Effects of Steroid Hormones on Protein Synthesis in Uterine Epithelium and Stromal Tissue

2015 ◽  
pp. 221-226
Author(s):  
J. A. Smith ◽  
L. Martin ◽  
R. J. B. King
Keyword(s):  
Protein Synthesis ◽  
Steroid Hormones ◽  
Uterine Epithelium ◽  
Stromal Tissue

scholarly journals Protein Synthesis in Human Leukocytes and Lymphocytes: 1. Effect of Steroids and Sterols

Blood ◽  
1969 ◽  
Vol 34 (3) ◽  
pp. 348-356 ◽  
Author(s):  
SEYMOUR WERTHAMER ◽  
CARL HICKS ◽  
LEONARD AMARAL
Keyword(s):  
Protein Synthesis ◽  
Steroid Hormones ◽  
Plasma Protein ◽  
Human Lymphocytes ◽  
Amino Acid Incorporation ◽  
Binding Factor ◽  
In Vitro Effects ◽  
Physiologic Role

Abstract The in vitro effects of sterols, cholesterol and 3-methyl cholanthrene and steroids, cortisol, prednisolone and testosterone on protein synthesis in separate popultions of human lymphocytes and leukocytes has been investigated. It has been shown that all agents used result in the inhibition of protein synthesis under these conditions. It has also been shown that the inhibitory mechanism of the steroid hormones requires the presence of plasma, presumably as a protein binding factor in order to achieve its effect. The sterol, cholesterol and 3-methyl cholanthrene, in the absence of plasma, still inhibit amino acid incorporation. However, in the case of cholesterol, the magnitude of inhibition is lower than that observed in the presence of plasma, perhaps indicating a partial plasma dependence. The results presented therefore support the hypothesis that the inhibition of lymphocyte protein synthesis by steroid hormones occurs only when the steroid is bound to a plasma protein. The physiologic role of the plasma protein-cortisol complex and its relation to the condition of lymphopenia in man is discussed.

scholarly journals Expression of cellCAM-105 in the apical surface of rat uterine epithelium is controlled by ovarian steroid hormones

Reproduction ◽  
1990 ◽  
Vol 88 (1) ◽  
pp. 213-221 ◽  
Author(s):  
P. C. Svalander ◽  
P. Odin ◽  
B. O. Nilsson ◽  
B. Obrink
Keyword(s):  
Steroid Hormones ◽  
Uterine Epithelium ◽  
Apical Surface ◽  
Ovarian Steroid

scholarly journals Effects of testosterone on messenger ribonucleic acid and protein synthesis in rat seminal vesicle

1978 ◽  
Vol 174 (2) ◽  
pp. 543-551 ◽  
Author(s):  
Stephen J. Higgins ◽  
Joy M. Burchell
Keyword(s):  
Protein Synthesis ◽  
Seminal Vesicle ◽  
Steroid Hormones ◽  
Secretory Proteins ◽  
Total Acid ◽  
Free System ◽  
Messenger Ribonucleic Acid ◽  
Mrna Population ◽  
Rat Seminal Vesicle

In a previous report [Higgins et al. (1976) Biochem. J.158, 271–282] we described the effects of alterations in androgen status on the synthesis of two basic secretory proteins of the rat seminal vesicle. In the present paper we examine the effects of testosterone on the activity of mRNA in the seminal vesicle. Total cellular poly(A)-rich RNA was isolated and translated in a cell-free system prepared from wheat germ. Translation products were separated on denaturing polyacrylamide gels and the protein bands corresponding to the two basic secretory proteins were identified immunologically. Incorporation of radioactive methionine into these bands was taken as a measure of the individual mRNA activities. Total mRNA activity was estimated by radioactivity in total acid-precipitable material. The results show that 1 to 2 weeks after castration the activities of mRNA molecules for the basic secretory proteins were decreased 10–20-fold on a tissue basis. Testosterone given in vivo rapidly and substantially restores mRNA activity to normal. Since these changes correlate closely with variations in the rates of synthesis of the secretory proteins in whole cells it suggests that androgenic steroids control protein synthesis chiefly via mRNA availability. In this respect their action resembles those of other steroid hormones acting in other systems. However, these effects of testosterone on the mRNA molecules for the major secretory proteins could not be distinguished from those on total mRNA. Thus the proportion of the total mRNA population accounted for by the two specific mRNA molecules showed less than a 2-fold variation with androgen status. Similarly the two secretory proteins always accounted for 25–33% of general protein synthesis. This is in sharp contrast with the markedly differential effects of other steroid hormones controlling synthesis of major proteins in other well-studied systems. We interpret our results as indicating that testosterone regulates the mRNA population of the seminal vesicle as a whole.

