Expression of Ptx1 in the adult rat pituitary glands and pituitary cell lines: hormone-secreting cells and folliculo-stellate cells

1999 ◽  
Vol 298 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Reiko Kurotani ◽  
Shigeyuki Tahara ◽  
Naoko Sanno ◽  
Akira Teramoto ◽  
Pamela L. Mellon ◽  
...  
Keyword(s):  
Cell Lines ◽  
Stellate Cells ◽  
Pituitary Cell ◽  
Adult Rat ◽  
Rat Pituitary ◽  
Pituitary Glands

scholarly journals C-type natriuretic peptide (CNP) effects in anterior pituitary cell lines: evidence for homologous desensitisation of CNP-stimulated cGMP accumulation in alpha T3-1 gonadotroph-derived cells

2000 ◽  
Vol 166 (1) ◽  
pp. 195-203 ◽  
Author(s):  
RC Fowkes ◽  
W Forrest-Owen ◽  
CA McArdle
Keyword(s):  
Natriuretic Peptide ◽  
Cell Lines ◽  
Guanylyl Cyclase ◽  
Anterior Pituitary ◽  
Phorbol Esters ◽  
Cell Types ◽  
Pituitary Cell ◽  
Anterior Pituitary Cell ◽  
Peptide Family ◽  
Rat Pituitary

C-type natriuretic peptide (CNP), the third member of the natriuretic peptide family, has been found at its highest tissue concentrations in the anterior pituitary, where it is localised in gonadotrophs. Its specific guanylyl cyclase-containing receptor, GC-B, is also expressed on several anterior pituitary cell types, and CNP potently stimulates cGMP accumulation in rat pituitary cell cultures and pituitary cell lines. The mouse gonadotroph-derived alpha T3-1 cell line has been shown to express CNP as well as GC-B (but not GC-A) receptors, suggesting that CNP may well be an autocrine regulator of gonadotrophs. Comparing effects of three natriuretic peptides (atrial natriuretic peptide (ANP), B-type natriuretic peptide (BNP) and CNP) on cGMP accumulation in four pituitary cell lines (alpha T3-1, TtT-GF, AtT-20 and GH(3)) we find that CNP is most potent and effective in alpha T3-1 cells. In these cells, CNP-stimulated cGMP accumulation was found to desensitise during a 30 min exposure to CNP. Pretreatment with CNP for up to 6 h also caused a significant reduction in the ability of CNP to subsequently stimulate cGMP accumulation. This effect was receptor specific, because pretreatment with sodium nitroprusside (an activator of nitric oxide-sensitive guanylyl cyclase), or with ANP or BNP, did not cause desensitisation of CNP-stimulated cGMP accumulation. Protein kinase C activation with phorbol esters also inhibited CNP-stimulated cGMP accumulation and such inhibition was also seen in cells desensitised by pretreatment with CNP. Thus it appears that the endogenous GC-B receptors of alpha T3-1 cells are subject to both homologous and heterologous desensitisation, that the mechanisms underlying these forms of desensitisation are distinct, and that cGMP elevation alone is insufficient to desensitise GC-B receptors.

Pituitary Follicular Cells: Their Origin, Possible Function and Fate

1974 ◽  
Vol 32 ◽  
pp. 34-35
Author(s):  
E. Horvath ◽  
K. Kovacs ◽  
G. Penz ◽  
C. Ezrin
Keyword(s):  
Fine Structure ◽  
Secretory Granules ◽  
Follicular Cells ◽  
Human Pituitary ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Junctional Complexes

Follicular structures, in the rat pituitary, composed of cells joined by junctional complexes and possessing few organelles and few, if any, secretory granules, were first described by Farquhar in 1957. Cells of the same description have since been observed in several species including man. The importance of these cells, however, remains obscure. While studying human pituitary glands, we have observed wide variations in the fine structure of follicular cells which may lead to a better understanding of their morphogenesis and significance.

NT-3: an Additional Neurotrophin Expressed in the Adult Rat Pituitary

1999 ◽  
Vol 25 (3-4) ◽  
pp. 295-305 ◽  
Author(s):  
Marie-Claude Amoureux
Keyword(s):  
Adult Rat ◽  
Rat Pituitary
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