scholarly journals Immunocytochemical studies of pancreatic acinar cells in normal and streptozotocin-induced diabetic rats

Diabetologia ◽  
1986 ◽  
Vol 29 (9) ◽  
pp. 655-660 ◽  
Author(s):  
S. Gr�goire ◽  
M. Bendayan
Keyword(s):  
Acinar Cells ◽  
Diabetic Rats ◽  
Pancreatic Acinar Cells ◽  
Immunocytochemical Studies

Manganese action on pancreatic protein synthesis in normal and diabetic rats

1983 ◽  
Vol 245 (5) ◽  
pp. G628-G634 ◽  
Author(s):  
M. Korc
Keyword(s):  
Acinar Cells ◽  
Diabetic Rats ◽  
Pancreatic Acinar Cells ◽  
Diabetic Rat ◽  
Pancreatic Acini ◽  
Significant Stimulation ◽  
Biphasic Effect ◽  
Normal Rat ◽  
Streptozotocin Induced Diabetes

The effects of manganese on [3H]phenylalanine incorporation into protein were studied in pancreatic acini prepared from normal and streptozotocin-induced diabetic rats. In the absence of added Ca2+ manganese exerted a biphasic effect on [3H]phenylalanine incorporation in both groups of acini. Significant stimulation occurred at 3 X 10(-5) M manganese. At higher concentrations manganese inhibited incorporation. The magnitude of stimulation was similar in all acini, whereas the magnitude of inhibition was greater in acini from normal rats. Addition of Ca2+ to incubation media abolished the stimulatory effect of manganese in normal rat acini and greatly enhanced it in diabetic rat acini, significant stimulation now occurring at 10(-5) M manganese. The magnitude of inhibition was again greater in acini from normal rats. Insulin in vivo partially reversed the diabetes-induced alterations in acinar cell responsiveness to manganese. The present findings suggest that streptozotocin-induced diabetes is associated with postreceptor alterations in the pancreatic acinar cells.

Giant Mitochondria in Pancreatic Acinar Cells of Alloxan-Induced Diabetic Rats

2009 ◽  
Vol 141 (4) ◽  
pp. 301
Author(s):  
T. Sano ◽  
K. Ozaki ◽  
T. Matsuura ◽  
I. Narama
Keyword(s):  
Acinar Cells ◽  
Diabetic Rats ◽  
Pancreatic Acinar Cells ◽  
Giant Mitochondria

Giant Mitochondria in Pancreatic Acinar Cells of Alloxan-Induced Diabetic Rats

2010 ◽  
Vol 38 (4) ◽  
pp. 658-665 ◽  
Author(s):  
Tomoya Sano ◽  
Kiyokazu Ozaki ◽  
Tetsuro Matsuura ◽  
Isao Narama
Keyword(s):  
Acinar Cells ◽  
Diabetic Rats ◽  
Pancreatic Acinar Cells ◽  
Giant Mitochondria

scholarly journals Ultrastructural localization of GP2 in acinar cells of pancreas: presence of GP2 in endocytic and exocytic compartments.

1991 ◽  
Vol 39 (5) ◽  
pp. 575-588 ◽  
Author(s):  
A R Beaudoin ◽  
P St-Jean ◽  
G Grondin
Keyword(s):  
Acinar Cells ◽  
Ultrastructural Localization ◽  
Pancreatic Acinar Cells ◽  
Fibrillar Structure ◽  
Secretory Product ◽  
Immunocytochemical Localization ◽  
Tubular Structures ◽  
Zymogen Granules ◽  
The Matrix ◽  
Immunocytochemical Studies

GP2 is a glycoprotein found in pancreatic acinar cells. Its subcellular distribution suggests that it may be involved both in exocytosis and endocytosis. Immunocytochemical studies have demonstrated GP2 to be present on the membrane and in the matrix of zymogen granules, on Golgi saccules, on the apical and basolateral surfaces of the plasma membrane, and in the lumina of acini. In addition, this protein was observed in small vacuoles and tubular structures previously identified as "basal lysosomes," "snake-like tubules," and in lysosomes. Because the latter group of structures are involved in endocytosis, it is possible that GP2 may be involved in this phenomenon. GP2 was readily detectable in pancreatic juice and was totally sedimentable by ultracentrifugation, as assessed by Western blot analysis. Induced lysis of isolated zymogen granules also caused release of GP2 in a sedimentable form which, by electron microscopy, appeared as a fibrillar structure. Immunocytochemical localization of amylase was studied in parallel with GP2 and was found in the secretory product to be associated with thread-like structures, presumably the pancreatic thread protein. The physiological significance of these observations is discussed.

