Multilamellar Liposome Preparation and Surface Activity of Synthetic Phospholipids

Author(s):  
M. Obladen ◽  
I. Klatt ◽  
M. Bartholom�
2020 ◽  
Vol 10 (3) ◽  
pp. 228-236 ◽  
Author(s):  
Lamia Taouzinet ◽  
Sofiane Fatmi ◽  
Allaeddine Khellouf ◽  
Mohamed Skiba ◽  
Mokrane Iguer-ouada

Background: Alpha-tocopherol is a potent antioxidant involved in sperm protection particularly during cryopreservation. However, its poor solubility limits the optimal protection in aqueous solutions. Objective: The aim of this study was to enhance the solubility of α-tocopherol by the use of liposomes. Methods: The experimental approach consisted to load vitamin E in liposomes prepared by ethanol injection method and the optimization carried out by an experimental design. The optimum solution was characterized by high performance liquid chromatography and scanning electron microscope. Finely, the impact on sperm motility protection was studied by the freezing technic of bovine sperm. Results: The optimum solution was obtained when using 10.9 mg/ml of phospholipids, 1.7 mg/ml of cholesterol and 2 mg/ml of vitamin E. The liposome size was 99.86 nm, providing 78.47% of loaded efficiency. The results showed also a significant positive impact on sperm motility after hours of preservation. Conclusion: In conclusion, the current results showed the interest of liposome preparation as an alternative to enhance vitamin E solubility and to protect spermatozoa during cryopreservation.


1993 ◽  
Vol 58 (3) ◽  
pp. 496-505
Author(s):  
Ondřej Wein

Partial blocking of the transport surface under the stagnant (nerst) layer is simulated by periodically alternating bands of perfectly insulating zones and active zones with a constant potential of driving force. The numeric solution of the corresponding two-dimensional elliptic problem is represented by a simple empirical correlation for the transfer coefficients. The result is interpreted in terms of a simple electrochemical problem about limiting diffusion currents at electrodes with non-uniform surface activity.


2021 ◽  
Vol 334 ◽  
pp. 115988
Author(s):  
Angelica Mero ◽  
Andrea Mezzetta ◽  
Janusz Nowicki ◽  
Justyna Łuczak ◽  
Lorenzo Guazzelli

2021 ◽  
Vol 489 ◽  
pp. 229406
Author(s):  
Nana Sun ◽  
Dayu Zhou ◽  
Wenwen Liu ◽  
Aikui Li ◽  
Yunpeng Su ◽  
...  

2021 ◽  
Vol 13 (15) ◽  
pp. 8122
Author(s):  
Shijie Tian ◽  
Weiqiang Tan ◽  
Xinyuan Wang ◽  
Tingting Li ◽  
Fanhao Song ◽  
...  

Surface activity of humic acid (HA) and its six sub-fractions isolated from forest soil were characterized by surface tension measurements, dynamic light scattering, and laser doppler electrophoresis. The surface tension of HA and its sub-fractions reduced from 72.4 mN·m−1 to 36.8 mN·m−1 in exponential model with the increasing concentration from 0 to 2000 mg·L−1. The critical micelle concentration (CMC) and Z-average particle size ranged from 216–1024 mg·L−1 and 108.2–186.9 nm for HA and its sub-fractions, respectively. The CMC have related with alkyl C, O-alkyl C, aromatic C, and carbonyl C (p < 0.05), respectively, and could be predicted with the multiple linear regression equation of CMC, CMC = 18896 − 6.9 × C-296 × alkyl C-331 × aromatic C-17019 × H/C + 4054 × HB/HI (p < 0.05). The maximum particle size was 5000 nm after filtered by a membrane with pore size of 450 nm, indicating HA and its sub-fractions could progressed self-assembly at pH 6.86. The aggregate sizes of number-base particle size distributions were mainly in six clusters including 2 ± 1 nm, 5 ± 2 nm, 10 ± 3 nm, 21 ± 8 nm, 40 ± 10 nm, and >50 nm analyzed by Gaussian model that maybe due to the inconsistency of the components and structures of the HA sub-fractions, requiring further study. It is significance to explore the surface activity of HA and its sub-fractions, which is helpful to clarify the environmental behavior of HA.


RSC Advances ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 1750-1761
Author(s):  
M. M. Abdelhamid ◽  
S. A. Rizk ◽  
M. A. Betiha ◽  
S. M. Desouky ◽  
A. M. Alsabagh

This study focuses on preparing a new family of organometallic surfactants based on five ion complexes, namely Co2+, Ni2+, Cu2+, Fe3+, and Mn2+.


Sign in / Sign up

Export Citation Format

Share Document