Comparison and Potential of Hypo-Osmotic and Iso-Osmotic Erythrocyte Ghosts and Carrier Erythrocytes as Drug and Enzyme Carriers

2015 ◽  
pp. 15-24
Author(s):  
R. A. Chalmers
Keyword(s):  
Erythrocyte Ghosts ◽  
Carrier Erythrocytes

Preparation and In Vitro Evaluation of Resealed Erythrocytes as A New Trend in Treatment of Asthma

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Mohammed Ibrahim ◽  
Alaa Zaky ◽  
Mohsen Afouna ◽  
Ahmed Samy
Keyword(s):  
In Vitro Release ◽  
Human Erythrocytes ◽  
The Body ◽  
Blood Levels ◽  
Time Interval ◽  
Burst Release ◽  
Prolonged Release ◽  
Nocturnal Asthma ◽  
Carrier Erythrocytes

Carrier erythrocytes are emerging as one of the most promising biological drug delivery systems investigated in recent decades. Beside its biocompatibility, biodegradability and ability to circulate throughout the body, it has the ability to perform extended release system of the drug for a long period. The ultimate goal of this study is to introduce a new carrier system for Salbutamol, maintaining suitable blood levels for a long time, as atrial to resolve the problems of nocturnal asthma medication Therefore in this work we study the effect of time, temperature as well as concentration on the loading of salbutamol in human erythrocytes to be used as systemic sustained release delivery system for this drug. After the loading process is performed the carrier erythrocytes were physically and cellulary characterized. Also, the in vitro release of salbutamol from carrier erythrocytes was studied over time interval. From the results it was found that, human erythrocytes have been successfully loaded with salbutamol using endocytosis method either at 25 Co or at 37 Co . The highest loaded amount was 3.5 mg/ml and 6.5 mg/ml respectively. Moreover, the percent of cells recovery is 90.7± 1.64%. Hematological parameters and osmotic fragility behavior of salbutamol loaded erythrocytes were similar that of native erythrocytes. Scanning electron microscopy demonstrated that the salbutamol loaded cells has moderate change in the morphology. Salbutamol releasing from carrier cell was 43% after 36 hours in phosphate buffer saline. The releasing pattern of the drug from loaded erythrocytes showed initial burst release in the first hour followed by a very slow release, obeying zero order kinetics. It concluded that salbutamol is successfully entrapped into erythrocytes with acceptable loading parameters and moderate morphological changes, this suggesting that erythrocytes can be used as prolonged release carrier for salbutamol.

scholarly journals Hypoxanthine Production from Nucleosides in Erythrocyte Ghosts

1959 ◽  
Vol 234 (12) ◽  
pp. 3243-3244
Author(s):  
William L. McLellan ◽  
Fabian J. Lionetti
Keyword(s):  
Erythrocyte Ghosts

scholarly journals THE EFFECTS OF NUCLEOSIDES ON PHOSPHATE AND PENTOSE METABOLISM IN ERYTHROCYTE GHOSTS

1957 ◽  
Vol 229 (2) ◽  
pp. 817-824
Author(s):  
Fabian J. Lionetti ◽  
William L. McLellan ◽  
Burnham S. Walker
Keyword(s):  
Erythrocyte Ghosts ◽  
Pentose Metabolism
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