High Pressure Liquid Chromatographic Techniques for the Separation of Complex Mixtures of Occurring Porphyrins Naturally

2015 ◽  
pp. 465-471 ◽  
Author(s):  
R. E. Carlson ◽  
D. Dolphin
Keyword(s):  
High Pressure ◽  
Complex Mixtures ◽  
High Pressure Liquid Chromatographic ◽  
Chromatographic Techniques ◽  
Liquid Chromatographic

High Pressure Liquid Chromatographic Determination of Cholesterol in Foods

1981 ◽  
Vol 64 (1) ◽  
pp. 54-57 ◽  
Author(s):  
David R Newkirk ◽  
Alan J Sheppard
Keyword(s):  
High Pressure ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
High Pressure Liquid Chromatographic ◽  
Chromatographic Techniques ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic method that uses the benzoate ester of cholesterol has been developed for the measurement of cholesterol in foods. A 300 × 3.9 mm id μBondapak C18 column and a variable wavelength ultraviolet detector are used with a 100% methanol mobile phase. Amounts as low as 10 ng cholesterol benzoate can be detected. The results from the analyses of a variety of foods are given and compared with results from gas-liquid chromatographic analyses. The comparison of the 2 chromatographic techniques is favorable.

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