Polymorphonuclear Leukocyte (PMNL) Dependent and Independent Microcirculatory Plasma Leakage

2015 ◽  
pp. 86-99 ◽  
Author(s):  
C. Lundberg ◽  
J. Bj�rk ◽  
K. Lundberg ◽  
K. -E. Arfors
Keyword(s):  
Polymorphonuclear Leukocyte ◽  
Plasma Leakage

scholarly journals Differential inhibition of polymorphonuclear leukocyte recruitmentin vivoby dextran sulphate and fucoidan

1996 ◽  
Vol 5 (5) ◽  
pp. 346-357 ◽  
Author(s):  
N. Van Osselaer ◽  
M. Rampart ◽  
A. G. Herman
Keyword(s):  
Polymorphonuclear Leukocyte ◽  
Intradermal Injection ◽  
Initial Contact ◽  
Dextran Sulphate ◽  
Differential Inhibition ◽  
Rabbit Skin ◽  
Sulphated Polysaccharides ◽  
Independent Mechanism ◽  
Plasma Leakage

The selectin-mediated rolling of leukocytes along the endothelial cells is a prerequisite step followed by firm adhesion and extravasation into the inflamed tissue. This initial contact can be suppressed by sulphated polysaccharides. We have studied the effect of sulphated polysaccharides on the ultimate polymorphonuclear leukocyte (PMN) recruitment and plasma leakage in rabbit skin in response to intradermal injection of various inflammatory mediators. PMN infiltration evoked by various PMN chemoattractants (FMLP, C5a desArg, LTB4and IL-8) was significantly inhibited after intravenous injection of dextran sulphate (25 mg/kg), heparin (2 × 90 mg/kg) or fucoidan (1 mg/kg). PMN-dependent plasma leakage was equally well reduced by the different sulphated polymers. Vascular permeability induced by histamine or thrombin acting via a PMN-independent mechanism was not reduced. Fucoidan was the only polysaccharide able to suppress IL-1-induced PMN infiltration for 60–70%. Local administration of dextran sulphate had no effect on PMN-dependent plasma leakage. Differential inhibition of PMN recruitment was determined after injection of dextran sulphate or fucoidan depending on the type of insult. Therefore, these results suggest that different adhesion pathways are utilized during PMN recruitmentin vivoin response to chemoattractants and IL-1.

scholarly journals A monoclonal antibody to the membrane glycoprotein complex CD18 inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo

Blood ◽  
1987 ◽  
Vol 69 (1) ◽  
pp. 338-340 ◽  
Author(s):  
KE Arfors ◽  
C Lundberg ◽  
L Lindbom ◽  
K Lundberg ◽  
PG Beatty ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Polymorphonuclear Leukocyte ◽  
Critical Role ◽  
Skin Lesions ◽  
Membrane Glycoprotein ◽  
Glycoprotein Complex ◽  
Plasma Leakage ◽  
And Migration

Previous in vitro findings suggest a critical role for the polymorphonuclear leukocyte (PMN) membrane glycoprotein complex CD18 in PMN adherence and chemotaxis. We examined the effect of the murine monoclonal antibody (MoAb) 60.3, recognizing CD18, on induced PMN accumulation in vivo. Rabbits were pretreated with MoAb 60.3, and the chemotactic factors fMLP, leukotriene (LT)B4, and C5a, as well as histamine, were injected intradermally; 4 hours later, plasma leakage (125I-albumin) and the PMN accumulation (myeloperoxidase) were determined. Both PMN accumulation and PMN-dependent plasma leakage were abolished in the inflammatory skin lesions of rabbits pretreated with MoAb 60.3 as compared with control animals, whereas histamine-induced PMN-independent plasma leakage was unaffected. Intravital microscopy of the rabbit tenuissimus muscle revealed that MoAb 60.3 inhibited both PMN adherence in the venules and migration into the tissue following application of LTB4 and zymosan-activated serum (ZAS). Rolling of PMNs along the venular endothelium was unaffected. Thus, these experiments confirm and extend earlier in vitro findings of the critical role of the membrane glycoprotein complex, CD18, in PMN adherence and chemotaxis.

scholarly journals A monoclonal antibody to the membrane glycoprotein complex CD18 inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo

Blood ◽  
1987 ◽  
Vol 69 (1) ◽  
pp. 338-340 ◽  
Author(s):  
KE Arfors ◽  
C Lundberg ◽  
L Lindbom ◽  
K Lundberg ◽  
PG Beatty ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Polymorphonuclear Leukocyte ◽  
Critical Role ◽  
Skin Lesions ◽  
Membrane Glycoprotein ◽  
Glycoprotein Complex ◽  
Plasma Leakage ◽  
And Migration

Abstract Previous in vitro findings suggest a critical role for the polymorphonuclear leukocyte (PMN) membrane glycoprotein complex CD18 in PMN adherence and chemotaxis. We examined the effect of the murine monoclonal antibody (MoAb) 60.3, recognizing CD18, on induced PMN accumulation in vivo. Rabbits were pretreated with MoAb 60.3, and the chemotactic factors fMLP, leukotriene (LT)B4, and C5a, as well as histamine, were injected intradermally; 4 hours later, plasma leakage (125I-albumin) and the PMN accumulation (myeloperoxidase) were determined. Both PMN accumulation and PMN-dependent plasma leakage were abolished in the inflammatory skin lesions of rabbits pretreated with MoAb 60.3 as compared with control animals, whereas histamine-induced PMN-independent plasma leakage was unaffected. Intravital microscopy of the rabbit tenuissimus muscle revealed that MoAb 60.3 inhibited both PMN adherence in the venules and migration into the tissue following application of LTB4 and zymosan-activated serum (ZAS). Rolling of PMNs along the venular endothelium was unaffected. Thus, these experiments confirm and extend earlier in vitro findings of the critical role of the membrane glycoprotein complex, CD18, in PMN adherence and chemotaxis.

