Beta-2-Microglobulin and HLA-Related Glycoproteins in Human Urine and Serum

2015 ◽  
pp. 66-88 ◽  
Author(s):  
Claude Vincent ◽  
Jean-Pierre Revillard
Keyword(s):  
Human Urine ◽  
Human Urine And Serum ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
Urine And Serum

Determination of beta 2-microglobulin in human urine and serum by latex immunoassay.

1981 ◽  
Vol 27 (6) ◽  
pp. 832-837 ◽  
Author(s):  
A M Bernard ◽  
A Vyskocil ◽  
R R Lauwerys
Keyword(s):  
Human Urine ◽  
Albumin Solution ◽  
Coated Particles ◽  
Analytical Recovery ◽  
Highly Sensitive ◽  
Human Urine And Serum ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
Urine And Serum

Abstract This highly sensitive method for determination of beta 2-microglobulin (beta 2-m) in human urine or serum is based on direct agglutination by beta 2-m of latex particles on which an antibody against beta 2-m is adsorbed. The agglutination is quantified by counting the remaining unagglutinated particles, or by turbidimetry. A novel aspect of this method is the capability to prevent nonspecific agglutination of the antibody-coated particles by diluting them with an albumin solution of well-defined characteristics (pH, freshness, concentration) just before the assay. The assayable concentration range is 1--32 micrograms/L, the detection limit 0.5 micrograms/L. Within-assay CV, based on 10 determinations of beta 2-m in urine and serum at two different dilutions, ranged from 4.6 to 8.7%. Between-assay CV, calculated from 10 determinations of beta 2-m in urine and serum, was 10 and 8.4%, respectively. Analytical recovery of beta 2-m in urine averaged 97% and in serum 104% (n = 10). No component of urine or serum interfered. Coefficients of correlation for beta 2-m in urine or serum as measured by radioimmunoassay and latex immunoassay were 0.97 and 0.93, respectively. Concentrations of beta 2-m in serum and urine from 33 healthy men (ages 20 to 67 years) averaged 1.5 mg/l and 54 micrograms/g of creatine, respectively.

Micellar‐Enhanced Spectrofluorimetric Determination of Trazodone Hydrochloride in Human Urine and Serum

2007 ◽  
Vol 40 (1) ◽  
pp. 151-162 ◽  
Author(s):  
Gong‐Jun Yang ◽  
Ping Liu ◽  
Xi‐Long Qu ◽  
Ming‐Shen ◽  
Chen‐Ying Wang ◽  
...  
Keyword(s):  
Human Urine ◽  
Spectrofluorimetric Determination ◽  
Human Urine And Serum ◽  
Urine And Serum ◽  
Trazodone Hydrochloride

scholarly journals Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence

2012 ◽  
Vol 58 (1) ◽  
pp. 88-94
Author(s):  
K. Li ◽  
L.Z. Nu ◽  
K.L. Khe ◽  
K.H. Song
Keyword(s):  
Human Urine ◽  
Pharmaceutical Preparations ◽  
Injection System ◽  
Chemiluminescence Method ◽  
Chemiluminescence Intensity ◽  
Complete Determination ◽  
Flow Injection System ◽  
Human Urine And Serum ◽  
Urine And Serum

A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescence intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng⋅ml-1 with the detection limit of 0.02 ng⋅ml-1 (3σ). At a flow rate of 2.0 ml⋅min-1, a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h-1 accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.

scholarly journals Twenty-Four Hour Human Urine and Serum Profiles of Bisphenol A during High-Dietary Exposure

2011 ◽  
Vol 123 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Justin G. Teeguarden ◽  
Antonia M. Calafat ◽  
Xiaoyum Ye ◽  
Daniel R. Doerge ◽  
Mona I. Churchwell ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Human Urine ◽  
Dietary Exposure ◽  
Human Urine And Serum ◽  
Urine And Serum

Fluorimetric determination of isatin in human urine and serum by liquid chromatography postcolumn photoirradiation

The Analyst ◽  
2001 ◽  
Vol 126 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Ken-ichi Mawatari ◽  
Makiko Segawa ◽  
Rumiko Masatsuka ◽  
Yasuko Hanawa ◽  
Fumio Iinuma ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Human Urine ◽  
Fluorimetric Determination ◽  
Human Urine And Serum ◽  
Urine And Serum

scholarly journals Heterogeneity of .BETA.2-microglobulin in human urine.

1979 ◽  
Vol 43 (8) ◽  
pp. 1707-1710 ◽  
Author(s):  
Nobuyasu TANAHASHI ◽  
Yutaka WATANABE ◽  
Fujizo YAMADA
Keyword(s):  
Human Urine ◽  
Beta 2 ◽  
Beta 2 Microglobulin
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