Interactions of Adenovirus Type 12 with Host Cell Chromosomes1

2015 ◽  
pp. 240-259 ◽  
Author(s):  
H. zur Hausen
Keyword(s):  
Host Cell ◽  
Adenovirus Type

Human adenovirus type 5 increases host cell fucosylation and modifies Ley antigen expression

Glycobiology ◽  
2019 ◽  
Vol 29 (6) ◽  
pp. 469-478
Author(s):  
Kathya Gutiérrez-Huante ◽  
Roberta Salinas-Marín ◽  
Héctor M Mora-Montes ◽  
Ramón A Gonzalez ◽  
Iván Martínez-Duncker
Keyword(s):  
Host Cell ◽  
Human Adenovirus ◽  
Antigen Expression ◽  
Adenovirus Type ◽  
Adenovirus Type 5 ◽  
Ley Antigen

scholarly journals Identification of Specific Cellular Genes Up-Regulated Late in Adenovirus Type 12 Infection

2005 ◽  
Vol 79 (4) ◽  
pp. 2404-2412 ◽  
Author(s):  
Andreas Dorn ◽  
Hongxing Zhao ◽  
Frederik Granberg ◽  
Marianna Hösel ◽  
Dennis Webb ◽  
...  
Keyword(s):  
Gene Expression ◽  
Host Cell ◽  
Late Phase ◽  
Viral Gene Expression ◽  
Adenovirus Type ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Viral Gene ◽  
Cell Functions ◽  
Cellular Genes

ABSTRACT The infection of human cells by adenoviruses leads to a gradual reduction in the activity of host cell functions while viral gene expression progresses in a regulated way. We used the DNA microarray technique to determine the transcriptional activity profiles of cellular genes upon infection with adenovirus type 12 (Ad12). The microarray data were validated by quantitative real-time PCR for genes which showed significant alterations after Ad12 infection. At 12 h postinfection, there is a striking up-regulation between 10- and 30-fold in the expression of the G1P2, IFIT1, and IFIT2 cellular immune response genes compared to mock-infected cells. At later stages of infection, when the majority of regulated cellular genes has been turned down, a limited number of cellular genes exhibit increased activities by factors of 3 or less. These genes belong to the signal transduction or transcriptional regulator classes or are active in protein degradation, like ANPEP, an aminopeptidase. The SCD and CYP2S1 genes function in lipid metabolism. The eucaryotic translation initiation factor 4 is up-regulated, and one of the major histocompatibility complex genes is diminished in activity. For two of the genes, one up-regulated (CTSF gene) and one down-regulated (CYR61 gene), alterations in gene activity were confirmed at the protein level by Western blotting experiments. Increased genetic activity of cellular genes late in adenovirus infection has not been reported previously and demonstrates that Ad12 has a sustained control of host cell gene expression well into the late phase of infection.

HYPERTHERMIA AND HOST-CELL REACTIVATION OF ADENOVIRUS 12

1978 ◽  
Vol 20 (1) ◽  
pp. 35-40 ◽  
Author(s):  
P. Lam ◽  
H. F. Stich
Keyword(s):  
Host Cell ◽  
Human Fibroblasts ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Populations ◽  
Hyperthermia Treatment ◽  
Cell Reactivation ◽  
Cultured Human Fibroblasts ◽  
Infected Cells ◽  
Host Cell Reactivation

Exposure of cultured human fibroblasts to hyperthermia delayed the host-cell reactivation of UV-irradiated human adenovirus type 12 (AD12). The experimental design consisted of irradiating human AD12 with UV doses ranging from 180 to 1800 ergs/mm2, infecting human cell populations at 37 °C, exposing the infected cells for 7 h to 39.5 °C and 41.8 °C, returning them to 37 °C and estimating the frequency of cells with intranuclear viral inclusion bodies (IB) 41 and 89 h after hyperthermia treatment. Hyperthermia reduced the fractions of fibroblasts with viral IB in the 41 h samples. By 89 h the capacity to form IB in the treated cells was comparable to that in control cells. Hyperthermia of 39.5 and 41.8 °C for 7 h did not affect the replication of nonirradiated AD12. The pattern of host-cell reactivation of AD12 following hyperthermia was compared to that in DNA repair deficient xeroderma pigmentosum cell populations.

scholarly journals The Replicative Capacities of Large E1B-Null Group A and Group C Adenoviruses Are Independent of Host Cell p53 Status

1999 ◽  
Vol 73 (3) ◽  
pp. 2074-2083 ◽  
Author(s):  
Andrew S. Turnell ◽  
Roger J. A. Grand ◽  
Phillip H. Gallimore
Keyword(s):  
Host Cell ◽  
Adenovirus Type ◽  
S Phase ◽  
Productive Infection ◽  
Replicative Capacity ◽  
Normal Human Skin ◽  
Viral Yield ◽  
P53 Status ◽  
In Cells

