Induction of Ferritin Synthesis in vitro: Problems of Specificity Inherent in the Use of Iron Compounds

Enzyme ◽  
1990 ◽  
Vol 44 (1-4) ◽  
pp. 59-67 ◽  
Author(s):  
Jih-Jing Lin ◽  
William E. Walden ◽  
Robert E. Thach
Keyword(s):  
Iron Compounds ◽  
Ferritin Synthesis ◽  
Ferritin Synthesis In

scholarly journals Ferroportin gene silencing induces iron retention and enhances ferritin synthesis in human macrophages

2004 ◽  
Vol 127 (5) ◽  
pp. 598-603 ◽  
Author(s):  
Anna Gallí ◽  
Gaetano Bergamaschi ◽  
Helios Recalde ◽  
Giorgio Biasiotto ◽  
Paolo Santambrogio ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Human Macrophages ◽  
Ferritin Synthesis ◽  
Iron Retention ◽  
Ferritin Synthesis In

scholarly journals Induction of Ferritin Synthesis in Cells Infected with Mengo Virus

1996 ◽  
Vol 271 (16) ◽  
pp. 9851-9857 ◽  
Author(s):  
Michael R. Mulvey ◽  
Lukas C. Kühn ◽  
Douglas G. Scraba
Keyword(s):  
Ferritin Synthesis ◽  
Mengo Virus ◽  
In Cells ◽  
Ferritin Synthesis In

Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells

2020 ◽  
Vol 11 (12) ◽  
pp. 10907-10912
Author(s):  
Nathalia A. Gómez-Grimaldos ◽  
Leidy J. Gómez-Sampedro ◽  
José E. Zapata-Montoya ◽  
Gabriel López-García ◽  
Antonio Cilla ◽  
...  
Keyword(s):  
Degree Of Hydrolysis ◽  
Ferritin Synthesis ◽  
Bovine Plasma ◽  
Hydrolysis Of ◽  
Ferritin Synthesis In

Bovine plasma hydrolysates with a degree of hydrolysis of 19.1% have an iron chelating capacity of 38.5 ± 0.4% and increase the synthesis of ferritin in Caco-2 cells five-fold compared to the control.

Availability Of Iron

1970 ◽  
Vol 53 (4) ◽  
pp. 791-800
Author(s):  
Gwendolyn W Pla ◽  
James C Fritz
Keyword(s):  
United States ◽  
Iron Deficiency ◽  
Animal Studies ◽  
The United States ◽  
Deficiency Anemia ◽  
Iron Intake ◽  
Iron Compounds ◽  
Iron Supplements ◽  
High Incidence

Abstract The high incidence of iron deficiency anemia in the United States and elsewhere is caused by insufficient iron intake, plus poor utilization of many dietary sources of iron. There is need to select assimilable sources of iron for fortification of foods; animal studies show that many of the iron compounds used for cereal enrichment are poorly utilized. A method to measure availability of iron is proposed and is currently being studied collaboratively. Preliminary data indicate that in vitro solubility tests are not satisfactory to evaluate availability of iron supplements.

Sucrosomial Iron®: A New Highly Bioavaible Oral Iron Supplement

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4561-4561 ◽  
Author(s):  
Germano Tarantino ◽  
Elisa Brilli ◽  
Ylenia Zambito ◽  
Giulio Giordano ◽  
Francesco Equitani
Keyword(s):  
Iron Deficiency ◽  
Iron Uptake ◽  
Iron Bioavailability ◽  
Oral Iron ◽  
Deficiency Anemia ◽  
Sucrose Esters ◽  
Absorption Enhancers ◽  
Ferric Pyrophosphate ◽  
Ferritin Synthesis

Abstract Introduction: Iron deficiency is one of the most widespread nutritional deficiencies. Globally two billion people are suffering from iron- deficiency anemia (Hermida et al., 2010). Oral therapy for iron deficiency is mainly based on immediate release formulations of ferrous iron. However, modified formulations have been marketed to reduce gastrointestinal side effects and to prevent iron instability in the gastrointestinal tract. Overcoming biological barriers, including the gastrointestinal epithelial barriers, is a great challenge for pharmaceutical research and thus there is a need for new absorption enhancers with more favorable profile. Sucrose esters are widely used in the food industry, and there are reports on their potential use in pharmaceutical formulations as excipients (Szuts A et al., 2008). In vitro methods using cell cultures have been proposed to assess iron bioavailability as an alternative to in vivo methods. Caco-2 cells have shown numerous morphological and biochemical characteristics of enterocytes and have been successfully used to study iron absorption (Garcia et al., 1996; Jovani et al., 2001). Caco-2 monolayers formed a good barrier as reflected by high transepithelial resistance and positive immunostaining for junctional proteins. Sucrose esters in nontoxic concentrations significantly reduced resistance and impedance, and increased permeability of some components in Caco-2 monolayers. Recent data indicate that sucrose esters can enhance drug permeability through both the transcellular and paracellular routes (Kiss et al., 2014). Aim: The strong correlation between the published human absorption data and the iron uptake by Caco-2 cells makes them an ideal in vitro model to study iron bioavailability (Au and Reddy, 2000). For this, in the present study, we compared the bioavailability of innovative Oral Iron formulation based on Sucrosomial Iron¨ (Sideral¨) with three different Iron formulations (Figure 1). Materials and Methods: Sucrosomial Iron, preparation of ferric pyrophosphate convered by a phospholipids plus sucrose esters of fatty acids matrix; Lipofer¨, a water-dispersible micronised iron; Sunactive¨ ferric pyrophosphate, lecithin and emulsifiers. Results: The data showed that Sucrosomial Iron¨ (Sideral¨), is significantly more bioavaible than microencapsulated Ferric pyrophosphate ingredients, Lipofer¨ and Sunactive¨ and Ferrous Sulfate in Caco-2 cell model (Figure 1). Bibliography Au, A. P., Reddy, M. B. (2000). Caco-2 cells can be used to assess human iron bioavailability from a semipurified meal. J Nutr 130:1329-1334. Garcia et al. (1996). The Caco-2 cell culture system can be used as a model to study food iron availability. J Nutr 126:251-258. Hermida et al., Preparation and characterization of iron-containing liposomes: their effect on soluble iron uptake by Caco-2 cells Journal of Liposome Research, 2010, 1-10, Jovani et al. (2001) Calcium, iron, and zinc uptake from digests of infant formulas by Caco-2 cells. J Agric Food Chem 49:3480-3485. Kiss et al., (2014) Sucrose esters increase drug penetration, but do not inhibit p-glycoprotein in caco-2 intestinal epithelial cells J Pharm Sci. Oct;103(10):3107-19. Szuts A et al. (2008) Study of the effects of drugs on the structures of sucrose esters and the effects of solid-state interactions on drug release J Pharm Biomed Anal. 48: Figure 1. the graph shows the Ferritin levels of Caco-2 cells after iron formulations treatment. Sucrosomial Iron treated cells display significant increase of Ferritin synthesis compared to Lipofer and SunActive treated cells. Figure 1. the graph shows the Ferritin levels of Caco-2 cells after iron formulations treatment. Sucrosomial Iron treated cells display significant increase of Ferritin synthesis compared to Lipofer and SunActive treated cells. Disclosures Tarantino: Pharmanutra s.p.a.: Employment. Brilli:Pharmanutra s.p.a.: Employment.

Sign in / Sign up

Export Citation Format

Share Document