Diffusion Rate of Adrenal Steroids across the whole Human Amniotic Membrane in vitro

2015 ◽  
pp. 55-62
Author(s):  
P.A. Osinski
Keyword(s):  
Amniotic Membrane ◽  
Diffusion Rate ◽  
Human Amniotic Membrane ◽  
Adrenal Steroids

scholarly journals A Novel Phenotype of Junctional Epidermolysis Bullosa with Transient Skin Fragility and Predominant Ocular Involvement Responsive to Human Amniotic Membrane Eyedrops

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 716
Author(s):  
Daniele Castiglia ◽  
Paola Fortugno ◽  
Angelo Giuseppe Condorelli ◽  
Sabina Barresi ◽  
Naomi De Luca ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Epidermolysis Bullosa ◽  
Ocular Involvement ◽  
Human Amniotic Membrane ◽  
Vitro Assay ◽  
Junctional Epidermolysis Bullosa ◽  
Mucosal Involvement ◽  
Skin Fragility ◽  
Laminin 332

Junctional epidermolysis bullosa (JEB) is a clinically and genetically heterogeneous skin fragility disorder frequently caused by mutations in genes encoding the epithelial laminin isoform, laminin-332. JEB patients also present mucosal involvement, including painful corneal lesions. Recurrent corneal abrasions may lead to corneal opacities and visual impairment. Current treatments are merely supportive. We report a novel JEB phenotype distinguished by the complete resolution of skin fragility in infancy and persistent ocular involvement with unremitting and painful corneal abrasions. Biallelic LAMB3 mutations c.3052-5C>G and c.3492_3493delCG were identified as the molecular basis for this phenotype, with one mutation being a hypomorphic splice variant that allows residual wild-type laminin-332 production. The reduced laminin-332 level was associated with impaired keratinocyte adhesion. Then, we also investigated the therapeutic power of a human amniotic membrane (AM) eyedrop preparation for corneal lesions. AM were isolated from placenta donors, according to a procedure preserving the AM biological characteristics as a tissue, and confirmed to contain laminin-332. We found that AM eyedrop preparation could restore keratinocyte adhesion in an in vitro assay. Of note, AM eyedrop administration to the patient resulted in long-lasting remission of her ocular manifestations. Our findings suggest that AM eyedrops could represent an effective, non-invasive, simple-to-handle treatment for corneal lesions in patients with JEB and possibly other EB forms.

In Vitro Evaluation of the Drug Reservoir Function of Human Amniotic Membrane Using Moxifloxacin as a Model Drug

Cornea ◽  
2017 ◽  
Vol 36 (5) ◽  
pp. 594-599 ◽  
Author(s):  
Madhavi Latha Yelchuri ◽  
Bhagyashree Madhavi ◽  
Nilam Gohil ◽  
Hitha Sara Sajeev ◽  
Namperumalsamy Venkatesh Prajna ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
In Vitro Evaluation ◽  
Reservoir Function ◽  
Drug Reservoir ◽  
Model Drug

scholarly journals An In vitro porcine study of repearing articular cartilage with human amniotic membrane epithelial and mesenchymal stem cells

2012 ◽  
Vol 20 ◽  
pp. S273
Author(s):  
E. Muiños-López ◽  
S.M. Díaz-Prado ◽  
T. Hermida-Gómez ◽  
E. Rendal-Vázquez ◽  
I. Fuentes-Boquete ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

scholarly journals The Osteogenic Capacity of HumanAmniotic Membrane Mesenchymal Stem Cell (hAMSC) and Potential for Application in Maxillofacial Bone Reconstruction in Vitro Study

2020 ◽  
Vol 4 (1) ◽  
pp. 17
Author(s):  
David Kamadjaja
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Amniotic Membrane ◽  
Isolation Procedure ◽  
Target Cells ◽  
Human Amniotic Membrane ◽  
Bone Reconstruction ◽  
Differentiation Potential

