scholarly journals Cryo-EM structure of adenovirus type 3 fibre with desmoglein 2 shows a novel mode of receptor engagement

2018 ◽  
Author(s):  
Emilie Vassal-Stermann ◽  
Gregory Effantin ◽  
Chloe Zubieta ◽  
Wim Burmeister ◽  
Frédéric Iseni ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Host Cell ◽  
Adenoviral Vector ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Adenovirus Infection ◽  
Critical Step ◽  
Cryo Electron Microscopy ◽  
Type 3

AbstractAttachment of adenovirus (HAd) to host cell is a critical step of infection. This work reports the cryo-electron microscopy (cryo-EM) structure of a non-symmetrical complex smaller than 100kDa formed by the trimeric human adenovirus of type 3 fibre knob (HAd3K) and human desmoglein 2 (DSG2). The structure reveals a unique stoichiometry, shedding light to new adenovirus infection strategies and providing new insights for adenoviral vector development.

The penton base of human adenovirus type 3 has the RGD motif

Gene ◽  
1994 ◽  
Vol 146 (2) ◽  
pp. 257-259 ◽  
Author(s):  
Alain Cuzange ◽  
Jadwiga Chroboczek ◽  
Bernard Jacrot
Keyword(s):  
Human Adenovirus ◽  
Adenovirus Type ◽  
Penton Base ◽  
Rgd Motif ◽  
Type 3

scholarly journals Sequence of the human adenovirus type 3 protease

1988 ◽  
Vol 16 (23) ◽  
pp. 11374-11374 ◽  
Author(s):  
Alain Houde ◽  
Joseph M. Weber
Keyword(s):  
Human Adenovirus ◽  
Adenovirus Type ◽  
Type 3

Construction and characterization of a replication-competent human adenovirus type 3-based vector as a live-vaccine candidate and a viral delivery vector

Vaccine ◽  
2009 ◽  
Vol 27 (8) ◽  
pp. 1145-1153 ◽  
Author(s):  
Qiwei Zhang ◽  
Xiaobo Su ◽  
Donald Seto ◽  
Bo-jian Zheng ◽  
Xingui Tian ◽  
...  
Keyword(s):  
Vaccine Candidate ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Live Vaccine ◽  
Delivery Vector ◽  
Type 3

Human adenovirus type 5 increases host cell fucosylation and modifies Ley antigen expression

Glycobiology ◽  
2019 ◽  
Vol 29 (6) ◽  
pp. 469-478
Author(s):  
Kathya Gutiérrez-Huante ◽  
Roberta Salinas-Marín ◽  
Héctor M Mora-Montes ◽  
Ramón A Gonzalez ◽  
Iván Martínez-Duncker
Keyword(s):  
Host Cell ◽  
Human Adenovirus ◽  
Antigen Expression ◽  
Adenovirus Type ◽  
Adenovirus Type 5 ◽  
Ley Antigen

scholarly journals The Adenovirus Dodecahedron: Beyond the Platonic Story

Viruses ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 718
Author(s):  
Solène Besson ◽  
Charles Vragniau ◽  
Emilie Vassal-Stermann ◽  
Marie Claire Dagher ◽  
Pascal Fender
Keyword(s):  
Electron Microscopy ◽  
Viral Entry ◽  
Physiological Role ◽  
Human Adenovirus ◽  
Structural Determinants ◽  
Cryo Electron Microscopy ◽  
Therapeutic Development ◽  
Adenovirus Receptor ◽  
Adenovirus Replication ◽  
Potential Use

Many geometric forms are found in nature, some of them adhering to mathematical laws or amazing aesthetic rules. One of the best-known examples in microbiology is the icosahedral shape of certain viruses with 20 triangular facets and 12 edges. What is less known, however, is that a complementary object displaying 12 faces and 20 edges called a ‘dodecahedron’ can be produced in huge amounts during certain adenovirus replication cycles. The decahedron was first described more than 50 years ago in the human adenovirus (HAdV3) viral cycle. Later on, the expression of this recombinant scaffold, combined with improvements in cryo-electron microscopy, made it possible to decipher the structural determinants underlying their architecture. Recently, this particle, which mimics viral entry, was used to fish the long elusive adenovirus receptor, desmoglein-2, which serves as a cellular docking for some adenovirus serotypes. This breakthrough enabled the understanding of the physiological role played by the dodecahedral particles, showing that icosahedral and dodecahedral particles live more than a simple platonic story. All these points are developed in this review, and the potential use of the dodecahedron in therapeutic development is discussed.

