THE EFFECT OF CORTISONE ACETATE ON THE PRODUCTION OF CIRCULATING HEMOLYTIC ANTIBODIES IN THE MOUSE

1954 ◽  
Vol 32 (1) ◽  
pp. 372-382 ◽  
Author(s):  
Shirley E. Newsom ◽  
Marvin Darrach
Keyword(s):  
Antibody Production ◽  
Cortisone Acetate ◽  
Complete Inhibition ◽  
Experimental Conditions ◽  
Sheep Erythrocytes ◽  
Immunochemical Methods ◽  
Quantitative Procedure ◽  
Circulating Antibody

Quantitative immunochemical methods have been used to show that in the mouse cortisone acetate suppresses the formation of circulating hemolytic antibodies to sheep erythrocytes. The amount of suppression varies with the dose of both cortisone acetate and antigen. Experimental conditions are defined whereby an almost complete inhibition of circulating antibody occurs. The method serves as a quantitative procedure for comparing the effect of different steroids and hormones on antibody production as well as a means of studying possible cortisone antagonists.

THE EFFECT OF CORTISONE ACETATE ON THE PRODUCTION OF CIRCULATING HEMOLYTIC ANTIBODIES IN THE MOUSE

1954 ◽  
Vol 32 (4) ◽  
pp. 372-382 ◽  
Author(s):  
Shirley E. Newsom ◽  
Marvin Darrach
Keyword(s):  
Antibody Production ◽  
Cortisone Acetate ◽  
Complete Inhibition ◽  
Experimental Conditions ◽  
Sheep Erythrocytes ◽  
Immunochemical Methods ◽  
Quantitative Procedure ◽  
Circulating Antibody

Quantitative immunochemical methods have been used to show that in the mouse cortisone acetate suppresses the formation of circulating hemolytic antibodies to sheep erythrocytes. The amount of suppression varies with the dose of both cortisone acetate and antigen. Experimental conditions are defined whereby an almost complete inhibition of circulating antibody occurs. The method serves as a quantitative procedure for comparing the effect of different steroids and hormones on antibody production as well as a means of studying possible cortisone antagonists.

The effect of cortisone acetate treatment on the growth of Hymenolepis microstoma in mice

Parasitology ◽  
1972 ◽  
Vol 64 (2) ◽  
pp. 311-320 ◽  
Author(s):  
G. D. Moss
Keyword(s):  
Antibody Production ◽  
Technical Assistance ◽  
Cortisone Acetate ◽  
Circulating Antibody ◽  
Hymenolepis Microstoma

It has been demonstrated that Hymenolepis microstoma in mice treated with cortisone grow larger than those in control mice.The possibility of this being an immunosuppressive or growth-stimulating effect is discussed since the results from two strains of mice are different.Antibody production is suppressed in the cortisone-treated animals and it is suggested that the circulating antibody normally affects a partial rejection of the worm.I am grateful to Professor C. A. Hopkins and Dr T. S. C. Orr for many helpful comments during the course of this work and especially to Professor C. A. Hopkins for the facilities which he has placed at my disposal. Many thanks also to Miss Gillian Moore and Mr Jack Keys for their expert technical assistance. This work was in part supported by a grant from Fisons Pharmaceuticals Ltd.

scholarly journals THE MECHANISM OF ACTION OF CORTISONE IN EXPERIMENTAL HYPERSENSITIVITY

1953 ◽  
Vol 98 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Frederick G. Germuth
Keyword(s):  
Intravenous Injection ◽  
Mechanism Of Action ◽  
Antibody Production ◽  
Inhibitory Effect ◽  
Serum Sickness ◽  
Bovine Albumin ◽  
Renal Lesions ◽  
Granulomatous Lesions ◽  
Antigen Antibody ◽  
Circulating Antibody

Cortisone markedly suppressed the cardiovascular and renal lesions of serum sickness type hypersensitivity which ordinarily develop following the intravenous injection of bovine albumin. The inhibitory effect of cortisone on the allergic granulomatous lesions of the spleen was less striking; the lesions were less extensive, but the percentage of animals affected was unchanged. Cortisone in the dosage employed had no effect on the elimination of antigen following its intravenous administration or on the appearance of circulating antibody. These findings indicate that inhibition of the lesions of serum sickness by cortisone does not depend on the suppression of antibody production. Therefore, it is inferred that cortisone somehow protects the animal from the damaging effects of antigen-antibody union.

scholarly journals Immunologic Memory Of Phytohemagglutinin-Stimulated Lymphocytes

Blood ◽  
1969 ◽  
Vol 34 (6) ◽  
pp. 765-773 ◽  
Author(s):  
JEROME I. BRODY ◽  
HENRY D. SOLTYS
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Bacterial Adherence ◽  
Immunologic Memory ◽  
Experimental Conditions ◽  
Immune State ◽  
Lymphocyte Surface ◽  
Cellular Organelles

Abstract Using the technic of bacterial adherence, in which circumferential adhesion of microorganism to the lymphocyte surface indicates antibody production by this cell, phytohemagglutinin (PHA)-stimulated lymphocytes reacted differently towards E.coli, a natural, lifelong immunogen, and towards Salmonella, one which is acquired and overtly given when the need arises. Bacterial adherence after cell culture was markedly augmented in terms of numbers of participating organisms and cells when PHA-provoked lymphoblasts, rather than small, unchanged lymphocytes, were incubated with E.coli. In contrast, this type of response either did not occur at all or only in a limited, transient fashion when Salmonella was substituted as the laboratory antigen despite the fact that the PHA-exposed lymphocytes were obtained from donors immunized with Salmonella and had reacted actively with E.coli. This differential antigenic recognition by PHA-induced lymphoblasts in bacterial adherence supports the theory that PHA, under the experimental conditions outlined, acts on cellular organelles which govern the permanency of immunity and reflects differences between the natural and acquired immune state.