ABSTRACT Recent reports suggest that an early region 1B (E1B) 55,000-molecular-weight polypeptide (55K)-null adenovirus type 5 (Ad5) mutant (dl1520) can replicate to the same extent as wild-type (wt) Ad5 in cells either deficient or mutated in p53, implicating p53 in limiting viral replication in vivo. In contrast, we show here that the replicative capacity of Ad5 dl1520 is wholly independent of host cell p53 status, as is the replicative capacity of comparable Ad12 E1B 54K-null adenoviruses (Ad12dl620 and Ad12 hr703). Furthermore, we show that there is no requirement for complex formation between p53 and Ad5 E1B 55K or Ad12 E1B 54K for a productive infection, such that wt Ad5 and wt Ad12 will both replicate in cells which are null for p53. In addition, we find that these Ad5 and Ad12 mutant viruses induce S phase irrespective of the p53 status of the cell and that, therefore, S-phase induction does not correlate with the replicative capacity of the virus. Interestingly, the replicative capacities of the large E1B-null adenoviruses correlated positively with the ability to express E1B 19K and were related to the ability to repress premature adenovirus-induced apoptosis. Infection of primary human cells indicated that Ad5dl1520, wt Ad5, and wt Ad12 replicated better in cycling normal human skin fibroblasts (HSFs) than in quiescent HSFs. Thus, the cell cycle status of the host cell, upon infection, also influences viral yield.

scholarly journals Heparan Sulfate Glycosaminoglycans Are Involved in Adenovirus Type 5 and 2-Host Cell Interactions

Virology ◽  
2000 ◽  
Vol 268 (2) ◽  
pp. 382-390 ◽  
Author(s):  
Maria Cristina Dechecchi ◽  
Anna Tamanini ◽  
Alberto Bonizzato ◽  
Giulio Cabrini
Keyword(s):  
Heparan Sulfate ◽  
Host Cell ◽  
Adenovirus Type ◽  
Cell Interactions ◽  
Host Cell Interactions ◽  
Adenovirus Type 5

scholarly journals Cryo-EM structure of adenovirus type 3 fibre with desmoglein 2 shows a novel mode of receptor engagement

2018 ◽  
Author(s):  
Emilie Vassal-Stermann ◽  
Gregory Effantin ◽  
Chloe Zubieta ◽  
Wim Burmeister ◽  
Frédéric Iseni ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Host Cell ◽  
Adenoviral Vector ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Adenovirus Infection ◽  
Critical Step ◽  
Cryo Electron Microscopy ◽  
Type 3

AbstractAttachment of adenovirus (HAd) to host cell is a critical step of infection. This work reports the cryo-electron microscopy (cryo-EM) structure of a non-symmetrical complex smaller than 100kDa formed by the trimeric human adenovirus of type 3 fibre knob (HAd3K) and human desmoglein 2 (DSG2). The structure reveals a unique stoichiometry, shedding light to new adenovirus infection strategies and providing new insights for adenoviral vector development.

Purification and partial characterization of a 33 000 molecular weight endonuclease associated with human adenovirus type 5

1980 ◽  
Vol 26 (10) ◽  
pp. 1224-1231
Author(s):  
Linda W.-L. Tsang ◽  
R. G. Marusyk
Keyword(s):  
Molecular Weight ◽  
Infected Cell ◽  
Host Cell ◽  
Room Temperature ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Endonuclease Activity ◽  
Cell Extracts ◽  
Adenovirus Type 5

An endonuclease activity has been purified from human adenovirus type 5 (HAd5) virions and HAd5-infected cell extracts. The endonuclease activity is associated with a monomeric protein of molecular weight approximately 33 000. The endonuclease activity is more active at pH 4.5 than pH 7.2. Incubation of the enzyme at room temperature for periods of longer than 96 h results in a substantial increase in activity. The endonuclease activity is sensitive to EDTA at concentrations 10 mM or greater but is insensitive to 500 mM NaCl. Immunological analysis with endonuclease specific antiserum indicates that the endonuclease may be a host cell derived, viral modified, and incorporated protein.

scholarly journals SPOC1-Mediated Antiviral Host Cell Response Is Antagonized Early in Human Adenovirus Type 5 Infection

2013 ◽  
Vol 9 (11) ◽  
pp. e1003775 ◽  
Author(s):  
Sabrina Schreiner ◽  
Sarah Kinkley ◽  
Carolin Bürck ◽  
Andreas Mund ◽  
Peter Wimmer ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Response ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Host Cell Response ◽  
Adenovirus Type 5
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