Evidence for Regulatory Cell-to-Cell Communication with Gonadotrophs, Lactotrophs, and Folliculostellate Cells in Rat Pituitary Cell Aggregates

2015 ◽  
pp. 163-175
Author(s):  
Carl Denef ◽  
Myriam Baes ◽  
Peter Carmeliet ◽  
Wim Robberecht ◽  
Wilfried Allaerts
Keyword(s):  
Cell Communication ◽  
Pituitary Cell ◽  
Cell Aggregates ◽  
Regulatory Cell ◽  
Folliculostellate Cells ◽  
Rat Pituitary

Ultrastructural and Functional Characteristics of Rat Pituitary Cell Aggregates*

Endocrinology ◽  
1982 ◽  
Vol 110 (2) ◽  
pp. 513-523 ◽  
Author(s):  
BERNADETTE VAN DER SCHUEREN ◽  
CARL DENEF ◽  
JEAN-JACQUES CASSIMAN
Keyword(s):  
Pituitary Cell ◽  
Cell Aggregates ◽  
Functional Characteristics ◽  
Rat Pituitary

Interaction of LHRH with growth hormone-releasing factor-dependent and -independent postnatal development of somatotrophs in rat pituitary cell aggregates

1995 ◽  
Vol 14 (1) ◽  
pp. 91-100 ◽  
Author(s):  
A Van Bael ◽  
M Proesmans ◽  
D Tilemans ◽  
C Denef
Keyword(s):  
Growth Hormone ◽  
Mrna Level ◽  
Inhibitory Effect ◽  
Pituitary Cell ◽  
Cell Aggregates ◽  
Dependent Development ◽  
Rat Pituitary ◽  
Comparable Effect ◽  
Number Of Cells

ABSTRACT Addition of LHRH for 40 h to aggregate cell cultures of 14-day-old rat pituitary significantly decreased the number of [3H]thymidine ([3H]T)-incorporating cells which immunostained for GH protein as well as the number of [3H]T-labelled cells expressing GH mRNA detectable by in situ hybridization with a digoxigenin-labelled riboprobe. The effect at the level of GH protein was seen at a dose of 1 nm LHRH. However, the effect at the GH mRNA level required a higher dose of LHRH (10 nm) or a longer incubation time (64 h). Treatment of the cultures for 40 h with 0·1 nm GH-releasing factor (GRF) provoked a 54% increase in the number of [3H]T-labelled cells containing GH mRNA and a 30% increase in the number of cells immunostained for GH protein. The latter effects of GRF were completely blocked by simultaneous addition of LHRH (1 nm) to the cultures. In the absence of GRF, LHRH (1 nM) also had an inhibitory effect on the total number of cells containing GH mRNA and a comparable effect on the total number of cells stained for GH protein. The present data show that LHRH is capable of inhibiting the GRF-independent as well as the GRF-dependent development of somatotrophs in postnatal rat pituitary in culture.

Peptides co-released with luteinizing hormone by perifused pituitary cell aggregates

1988 ◽  
Vol 60 (2-3) ◽  
pp. 151-161 ◽  
Author(s):  
B. Sion ◽  
E. Chanat ◽  
J. Duval ◽  
M.L. Thieulant
Keyword(s):  
Luteinizing Hormone ◽  
Pituitary Cell ◽  
Cell Aggregates

Expression of Ptx1 in the adult rat pituitary glands and pituitary cell lines: hormone-secreting cells and folliculo-stellate cells

1999 ◽  
Vol 298 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Reiko Kurotani ◽  
Shigeyuki Tahara ◽  
Naoko Sanno ◽  
Akira Teramoto ◽  
Pamela L. Mellon ◽  
...  
Keyword(s):  
Cell Lines ◽  
Stellate Cells ◽  
Pituitary Cell ◽  
Adult Rat ◽  
Rat Pituitary ◽  
Pituitary Glands

A background sodium conductance is necessary for spontaneous depolarizations in rat pituitary cell line GH3

1994 ◽  
Vol 266 (3) ◽  
pp. C709-C719 ◽  
Author(s):  
S. M. Simasko
Keyword(s):  
Cell Line ◽  
Calcium Current ◽  
Membrane Conductance ◽  
Potassium Currents ◽  
Pituitary Cell ◽  
Calcium Currents ◽  
Gh3 Cells ◽  
Sodium Conductance ◽  
Rat Pituitary ◽  
Voltage Dependent

The role of Na+ in the expression of membrane potential activity in the clonal rat pituitary cell line GH3 was investigated using the perforated patch variation of patch-clamp electrophysiological techniques. It was found that replacing bath Na+ with choline, tris(hydroxymethyl)aminomethane (Tris), or N-methyl-D-glucamine (NMG) caused the cells to hyperpolarize 20-30 mV. Tetrodotoxin had no effect. The effects of the Na+ substitutes could not be explained by effects on potassium or calcium currents. Although all three Na+ substitutes suppressed voltage-dependent calcium current by 10-20%, block of voltage-dependent calcium current by nifedipine or Co2+ did not result in hyperpolarization of the cells. There was no effect of the Na+ substitutes on voltage-dependent potassium currents. In contrast, all three Na+ substitutes influenced calcium-activated potassium currents [IK(Ca)], but only at depolarized potentials. Choline consistently suppressed IK(Ca), whereas Tris and NMG either had no effect or slightly increased IK(Ca). These effects on IK(Ca) also cannot explain the hyperpolarization induced by removing bath Na+. Choline always hyperpolarized cells yet suppressed IK(Ca). Furthermore, removing bath Na+ caused an increase in cell input resistance, an observation consistent with the loss of a membrane conductance as the basis of the hyperpolarization. Direct measurement of background currents revealed a 12-pA inward current at -84 mV that was lost upon removing bath Na+. These results suggest that this background sodium conductance provides the depolarizing drive for GH3 cells to reach the threshold for firing calcium-dependent action potentials.

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