scholarly journals ROLE OF COMPLEMENT IN INDUCTION OF ANTIBODY PRODUCTION IN VIVO

1974 ◽  
Vol 140 (1) ◽  
pp. 126-145 ◽  
Author(s):  
M. B. Pepys
Keyword(s):  
B Cells ◽  
Antibody Response ◽  
B Cell ◽  
Antibody Production ◽  
Antibody Responses ◽  
Cobra Venom ◽  
Sheep Erythrocytes ◽  
C3 Receptors

In an in vivo study in mice, suppression by the C3-cleaving protein of cobra venom (CoF), and other C3-reactive agents (zymosan, aggregated IgG, anti-C3 antibodies, and type III pneumococcal polysaccharide) of the thymus-dependent antibody responses to sheep erythrocytes, ovalbumin, and human IgG was demonstrated. The thymus-independent antibody response to polyvinyl-pyrrolidone was however unaffected by CoF. These and other published observations suggest that there may be a requirement for functional C3 in induction of thymus-dependent but not thymus-independent antibody production. A model for the role of C3 in lymphocyte cooperation is proposed based on these data analyzed in the light of existing knowledge of this process. It is postulated that fixed C3 interacting with macrophage See PDF for Structure and B-cell C3 receptors might enhance or facilitate T-dependent presentation of antigen to B cells.

scholarly journals Comparison of techniques for detecting T-cell acute lymphocytic leukemia

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 210-215
Author(s):  
SL Melvin
Keyword(s):  
T Cell ◽  
Acute Lymphocytic Leukemia ◽  
Lymphocytic Leukemia ◽  
Membrane Receptors ◽  
Rosette Formation ◽  
Membrane Properties ◽  
Experimental Conditions ◽  
Sheep Erythrocytes ◽  
T Cell Antigens ◽  
Relationship Of

Three versions of the E-rosette test, one using untreated sheep erythrocytes at 37 degrees C, another using such cells at 4 degrees C, and a third using sheep erythrocytes treated with S-(2- aminoethyl)isothiouronium bromide hydrobromide (AET), were applied to each of 72 bone marrow specimens from as many unselected patients with untreated acute lymphocytic leukemia (ALL). The same specimens were also examined for T-cell antigens, based on reactivity with an antithymocyte serum. Lymphoblasts in eight ALL specimens formed E rosettes at 37 degrees C; no other E-positive specimens were identified when the assay was done at 4 degrees C. With AET-treated erythrocytes, lymphoblasts from these eight specimens and six additional specimens readily formed rosettes. T-cell antigens were detectable in all specimens positive for rosette formation withe untreated erythrocytes, in four of the six specimens positive for rosette formation with AET- treated erythrocytes, and in four specimens that showed no rosette formation under any of the experimental conditions used. Altogether, 18 specimens contained lymphoblasts with one or more surface markers characteristic of T-cell leukemia. These findings indicate that more specimens are likely to be identified at T-cell luekemias when E- rosette tests of increasing sensitivity and assayss for T-cell antigens are used. Some leukemic blasts do not possess the full array of membrane receptors and antigens usually associated with T cells. A combination of E-rosette tests and serologic tests is necessary to determine reliably the relationship of the test specimen to either T- cell ALL or common ALL and to establish the clinical significance of blasts that express membrane properties intermediate between those of T- cell ALL and common ALL.

A simulation program for quantitative procedure in EPMA

1996 ◽  
Vol 54 ◽  
pp. 480-481
Author(s):  
Claude Merlet
Keyword(s):  
Electron Probe ◽  
Quantitative Methods ◽  
Depth Distribution ◽  
Physical Parameters ◽  
Light Elements ◽  
Distribution Models ◽  
Experimental Conditions ◽  
X Ray ◽  
Gaussian Profile ◽  
Quantitative Procedure

In the last ten years the development of new X-ray depth distribution models in electron probe microanalysis (EPMA), has allowed to obtain accurate quantitative programs for stratified and massive samples. With these new quantitative methods, for the energy lines greater than 1keV and for massive samples, the results of the quantification are independent of the accelerating voltage (for an excited line). Nevertheless, for light elements, and for soft X-ray emission in complexe compounds, the choice of these optimum experimental conditions is not trivial. Moreover, the physical parameters and boundary conditions of the measurements are not well known.In an attempt to choose the optimum accelerating voltage, and to solve some difficulties in the quantification of low energy lines, a software (freeware) has been developed on PC and under the WINDOWS operating system. The calculation procedure is based upon the double partial gaussian profile ϕ(ρz) (recently developed for massive compounds). This description, flexible, precise and mathematically simple allows to compute rapidly the X-ray intensities. The program, which has been designed mainly for WDS electron probe, uses graphic simulations, and includes two sections:

scholarly journals Enhancement of antibody production against sheep erythrocytes using Theileria sergenti lysate antigen in different strains of inbred mice.

1986 ◽  
Vol 48 (3) ◽  
pp. 591-594 ◽  
Author(s):  
Shinya SHIMIZU ◽  
Takashi ONODERA ◽  
Tetsuro MINAMI ◽  
Yoshio TANAKA ◽  
Nobuo GOTO ◽  
...  
Keyword(s):  
Antibody Production ◽  
Inbred Mice ◽  
Sheep Erythrocytes ◽  
Different Strains
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