HPLC Analysis of Tissue Thiobarbituric Acid-Reactive Material during Endotoxic Shock in Rats

2015 ◽  
pp. 42-45 ◽  
Author(s):  
M. Maiorino ◽  
L. Bordi ◽  
G. Consales ◽  
F. Ursini ◽  
G. P. Novelli
Keyword(s):  
Hplc Analysis ◽  
Endotoxic Shock ◽  
Thiobarbituric Acid ◽  
Reactive Material

293 EFFECT OF GUAIAZULENE ON IN VITRO BOVINE EMBRYO PRODUCTION

2007 ◽  
Vol 19 (1) ◽  
pp. 262 ◽  
Author(s):  
I. Dimitriadis ◽  
E. A. Rekka ◽  
E. Vainas ◽  
G. S. Amiridis ◽  
C. A. Rekkas
Keyword(s):  
Lipid Peroxidation ◽  
Embryo Development ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Early Embryo ◽  
Bovine Embryo ◽  
Embryo Production ◽  
Reactive Material

The substrates used in in vitro embryo production (IVP) mimic the in vivo fluids in which oocytes mature, oocytes are fertilized, and the early embryos develop (follicular and oviductal fluid). It is well established that oxidative stress negatively affects in vitro culture (IVC) outcomes. Guaiazulene (G) is a component of chamomile species oil with known antioxidant properties. In the present study, all IVP media were modified by the addition of G solutions so that the former exhibited a total protection against induced lipid peroxidation (TPaLP) similar to that of the respective in vivo environment. The IVP outcomes were then compared between G-processed and control oocytes. Bovine preovulatory follicular (BF) and oviductal (BO) fluid samples were collected from 10 Holstein 4- to 5-year-old cows in estrus. TPaLP was assessed according to the samples' ability to inhibit rat hepatic microsomal lipid peroxidation, by determination of the 2-thiobarbituric acid reactive material. TPaLP (mean % � SEM) of the BF and BO were 70.63 � 10.03 and 16.33 � 4.33, respectively, whereas those of the IVP [in vitro-matured (IVM), in vitro-fertilized (IVF), and IVC] media were lower (17.94 � 1.66, -1.82 � 0.78, and 14.57 � 1.26, respectively). TPaLP of the 0.1 mM G-modified IVP medium increased to 67.2 � 5.85, 19.98 � 2.49, and 69.19 � 6.22, respectively. A total of 2041 class A oocytes were used. The proportion of cleavage, early embryo development (embryos with more than 4 cells), or both after IVP (18 h IVM–5% CO2 in air, and 18 h IVF, 48 h IVC–5% CO2, 10% O2, 85% N) in the presence of G (n = 1237) during each of the IVP phases or any possible combination of IVP phases was compared with the respective control (C, n = 804). Statistical analysis was performed by a chi-squared test; P < 0.05 was considered significant. G improved cleavage and embryo development rates when present during IVM (79.4 and 57.8% vs. 64.5 and 38.2% for C) or both IVM and IVC (78.0 and 60.7% vs. 57.8 and 36.5%, respectively). When present only during 18 h of IVF, G had no effect on embryo production. However, an increased embryo development rate resulted from the combined exposure to G during IVF and IVM (56.4 vs. 29.6%), during IVF and IVC (55.3 vs. 35.5%), or at all IVP phases (56.6 vs. 34.9%). The latter effect resembled the one obtained after G addition only to the IVC medium (62.5 vs. 39.7%, respectively). We concluded that the addition of G to IVP substrates, at concentrations that mimic the in vivo TPaLP conditions, could promote bovine IVP efficiency.

scholarly journals Hydroxyl-radical-induced iron-catalysed degradation of 2-deoxyribose. Quantitative determination of malondialdehyde

1988 ◽  
Vol 252 (3) ◽  
pp. 649-653 ◽  
Author(s):  
K H Cheeseman ◽  
A Beavis ◽  
H Esterbauer
Keyword(s):  
Quantitative Determination ◽  
Hydroxyl Radical ◽  
Hydroxyl Radicals ◽  
Degradation Product ◽  
Thiobarbituric Acid ◽  
Iron Ions ◽  
Reactive Material ◽  
Gamma Radiolysis ◽  
Deoxyribose Degradation