Amniotic membrane of human placenta is a source of abundant mesenchymal stem cell (hAMSC) which makes it a potential source of allogeneic multipotent cell for bone healing.  However, much has to be explored about its isolation procedure and the osteogenic differentiation potential. The aims of this study are to establish the procurement procedure of human amniotic membrane, the isolation   and culture   of hAMSC, the MSC phenotypic characterization, and the in vitro osteogenic differentiation of hAMSC.  Results of the study are as follows. The quality of human amniotic membrane would be best if procured from Caesarean operation under highly aseptic condition to avoid fungal and bacterial contamination on the culture.  Isolation procedure using modified   Soncini protocol yielded large amount of MSC with high proliferative capacity in culture medium.  Characterization of hAMSC showed that the majority of the target cells exhibited specific MSC markers (CD10S and CD90) with a small number of these cells expressing CD45the marker of hematopoeitic cells. The in vitro osteogenic differentiation of hAMSC  followed by Alizarin  Red staining showed that  osteoblastic differentiation  was  detected in a significantly   high  number  of cells.  This study concludes that hAMSCs isolated from human amniotic membrane have the capacity for in vitro osteogenesis which makes them be one of the potential allogeneic stem cells for application in maxillofacial bone reconstruction.

scholarly journals Biocompatibility of quantum dots (CdSe/ZnS ) in human amniotic membrane-derived mesenchymal stem cells in vitro

2015 ◽  
Vol 159 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Gongping Wang ◽  
Guangwei Zeng ◽  
Caie Wang ◽  
Huasheng Wang ◽  
Bo Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Mesenchymal Stem Cells ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

In vitro osteodifferentiation of intact human amniotic membrane is not beneficial in the context of bone repair

2019 ◽  
Vol 20 (3) ◽  
pp. 435-446
Author(s):  
Thomas Gualdi ◽  
Romain Laurent ◽  
Virginie Moutarlier ◽  
Mathilde Fenelon ◽  
Aurélie Nallet ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Bone Repair ◽  
Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

10.3791/60551 ◽  
2019 ◽  
Author(s):  
Daniela Avila-González ◽  
Guadalupe García-López ◽  
Néstor E. Díaz-Martínez ◽  
Héctor Flores-Herrera ◽  
Anayansi Molina-Hernández ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Culture ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

Design of Experiment, Preparation, and in vitro Biological Assessment of Human Amniotic Membrane Extract Loaded Nanoparticles

2020 ◽  
Vol 21 (3) ◽  
pp. 256-267 ◽  
Author(s):  
Avishan Shabani ◽  
Fatemeh Atyabi ◽  
Mohammad R. Khoshayand ◽  
Reza Mahbod ◽  
Reza A. Cohan ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Ocular Surface ◽  
Dextran Sulfate ◽  
Biological Assessment ◽  
Human Amniotic Membrane ◽  
Infectious Risk ◽  
Angiogenic Effect ◽  
Membrane Extract ◽  
Average Size

Background: Human amniotic membrane grafting could be potentially useful in ocular surface complications due to tissue similarity and the presence of factors that reduce inflammation, vascularization, and scarring. However, considerations like donor-derived infectious risk and the requirement of an invasive surgery limit the clinical application of such treatments. Moreover, the quick depletion of bioactive factors after grafting reduces the efficacy of treatments. Therefore, in the current study, the possibility of nano delivery of the bioactive factors extracted from the human amniotic membrane to the ocular surface was investigated. Materials and methods: Nanoparticles were prepared using polyelectrolyte complexation from chitosan and dextran sulfate. The effect of polymer ratio, pH, and the amount of extract on particle size and encapsulation efficacy were studied using Box-Behnken response surface methodology. Results: The optimum condition was obtained as follows: 4.9:1 ratio of dextran sulfate to chitosan, 600 µL amniotic membrane extract, and pH of 6. The prepared nanoparticles had an average size of 213 nm with 77% encapsulation efficacy. In the release test, after 10 days, approximately 50% of entrapped bioactive proteins were released from the nanocarriers in a controlled manner. Biological activity assessment on endothelial cells revealed amniotic membrane extract loaded nanoparticles had a longer and significant increase in anti-angiogenic effect when compared to the control. Conclusion: Our data elucidate the ability of nanotechnology in ocular targeted nano delivery of bioactive compounds.

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