A Model of Isotypic Regulation in Antibody Production: the IgG2a Response to Sheep Erythrocytes

2015 ◽  
pp. 353-360
Author(s):  
Michel Seman
Keyword(s):  
Antibody Production ◽  
Sheep Erythrocytes ◽  
Igg2a Response

scholarly journals Immune complexes can trigger specific, T cell-dependent, autoanti-IgG antibody production in mice.

1985 ◽  
Vol 161 (1) ◽  
pp. 242-256 ◽  
Author(s):  
D A Nemazee
Keyword(s):  
Rheumatoid Factor ◽  
Antibody Production ◽  
Immune Complexes ◽  
B Lymphocyte ◽  
Active Form ◽  
Igg Antibody ◽  
Factor Production ◽  
Igg Isotypes ◽  
Igg2a Response

Immunization of mice with a combination of passively administered syngeneic IgG (anti-p-azophenylarsonate [anti-Ars]) antibody and a soluble, multivalent form of the antibody's corresponding antigen (Limulus polyphemus hemocyanin conjugated with Ars [Lph-Ars]) resulted in specific autoanti-IgG Fc (rheumatoid factor) production. The response was rapid and only anti-IgG of the IgM isotype is found. Because immunization with either the IgG antibody or the antigen alone did not result in rheumatoid antibody production, immune complexes appear to be the active form of the immunogens. Antibody/antigen ratios that resulted in maximal anti-IgG antibody responses were the same as those required for peak in vitro immunoprecipitation, i.e., equivalence. Previous exposure of the mice to the exogenously supplied antigen was not required for the response. The response to immune complexes is specific because mice immunized with IgG2a-containing complexes produced autoanti-IgG2a, while mice immunized with IgG1-containing complexes produced anti-IgG1 with little reactivity to other IgG isotypes. IgG2a blocked in its complement-fixing capacity was more effective in eliciting the anti-IgG2a response than native IgG2a, suggesting a possible role for the complement system in modulating the anti-IgG2a response. Induction of rheumatoid factor production by immune complexes could be induced in xid mice but not in nu/nu mice, indicating T lymphocyte dependence of the response. In contrast, the B lymphocyte activator lipopolysaccharide was able to elicit vigorous rheumatoid factor production in both nu/nu and normal mice, demonstrating that nu/nu mice contain B cells capable of making the response. Rheumatoid antibody produced in the immune complex- or LPS-induced responses is Fc specific and has relatively low affinity for IgG that is not bound to antigen.

scholarly journals ROLE OF COMPLEMENT IN INDUCTION OF ANTIBODY PRODUCTION IN VIVO

1974 ◽  
Vol 140 (1) ◽  
pp. 126-145 ◽  
Author(s):  
M. B. Pepys
Keyword(s):  
B Cells ◽  
Antibody Response ◽  
B Cell ◽  
Antibody Production ◽  
Antibody Responses ◽  
Cobra Venom ◽  
Sheep Erythrocytes ◽  
C3 Receptors

In an in vivo study in mice, suppression by the C3-cleaving protein of cobra venom (CoF), and other C3-reactive agents (zymosan, aggregated IgG, anti-C3 antibodies, and type III pneumococcal polysaccharide) of the thymus-dependent antibody responses to sheep erythrocytes, ovalbumin, and human IgG was demonstrated. The thymus-independent antibody response to polyvinyl-pyrrolidone was however unaffected by CoF. These and other published observations suggest that there may be a requirement for functional C3 in induction of thymus-dependent but not thymus-independent antibody production. A model for the role of C3 in lymphocyte cooperation is proposed based on these data analyzed in the light of existing knowledge of this process. It is postulated that fixed C3 interacting with macrophage See PDF for Structure and B-cell C3 receptors might enhance or facilitate T-dependent presentation of antigen to B cells.

scholarly journals Enhancement of antibody production against sheep erythrocytes using Theileria sergenti lysate antigen in different strains of inbred mice.

1986 ◽  
Vol 48 (3) ◽  
pp. 591-594 ◽  
Author(s):  
Shinya SHIMIZU ◽  
Takashi ONODERA ◽  
Tetsuro MINAMI ◽  
Yoshio TANAKA ◽  
Nobuo GOTO ◽  
...  
Keyword(s):  
Antibody Production ◽  
Inbred Mice ◽  
Sheep Erythrocytes ◽  
Different Strains

THE EFFECT OF CORTISONE ACETATE ON THE PRODUCTION OF CIRCULATING HEMOLYTIC ANTIBODIES IN THE MOUSE

1954 ◽  
Vol 32 (4) ◽  
pp. 372-382 ◽  
Author(s):  
Shirley E. Newsom ◽  
Marvin Darrach
Keyword(s):  
Antibody Production ◽  
Cortisone Acetate ◽  
Complete Inhibition ◽  
Experimental Conditions ◽  
Sheep Erythrocytes ◽  
Immunochemical Methods ◽  
Quantitative Procedure ◽  
Circulating Antibody

Quantitative immunochemical methods have been used to show that in the mouse cortisone acetate suppresses the formation of circulating hemolytic antibodies to sheep erythrocytes. The amount of suppression varies with the dose of both cortisone acetate and antigen. Experimental conditions are defined whereby an almost complete inhibition of circulating antibody occurs. The method serves as a quantitative procedure for comparing the effect of different steroids and hormones on antibody production as well as a means of studying possible cortisone antagonists.

THE EFFECT OF CORTISONE ACETATE ON THE PRODUCTION OF CIRCULATING HEMOLYTIC ANTIBODIES IN THE MOUSE

1954 ◽  
Vol 32 (1) ◽  
pp. 372-382 ◽  
Author(s):  
Shirley E. Newsom ◽  
Marvin Darrach
Keyword(s):  
Antibody Production ◽  
Cortisone Acetate ◽  
Complete Inhibition ◽  
Experimental Conditions ◽  
Sheep Erythrocytes ◽  
Immunochemical Methods ◽  
Quantitative Procedure ◽  
Circulating Antibody

Quantitative immunochemical methods have been used to show that in the mouse cortisone acetate suppresses the formation of circulating hemolytic antibodies to sheep erythrocytes. The amount of suppression varies with the dose of both cortisone acetate and antigen. Experimental conditions are defined whereby an almost complete inhibition of circulating antibody occurs. The method serves as a quantitative procedure for comparing the effect of different steroids and hormones on antibody production as well as a means of studying possible cortisone antagonists.

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