scholarly journals E4An Ultrastructural Study by Transmission Electron Microscope of Exocrine Pancreatic Cells in Diabetic Rats Treated with Herbal Combination

2019 ◽  
Vol 16 (4(Suppl.)) ◽  
pp. 0966
Author(s):  
Maysaa Adil Hadi
Keyword(s):  
Acinar Cells ◽  
Diabetic Rats ◽  
Degenerative Changes ◽  
Pancreatic Acinar Cells ◽  
Control Group ◽  
Cellular Activity ◽  
Pancreatic Acini ◽  
Group Iii ◽  
Transmission Electron ◽  
Ultrathin Sections

     The current study was designed to investigate the alterations in the ultrastructure of orgenelles and cellular activity of exocrine pancreatic acini of experimentally induced-diabetic rats and to assess the usefulness of herbal combination supplementation in improving the ultrastructure and cellular activity of exocrine pancreas. The number of albino male rats used were 24 which divided into equally 4 groups; group I: control group, group II: alloxan-induced diabetes mellitus (single intraperitoneal dose of alloxan 120 mg/kg for 3 days), group III: herbal combination treatment composed from the extracts of fenugreek seeds (Trigonella foenum-graecum), black cumin (Nigella sativa) seeds, rhizomes of ginger (Zingiber officinale), leaves of olive (Olea europeae), and seeds of ash (Fraxinus ssp). Each rat given 2.5 ml (0.5 ml from each five mixed plant extracts used) for 2 months. group IV: diabetes was induced as group II and treated with herbal combination as group III for 2 months. The processing for investigating by transmission electron microscopy were carried out for all pancreata taken from all groups. There was significant reduction (p<0.05) in mean of FBG levels in diabetic rats treated with herbal combination (group IV) as compared to diabetic control and could revert FBG to normal value compared to negative control The results of examination of semithin and ultrathin sections in diabetic rats revealed many degenerative changes in the exocrine pancreatic tissue in comparison to control group. These degenerative changes can be summarized as disturbances in the arrangement of pancreatic acinar cells and decreased secretory granules in addition to the vacuolation of cytoplasm and pyknotic nuclei were observed in semithin sections. Moreover, degenerated nuclei, vacuolation of cytoplasm, fragmentation of rough endoplasm reticulum and degranulation of the most pancreatic acinar cells were noticed in ultrathin sections. In contrast, the acinar cells of group administrated with herbal combination only had normal ultrastructure, cellular activity, and nuclei, well-developed rough endoplasmic reticulum with abundant zymogen granules. Also, most of the acinar cells retrieved their normal ultrastructure and cellular activity in diabetic group treated with herbal combination and herbal combination could decrease most of the degenerative changes caused by alloxan-induced diabetes. In conclusion, the daily supplementation with this herbal combination to diabetic rat for 2 months could achieving promising effects due to its cytoprotective influence and showed the ability to decreased degenerative damage in the ultrastructure and cellular activity of exocrine pancreatic acini in type 2 diabetic rats.

Altered intracellular Ca2+ regulation in pancreatic acinar cells from acute streptozotocin-induced diabetic rats

1996 ◽  
Vol 298 (3) ◽  
pp. 299-306 ◽  
Author(s):  
Takao Komabayashi ◽  
Hiroyuki Sawada ◽  
Tetsuya Izawa ◽  
Hiroshi Kogo
Keyword(s):  
Acinar Cells ◽  
Diabetic Rats ◽  
Pancreatic Acinar Cells

Visualization of CCK-evoked exocytic events in rat pancreatic acinar cells

2001 ◽  
Vol 120 (5) ◽  
pp. A24-A24
Author(s):  
H GAISANO ◽  
L TANG ◽  
L SHEU ◽  
W TRIMBLE
Keyword(s):  
Acinar Cells ◽  
Pancreatic Acinar Cells

Effects of oncogenic K-ras on pancreatic acinar cells In vitro

2001 ◽  
Vol 120 (5) ◽  
pp. A722-A722
Author(s):  
Y BI ◽  
C LOGSDON
Keyword(s):  
Acinar Cells ◽  
Pancreatic Acinar Cells
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