Ferritin Synthesis in Developing Erythroid Precursor Cells

2015 ◽  
pp. 81-85
Author(s):  
A. M. Konijn ◽  
C. Hershko ◽  
G. Izak
Keyword(s):  
Precursor Cells ◽  
Erythroid Precursor ◽  
Ferritin Synthesis ◽  
Ferritin Synthesis In

scholarly journals Ferroportin gene silencing induces iron retention and enhances ferritin synthesis in human macrophages

2004 ◽  
Vol 127 (5) ◽  
pp. 598-603 ◽  
Author(s):  
Anna Gallí ◽  
Gaetano Bergamaschi ◽  
Helios Recalde ◽  
Giorgio Biasiotto ◽  
Paolo Santambrogio ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Human Macrophages ◽  
Ferritin Synthesis ◽  
Iron Retention ◽  
Ferritin Synthesis In

scholarly journals Isolation of erythropoietin-sensitive cells from Friend virus-infected marrow cultures: characteristics of the erythropoietin response

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 751-758 ◽  
Author(s):  
M Bondurant ◽  
M Koury ◽  
SB Krantz ◽  
T Blevins ◽  
DT Duncan
Keyword(s):  
Terminal Differentiation ◽  
Erythroid Differentiation ◽  
Precursor Cells ◽  
Erythroid Cells ◽  
Friend Virus ◽  
Isolated Cells ◽  
Two Dimensional Gel Electrophoresis ◽  
Erythroid Precursor ◽  
Short Time

Abstract Murine erythroid precursor cells, stimulated to proliferate in vitro in the absence of added erythropoietin (EP) by the anemia strain of Friend virus (FVA), will subsequently respond to EP by complete erythrocyte differentiation. If not exposed to EP, the erythroid cells divide for about 120 hr in culture, and they maintain the potential for full differentiation in response to EP added at any time during the period from 72 to 120 hr. Between 96 and 120 hr of culture without added EP, the EP-sensitive erythroid precursor cells that have formed discrete erythroid bursts can be isolated in relatively large numbers from such cultures by plucking with a Pasteur pipette. The addition of EP initiates the final stages of erythroid differentiation, including heme synthesis in 70%-80% of these isolated cells. With respect to homogeneity of the precursor cells, quantity of EP-responsive cells obtainable, and uniformity of EP responsiveness, this system is uniquely favorable for biochemical studies of the late differentiation effects of EP. The overall changes in gene expression accompanying EP- induced terminal differentiation were examined by two-dimensional gel electrophoresis of proteins labeled for a short time with radioactive amino acids. Several new proteins are synthesized in these erythroid cells during terminal differentiation, but the number is a very small percentage of the total number of proteins being made. Thus, in this system, the effect of EP is to initiate expression of a small group of genes, including those for globins, spectrin, and other proteins involved in the final stages of erythroid differentiation.

scholarly journals The Effect of a Unique Region of Parvovirus B19 Capsid Protein VP1 on Endothelial Cells

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 606
Author(s):  
Ieva Rinkūnaitė ◽  
Egidijus Šimoliūnas ◽  
Daiva Bironaitė ◽  
Rasa Rutkienė ◽  
Virginija Bukelskienė ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Parvovirus B19 ◽  
Acute Infection ◽  
Endothelial Damage ◽  
Precursor Cells ◽  
Unique Region ◽  
Erythroid Precursor ◽  
Active Replication

Parvovirus B19 (B19V) is a widespread human pathogen possessing a high tropism for erythroid precursor cells. However, the persistence or active replication of B19V in endothelial cells (EC) has been detected in diverse human pathologies. The VP1 unique region (VP1u) of the viral capsid has been reported to act as a major determinant of viral tropism for erythroid precursor cells. Nevertheless, the interaction of VP1u with EC has not been studied. We demonstrate that recombinant VP1u is efficiently internalized by rats’ pulmonary trunk blood vessel-derived EC in vitro compared to the human umbilical vein EC line. The exposure to VP1u was not acutely cytotoxic to either human- or rat-derived ECs, but led to the upregulation of cellular stress signaling-related pathways. Our data suggest that high levels of circulating B19V during acute infection can cause endothelial damage, even without active replication or direct internalization into the cells.

scholarly journals Hemin-induced conversion of pyruvate kinase isozymes in K562 cells

Blood ◽  
1984 ◽  
Vol 64 (3) ◽  
pp. 754-757
Author(s):  
S Takegawa ◽  
T Shinohara ◽  
S Miwa
Keyword(s):  
Ion Exchange ◽  
Pyruvate Kinase ◽  
Early Stage ◽  
K562 Cells ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Precursor Cells ◽  
Electrophoretic Study ◽  
Hemoglobin F ◽  
Erythroid Precursor

The effects of hemin on the conversion of pyruvate kinase (PK) isozymes from the M2-type to the L-type in K562 cells were investigated. Immunofluorescence, ion exchange chromatography, and electrophoretic studies showed that the untreated K562 cells contained only the M2-type PK, while eight to 20 days after induction with hemin, concomitant with hemoglobin F synthesis, L-type PK levels increased while M2-type PK levels decreased. Electrophoretic study revealed three hybrid isozymes of the L-type and M2-type PK. We conclude that the conversion of PK isozymes from the M2-type to the L-type in erythroid precursor cells occurs in the early stage of maturation.

scholarly journals Induction of Ferritin Synthesis in Cells Infected with Mengo Virus

1996 ◽  
Vol 271 (16) ◽  
pp. 9851-9857 ◽  
Author(s):  
Michael R. Mulvey ◽  
Lukas C. Kühn ◽  
Douglas G. Scraba
Keyword(s):  
Ferritin Synthesis ◽  
Mengo Virus ◽  
In Cells ◽  
Ferritin Synthesis In

Induction of Ferritin Synthesis in vitro: Problems of Specificity Inherent in the Use of Iron Compounds

Enzyme ◽  
1990 ◽  
Vol 44 (1-4) ◽  
pp. 59-67 ◽  
Author(s):  
Jih-Jing Lin ◽  
William E. Walden ◽  
Robert E. Thach
Keyword(s):  
Iron Compounds ◽  
Ferritin Synthesis ◽  
Ferritin Synthesis In
Sign in / Sign up

Export Citation Format

Share Document