Platelet Metabolism during the Interiorization of Two Different Types of Particulate Matter

1972 ◽  
Vol 27 (02) ◽  
pp. 263-271 ◽  
Author(s):  
I. A. Cooper ◽  
P Cochrane ◽  
B. G. Firkin ◽  
K. J. Pinkard
Keyword(s):  
Particulate Matter ◽  
Polymorphonuclear Leukocyte ◽  
Anaerobic Metabolism ◽  
Human Platelets ◽  
Metabolic Changes ◽  
Polystyrene Latex ◽  
Considerable Time ◽  
Difference Of Opinion ◽  
Different Types ◽  
Aerobic And Anaerobic

SummaryIt has been suggested that human platelets possess the ability to phagocytose particulate matter similar to the polymorphonuclear leukocyte. However some difference of opinion has arisen regarding this contention, particularly as differences have been demonstrated with regard to the observed metabolic changes occurring in platelets related to such a process.The experiments reported in this paper were designed to observe the aerobic and anaerobic metabolism in human platelets during and following interiorization of two different particles, viz. polystyrene latex and thorotrast. The results of these experiments show a marked difference between both types of particles with regard to observable metabolic changes despite the rapid interiorization of both types of material. Some alteration occurs in both aerobic and anaerobic metabolism a considerable time after interiorization of latex, whereas no alteration could be demonstrated after interiorization of thorotrast. It is suggested that the interiorization of particulate matter is by some process other than phagocytosis and that observed metabolic changes related to latex may be due to a release reaction.

scholarly journals Hyperventilation in Children with Dengue Hemorrhagic Fever (DHF)

2019 ◽  
Vol 31 (9-10) ◽  
pp. 245-56
Author(s):  
Kasim Y. A. ◽  
Anky Tri Rini K. E. ◽  
Sumarmo S. P. S.
Keyword(s):  
Dengue Hemorrhagic Fever ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Lung Edema ◽  
Respiratory Problems ◽  
Ventilatory Pattern ◽  
Grade Ii ◽  
Plasma Leakage ◽  
The Difference ◽  
Respiratory Disturbance

Many studies of Dengue Hemorrhagic Fever (DHF) have been done but only a few revealed the respiratory status. Respiratory problems arise. because of plasma leakage through the 'damaged capillaries, causing lung edema and in turn result in hypoxemia. This later on will be compensated by a hyperventilation state. During a 6-month-period (May to September 1988), two aspects were studied in 85 patients hospitalized with DHF. First, the ventilatory pattern and second, the result of giving oxygen support in improving the respiratory disturbance, in this case alveolar hyperventilation. The incidence of alveolar hyperventilation in DHF grade II (DHF II) and Dengue Shock Syndrome (DSS) differed significantly. Hypoxemia occurred in DHF II and DSS with no significant differences. The difference of the incidence of metabolic acidosis in DHF II and DSS were significant. In DHF II patients having had hyperventilation state, oxygen therapy decreased respiration rate significantly and increased the PaC02 though not significantly.

ENDOTOXIN INDUCED PLASMA LEAKAGE WAS INHIBITED BY HYPERTONIC SALINE WITH OR WITHOUT DEXTRAN.

Shock ◽  
1998 ◽  
Vol 9 (Supplement) ◽  
pp. 27
Author(s):  
H. Carvalho-Gama ◽  
JA Matos ◽  
E Svensjö ◽  
E. Bouskela
Keyword(s):  
Hypertonic Saline ◽  
Plasma Leakage

Polymorphonuclear Leukocyte Function in Bacterial and Viral Infections

1982 ◽  
Vol 14 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Claus O. Solberg ◽  
Tore Kalager ◽  
Harry R. Hill ◽  
Johan Gleite
Keyword(s):  
Polymorphonuclear Leukocyte ◽  
Viral Infections ◽  
Leukocyte Function

scholarly journals Endothelial Cell Cortactin Phosphorylation by Src Contributes to Polymorphonuclear Leukocyte Transmigration In Vitro

2006 ◽  
Vol 98 (3) ◽  
pp. 394-402 ◽  
Author(s):  
Lin Yang ◽  
Jennifer R. Kowalski ◽  
Xi Zhan ◽  
Sheila M. Thomas ◽  
Francis W. Luscinskas
Keyword(s):  
Endothelial Cell ◽  
Polymorphonuclear Leukocyte ◽  
Leukocyte Transmigration
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