The degradation of 2-deoxyribose to thiobarbituric acid-reactive material was investigated with two hydroxyl-radical-generating systems: (i) a defined gamma-radiolysis method and (ii) incubation with FeSO4 in phosphate buffer. In each case the thiobarbituric acid-reactive material can be accounted for by malondialdehyde, as measured by an h.p.l.c. method for free malondialdehyde. In the radiolysis system there is a large post-irradiation increase in free malondialdehyde if iron ions are added to the samples. It is proposed that this is due to iron ions catalysing the formation of hydroxyl radicals from radiolytically generated H2O2 as well as stimulating the breakdown of an intermediate deoxyribose degradation product. A mechanism for the formation of malondialdehyde during deoxyribose degradation is proposed.

scholarly journals Cerebral Na+, K+-ATPase Activity during Exposure to and Recovery from Acute Ischemia

1982 ◽  
Vol 2 (4) ◽  
pp. 457-465 ◽  
Author(s):  
V. MacMillan
Keyword(s):  
Atpase Activity ◽  
Carotid Arteries ◽  
Normal Values ◽  
Membrane Lipids ◽  
Energy State ◽  
Thiobarbituric Acid ◽  
Water Soluble ◽  
Acute Ischemia ◽  
Reactive Material ◽  
Free Radical Lipid

This study documents the Na+, K+-ATPase activity as well as selected parameters of oxidative metabolism and electrophysiological function in rat brain exposed to ischemia produced by electrocautery of the vertebral arteries and reversible occlusion of the carotid arteries. During a 0.5-h ischemic exposure in which the electroencephalograph (EEG) was abolished and energy metabolism severely compromised the Na+, K+-ATPase showed a capability for enhanced activity (120–140% of control), On recirculation, the Na+, K+-ATPase activity showed a phasic pattern, which was characterized by normal values at 0.25–2 h, increased values (115–125% of control) at 3–24 h, and, finally, normal values at 72 h of recirculation, respectively. The maintenance of Na+, K+-ATPase integrity was correlated with a gradual return of EEG activity and virtually complete restitution of the cerebral energy state during the 72 h of recirculation. Measurements of thiobarbituric acid reactive material and water soluble antioxidant during ischemia and recirculation gave no evidence of the presence of significant free radical lipid peroxidation in this model. It is concluded that Na+, K+-ATPase and its associated membrane lipids are not irreversibly damaged by ischemia in which the tissue lactacidosis is limited to less than 20 μmol g−1.

scholarly journals Copper-phenanthroline-induced site-specific oxygen-radical damage to DNA. Detection of loosely bound trace copper in biological fluids

1984 ◽  
Vol 218 (3) ◽  
pp. 983-985 ◽  
Author(s):  
J M C Gutteridge
Keyword(s):  
Oxygen Radicals ◽  
Biological Fluids ◽  
Thiobarbituric Acid ◽  
Oxygen Radical ◽  
Body Fluids ◽  
Reducing Agent ◽  
Reactive Material ◽  
Site Specific ◽  
Radical Damage ◽  
Micromolar Range

Copper(II) ions, in the presence of 1,10-phenanthroline, O2 and a reducing agent, degrade DNA with the release of thiobarbituric-acid-reactive material. This reaction, dependent on the formation of oxygen radicals, was made the basis of a sensitive and specific assay for loosely bound copper in body fluids. When applied to certain extracellular fluids, trace amounts of copper could be detected in the lower micromolar range.

Lipid peroxidation in rheumatoid arthritis: Thiobarbituric acid-reactive material and catalytic iron salts in synovial fluid from rheumatoid patients

1984 ◽  
Vol 66 (6) ◽  
pp. 691-695 ◽  
Author(s):  
David Rowley ◽  
John M. C. Gutteridge ◽  
David Blake ◽  
Margaret Farrs ◽  
Barry Halliwell
Keyword(s):  
Rheumatoid Arthritis ◽  
Lipid Peroxidation ◽  
Synovial Fluid ◽  
Knee Joint ◽  
Thiobarbituric Acid ◽  
Radical Reactions ◽  
Local Inflammation ◽  
Reactive Material ◽  
Iron Salts

1. Thiobarbituric acid (TBA)-reactive material is present in serum and knee joint synovial fluid from rheumatoid patients, consistent with lipid peroxidation occurring in vivo. 2. The amount of TBA-reactive material in synovial fluid correlates with the concentration of iron salts present as determined by the bleomycin method, presumably because iron is an important catalyst of radical reactions in vivo. 3. There appear to be significant correlations between the contents of TBA-reactive material and bleomycindetectable iron in synovial fluid and the activity of rheumatoid arthritis as assessed with a clinical index of local inflammation and with various laboratory parameters.

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