HYPERTHERMIA AND HOST-CELL REACTIVATION OF ADENOVIRUS 12

1978 ◽  
Vol 20 (1) ◽  
pp. 35-40 ◽  
Author(s):  
P. Lam ◽  
H. F. Stich
Keyword(s):  
Host Cell ◽  
Human Fibroblasts ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Populations ◽  
Hyperthermia Treatment ◽  
Cell Reactivation ◽  
Cultured Human Fibroblasts ◽  
Infected Cells ◽  
Host Cell Reactivation

Exposure of cultured human fibroblasts to hyperthermia delayed the host-cell reactivation of UV-irradiated human adenovirus type 12 (AD12). The experimental design consisted of irradiating human AD12 with UV doses ranging from 180 to 1800 ergs/mm2, infecting human cell populations at 37 °C, exposing the infected cells for 7 h to 39.5 °C and 41.8 °C, returning them to 37 °C and estimating the frequency of cells with intranuclear viral inclusion bodies (IB) 41 and 89 h after hyperthermia treatment. Hyperthermia reduced the fractions of fibroblasts with viral IB in the 41 h samples. By 89 h the capacity to form IB in the treated cells was comparable to that in control cells. Hyperthermia of 39.5 and 41.8 °C for 7 h did not affect the replication of nonirradiated AD12. The pattern of host-cell reactivation of AD12 following hyperthermia was compared to that in DNA repair deficient xeroderma pigmentosum cell populations.

scholarly journals iTRAQ-Based and Label-Free Proteomics Approaches for Studies of Human Adenovirus Infections

2013 ◽  
Vol 2013 ◽  
pp. 1-16 ◽  
Author(s):  
Hung V. Trinh ◽  
Jonas Grossmann ◽  
Peter Gehrig ◽  
Bernd Roschitzki ◽  
Ralph Schlapbach ◽  
...  
Keyword(s):  
A549 Cells ◽  
Correlation Coefficients ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Label Free ◽  
Absolute Quantitation ◽  
Software Packages ◽  
Itraq Label ◽  
Isobaric Tags ◽  
Type 3

Both isobaric tags for relative and absolute quantitation (iTRAQ) and label-free methods are widely used for quantitative proteomics. Here, we provide a detailed evaluation of these proteomics approaches based on large datasets from biological samples. iTRAQ-label-based and label-free quantitations were compared using protein lysate samples from noninfected human lung epithelial A549 cells and from cells infected for 24 h with human adenovirus type 3 or type 5. Either iTRAQ-label-based or label-free methods were used, and the resulting samples were analyzed by liquid chromatography (LC) and tandem mass spectrometry (MS/MS). To reduce a possible bias from quantitation software, we applied several software packages for each procedure. ProteinPilot and Scaffold Q+ software were used for iTRAQ-labeled samples, while Progenesis LC-MS and ProgenesisF-T2PQ/T3PQ were employed for label-free analyses. R2 correlation coefficients correlated well between two software packages applied to the same datasets with values between 0.48 and 0.78 for iTRAQ-label-based quantitations and 0.5 and 0.86 for label-free quantitations. Analyses of label-free samples showed higher levels of protein up- or downregulation in comparison to iTRAQ-labeled samples. The concentration differences were further evaluated by Western blotting for four downregulated proteins. These data suggested that the label-free method was more accurate than the iTRAQ method.

scholarly journals Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein

2021 ◽  
Vol 22 (23) ◽  
pp. 13034
Author(s):  
Søren Pfitzner ◽  
Jens B. Bosse ◽  
Helga Hofmann-Sieber ◽  
Felix Flomm ◽  
Rudolph Reimer ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Nuclear Envelope ◽  
Membrane Permeability ◽  
Nuclear Membrane ◽  
Membrane Damage ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Lamin A ◽  
Infected Cells ◽  
Adenovirus Type 5

The human adenovirus type 5 (HAdV5) infects epithelial cells of the upper and lower respiratory tract. The virus causes lysis of infected cells and thus enables spread of progeny virions to neighboring cells for the next round of infection. The mechanism of adenovirus virion egress across the nuclear barrier is not known. The human adenovirus death protein (ADP) facilitates the release of virions from infected cells and has been hypothesized to cause membrane damage. Here, we set out to answer whether ADP does indeed increase nuclear membrane damage. We analyzed the nuclear envelope morphology using a combination of fluorescence and state-of-the-art electron microscopy techniques, including serial block-face scanning electron microscopy and electron cryo-tomography of focused ion beam-milled cells. We report multiple destabilization phenotypes of the nuclear envelope in HAdV5 infection. These include reduction of lamin A/C at the nuclear envelope, large-scale membrane invaginations, alterations in double membrane separation distance and small-scale membrane protrusions. Additionally, we measured increased nuclear membrane permeability and detected nuclear envelope lesions under cryoconditions. Unexpectedly, and in contrast to previous hypotheses, ADP did not have an effect on lamin A/C reduction or nuclear permeability.

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