THE DEVELOPMENT OF AN IN VITRO SYSTEM FOR ESTIMATING OXYTOCINASE IN THE RABBIT HYPOTHALAMUS AND THE EFFECTS OF SOME PROTEIN SYNTHESIS INHIBITORS AND OESTRADIOL-17β ON ENZYME ACTIVITY

1974 ◽  
Vol 75 (3) ◽  
pp. 443-451 ◽  
Author(s):  
Dona A. Frith ◽  
K. C. Hooper
Keyword(s):  
Protein Synthesis ◽  
Enzyme Activity ◽  
Steroid Hormones ◽  
Actinomycin D ◽  
Protein Synthesis Inhibitors ◽  
In Vitro System

ABSTRACT An in vitro system for investigating the effects of steroid hormones and protein synthesis inhibitors on hypothalamic peptidases inactivating oxytocin has been developed. In the presence of oestradiol-17β enzyme activity was increased in the in vitro system whilst this increase was blocked completely by cycloheximide and partially blocked by actinomycin-D. It is apparent therefore that oestradiol-17β acts directly on the hypothalamus stimulating oxytocinase activity.

scholarly journals The Effect of Different Hormones and Antibiotics on Activity of AST Enzyme and its Isozymes in Wistar Rats

2018 ◽  
Vol 46 (1) ◽  
pp. 8
Author(s):  
Minoo Azani ◽  
Asghar Moshtaghie ◽  
Ali Asghar Rastegari
Keyword(s):  
Protein Synthesis ◽  
Steroid Hormones ◽  
Aspartate Aminotransferase ◽  
Wistar Rats ◽  
Sesame Oil ◽  
Control Group ◽  
Short Term ◽  
Group 2 ◽  
Tetracycline Group

Background: One of the valuable tests for diagnosis of cardiovascular and liver diseases is measuring of AST activity. One of the main enzymes of transaminases group is aspartate aminotransferase. Previous Studies have shown that some alteration may occur in mitochondria function as the result of different disease or taking different medication; these changes in mitochondrial and cytosolic AST isozymes can be the sign of disorders. According to the role of steroid hormone in induction of its effects on protein synthesis genes, this study is conducted to shed some light on mechanisms and the interference of steroid hormones and antibiotics.Materials, Methods & Results: In this study, male Wistar rats were injected intramuscularly with Testosterone, progesterone and estradiol; while tetracycline and streptomycin injections were intraperitoneal. Testosterone, progesterone and estradiol injections were carried out in a short-term (15 days) and long-term (45 days) periods. Steroid hormones were dissolved in sesame in a way that by each injection, 0.2 mL sesame oil (containing specific amount of hormone) was injected to the rat. Control group was kept in the same condition except that their sesame oil injection contained no hormone. Tetracycline and Streptomycin injection was carried out for 5 days at 7 am and pm, at 50 mg/kg dosage intraperitoneally. In short- and long-term periods, rats were divided into four groups of 6-member. The concentrations were the same in the periods and 0.2 mL sesame oil was injected intramuscularly. 1 mg testosterone, 12 mg progesterone and 0.2 mg estradiol were intramuscularly injected to rats in group 2, 3 and 4, respectively [10]. Rats were divided into 9 six-member groups as follows: Group 1: intraperitoneal injection of 0.2 mL physiological serum; group 2: injection of 1 mg testosterone; group 3: injection of 1 mg testosterone + 50 mg/kg streptomycin; group 4: injection of 1 mg testosterone + 50 mg/kg tetracycline; group 5: injection of 0.2 mg estradiol; group 6: injection of 0.2 mg estradiol + 50 mg/kg streptomycin; group 7: injection of 0.2 mg estradiol + 50 mg/kg tetracycline; group 8: injection 50 mg/kg streptomycin; and group 9: injection of 50 mg/kg tetracycline. Serum concentration of AST enzyme was measured at the end of each period and the data were compared by SPSS software. all three steroid hormones had no significant impact on AST activity in short term. However, a significant effect was observed in long term in mean AST activities of the 4 groups. The group injected by testosterone exhibited 9% increases in comparison with the control group. Antibiotic-administrated groups showed lower activities as compared with hormone-injected groups. Steroid hormones and testosterone can enhance AST activity, in short-term and long-term, respectively by induction of protein enzyme. The second test confirmed this theory as the antibiotics decreased the AST activity enhancement by testosterone.Discussion: Based on the present study, steroid hormones can enhance the aspartate aminotransferase activity; and antibiotics can decrease the level of this liver enzyme by inhibition of polypeptide synthesis on related genes. This reaction could be due to interference of hormones and antibiotics effect which hinders the hormone effect along with the drug to activate the protein synthesis process.

EFFECTS OF OVARIAN STEROID HORMONES ON HISTOCHEMICALLY DEMONSTRABLE LIPIDS IN THE RAT UTERINE EPITHELIUM

1973 ◽  
Vol 56 (1) ◽  
pp. 59-67 ◽  
Author(s):  
D. P. BOSHIER ◽  
HILARY HOLLOWAY
Keyword(s):  
Steroid Hormones ◽  
Medroxyprogesterone Acetate ◽  
Neutral Lipids ◽  
Uterine Epithelium ◽  
Oestrous Cycle ◽  
Ovarian Steroid ◽  
Plasma Progesterone ◽  
Unsaturated Lipids ◽  
High Concentrations

SUMMARY Histochemical studies showed that during the rat oestrous cycle, uterine epithelial lipids were at their highest levels during dioestrus and pro-oestrus. In ovariectomized mature animals given oestradiol-17β, either by itself or in combination with medroxyprogesterone acetate, neutral lipids and unsaturated lipids were below control levels, while phospholipids were increased. Medroxyprogesterone acetate, however, increased levels of neutral lipids and unsaturated lipids, and decreased phospholipids. It is suggested that during the oestrous cycle of the rat, high concentrations of uterine epithelial lipid during late dioestrus and pro-oestrus reflect the increased plasma progesterone of early dioestrus acting in a low plasma oestrogen environment.

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