Immunocytochemical Studies on Peptidergic Innervation in the Gastrointestinal Wall of Diabetic Rats

Author(s):  
S. Kishimoto ◽  
S. Shimizu ◽  
M. Yamamoto ◽  
G. Kajiyama ◽  
A. Miyoshi ◽  
...  
Physiology ◽  
1991 ◽  
Vol 6 (1) ◽  
pp. 26-28 ◽  
Author(s):  
G Ju ◽  
S Liu ◽  
X Zhang

The anterior pituitary is regulated by humoral and probably also by neural factors. Immunocytochemical studies have demonstrated substance P-, calcitonin gene-related peptide-, and galanin-like immunoreactive nerve fibers in the pars distalis of several mammalian species, and synapses have been identified on gland cells.


Endocrinology ◽  
1984 ◽  
Vol 115 (6) ◽  
pp. 2071-2076 ◽  
Author(s):  
G. A. HEDGE ◽  
L. J. HUFFMAN ◽  
T. GRUNDITZ ◽  
F. SUNDLER

2008 ◽  
Vol 213 (2) ◽  
pp. 388-396 ◽  
Author(s):  
Yen-Yu Lin ◽  
To-Jung Tseng ◽  
Yu-Lin Hsieh ◽  
Kai-Ren Luo ◽  
Whei-Min Lin ◽  
...  

Author(s):  
Burton B. Silver ◽  
Ronald S. Nelson

Some investigators feel that insulin does not enter cells but exerts its influence in some manner on the cell surface. Ferritin labeling of insulin and insulin antibody was used to determine if binding sites of insulin to specific target organs could be seen with electron microscopy.Alloxanized rats were considered diabetic if blood sugar levels were in excess of 300 mg %. Test reagents included ferritin, ferritin labeled insulin, and ferritin labeled insulin antibody. Target organs examined were were diaphragm, kidney, gastrocnemius, fat pad, liver and anterior pituitary. Reagents were administered through the left common carotid. Survival time was at least one hour in test animals. Tissue incubation studies were also done in normal as well as diabetic rats. Specimens were fixed in gluteraldehyde and osmium followed by staining with lead and uranium salts. Some tissues were not stained.


Author(s):  
Tetsuaki Osafune ◽  
Shuji Sumida ◽  
Tomoko Ehara ◽  
Eiji Hase ◽  
Jerome A. Schiff

Changes in the morphology of pyrenoid and the distribution of RuBisCO in the chloroplast of Euglena gracilis were followed by immunoelectron microscopy during the cell cycle in a light (14 h)- dark (10 h) synchronized culture under photoautotrophic conditions. The imrnunoreactive proteins wereconcentrated in the pyrenoid, and less densely distributed in the stroma during the light period (growth phase, Fig. 1-2), but the pyrenoid disappeared during the dark period (division phase), and RuBisCO was dispersed throughout the stroma. Toward the end of the division phase, the pyrenoid began to form in the center of the stroma, and RuBisCO is again concentrated in that pyrenoid region. From a comparison of photosynthetic CO2-fixation with the total carboxylase activity of RuBisCO extracted from Euglena cells in the growth phase, it is suggested that the carboxylase in the pyrenoid functions in CO2-fixation in photosynthesis.


2001 ◽  
Vol 120 (5) ◽  
pp. A674-A674 ◽  
Author(s):  
A FUNAKOSHI ◽  
M ICHIKAWA ◽  
Y SATO ◽  
S KANAI ◽  
M OHTA ◽  
...  

2007 ◽  
Vol 177 (4S) ◽  
pp. 264-265
Author(s):  
Ling De Young ◽  
KokBin Lim ◽  
Jeffery Carson ◽  
Trustin Domes ◽  
Mussa Al-Numi ◽  
...  

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