exolipase by Pseudomonas aeruginosa ATCC 9027 although the cells cannot utilize it as sole carbon and energy source. Glycogen is unable to influence exolipase activities in conditioned media after removal of the cells. Treatment of cells with glycogen does not promote overall protein synthesis but inhibitors of protein synthesis (e.g., rifampicin and chloramphenicol) prevent the glycogen effect, suggesting that specific de novo protein synthesis is required. In addition to glycogen, 5 other polysaccharides (among 13 tested) were found to have exolipase-enhancing ability. The results are discussed with regard to the detachment hypothesis of U. K. Winkler and M. Stuckmann (1979. J. Bacteriol. 138: 663–670). According to this hypothesis polysaccharides are assumed to dislocate cell-bound lipase to the medium via specific interactions with the bacterial cell surface.

scholarly journals De novo synthesis of D- and L-fucosamine containing disaccharides

2013 ◽  
Vol 9 ◽  
pp. 332-341 ◽  
Author(s):  
Daniele Leonori ◽  
Peter H Seeberger
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Surface ◽  
Bacterial Cell ◽  
De Novo ◽  
Building Blocks ◽  
Biological Evaluation ◽  
De Novo Synthesis ◽  
Natural Sources ◽  
Bacterial Cell Surface ◽  
Cell Surface Glycans

The availability of rare monosaccharides that cannot be isolated from natural sources is currently limiting the access to the synthesis and the biological evaluation of complex bacterial cell-surface glycans. Here, we report the synthesis of D- and L-fucosamine building blocks by a de novo approach from L- and D-Garner aldehydes. These differentially protected monosaccharide building blocks were utilized to prepare disaccharides present on the surface of Pseudomonas aeruginosa bacteria.

Fe(iii)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

2021 ◽  
Author(s):  
Lilin Feng ◽  
Liang Gao ◽  
Daniel F. Sauer ◽  
Yu Ji ◽  
Haiyang Cui ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Immobilization ◽  
E Coli ◽  
Bacterial Cell Surface ◽  
Reversible Method

A facile and reversible method to immobilize His6-tagged proteins on the E. coli cell surface through the formation of an Fe(iii)-complex.

scholarly journals Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy

The Analyst ◽  
2014 ◽  
Vol 139 (12) ◽  
pp. 3174-3178 ◽  
Author(s):  
Ian L. Gunsolus ◽  
Dehong Hu ◽  
Cosmin Mihai ◽  
Samuel E. Lohse ◽  
Chang-soo Lee ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Super Resolution ◽  
Bacterial Cell Surface

The Bacterial Cell Surface in Relation to the Environment

1984 ◽  
pp. 194-219
Author(s):  
Stephen M. Hammond ◽  
Peter A. Lambert ◽  
Andrew N. Rycroft
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Bacterial Cell Surface

scholarly journals Cover Feature: Bacterial Cell‐Surface Display of Semisynthetic Cyclic Peptides (ChemBioChem 1/2019)

ChemBioChem ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 4-4
Author(s):  
Shubhendu Palei ◽  
Kira S. Becher ◽  
Christian Nienberg ◽  
Joachim Jose ◽  
Henning D. Mootz
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cyclic Peptides ◽  
Surface Display ◽  
Cell Surface Display ◽  
Bacterial Cell Surface

scholarly journals Spin-Dependent Electron Transport through Bacterial Cell Surface Multiheme Electron Conduits

2019 ◽  
Author(s):  
Suryakant Mishra ◽  
Sahand Pirbadian ◽  
Amit Kumar Mondal ◽  
Moh El-Naggar ◽  
Ron Naaman
Keyword(s):  
Electron Transport ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Shewanella Oneidensis ◽  
Extracellular Electron Transfer ◽  
Long Distance ◽  
Force Microscopy ◽  
Bacterial Cell Surface ◽  
Redox Active ◽  
Gain Energy

Multiheme cytochromes, located on the bacterial cell surface, function as long-distance (> 10 nm) electron conduits linking intracellular reactions to external surfaces. This extracellular electron transfer process, which allows microorganisms to gain energy by respiring solid redox-active minerals, also facilitates the wiring of cells to electrodes. While recent studies suggested that a chiral induced spin selectivity effect is linked to efficient electron transmission through biomolecules, this phenomenon has not been investigated in the extracellular electron conduits. Using magnetic conductive probe atomic force microscopy, Hall voltage measurements, and spin-dependent electrochemistry of the decaheme cytochromes MtrF and OmcA from the metal-reducing bacterium <i>Shewanella oneidensis</i> MR-1, we show that electron transport through these extracellular conduits is spin-selective. Our study has implications for understanding how spin-dependent interactions and magnetic fields may control electron transport across biotic-abiotic interfaces in both natural and biotechnological systems.

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