Basal Secretion of Peptides Derived from the ACTH/Endorphin Precursor by Rat Pituitary Cells in Culture1

2015 ◽  
pp. 12-20
Author(s):  
B. A. Eipper ◽  
R. E. Mains
Keyword(s):  
Pituitary Cells ◽  
Basal Secretion ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

INHIBIN FROM CULTURES OF RAT SEMINIFEROUS TUBULES

1978 ◽  
Vol 78 (2) ◽  
pp. 217-224 ◽  
Author(s):  
L. W. EDDIE ◽  
H. W. G. BAKER ◽  
A. DULMANIS ◽  
R. E. HIGGINSON ◽  
B. HUDSON
Keyword(s):  
Luteinizing Hormone ◽  
Active Material ◽  
Gel Filtration ◽  
Seminiferous Tubules ◽  
Pituitary Cells ◽  
Basal Secretion ◽  
Luteinizing Hormone Releasing Hormone ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Lh Rh

SUMMARY Medium from cultures of mature rat seminiferous tubules contained a substance which suppressed, in a dose-related manner, the luteinizing hormone releasing hormone (LH-RH)-stimulated secretion of FSH by cultured rat pituitary cells. The secretion of LH was suppressed to a lesser extent and the basal secretion of both LH and FSH was inconsistently affected. Gel filtration on Sephadex G-100 did not fractionate the activity. The active material did not inhibit the secretion of TSH or destroy LH-RH and the activity was not due to testosterone or oestradiol in the medium. Control media from liver cultures were inactive. It is concluded that inhibin is present in media from cultures of rat seminiferous tubules.

EFFECTS OF GONADAL STEROIDS AND CYCLOHEXIMIDE ON THE RELEASE OF GONADOTROPHINS BY RAT PITUITARY CELLS IN CULTURE

1978 ◽  
Vol 77 (3) ◽  
pp. 409-415 ◽  
Author(s):  
LUCIANO DEBELJUK ◽  
ASHOK KHAR ◽  
MARIAN JUTISZ
Keyword(s):  
Steroid Treatment ◽  
Gonadal Steroids ◽  
Control Group ◽  
Pituitary Cells ◽  
Basal Secretion ◽  
Rat Pituitary ◽  
Basal Release ◽  
Gonadotrophin Releasing Hormone ◽  
Rat Pituitary Cells ◽  
Dose Levels

The effects of oestradiol-17β, testosterone and progesterone alone and together with cycloheximide on the basal and gonadotrophin releasing hormone (GnRH)-induced release of gonadotrophins were studied in cultured dispersed rat pituitary cells. In the control group (no steroid treatment), GnRH significantly stimulated the release of LH and FSH; cycloheximide partially inhibited this response, although it had no effect on the basal secretion of gonadotrophins. A dose of 5 ng oestradiol/ml had no significant effect on the response to GnRH; at a dose of 100 ng/ml the GnRH-induced release of LH was significantly augmented whereas the release of FSH was inhibited. Cycloheximide blocked the augmenting effect of oestradiol. The basal release of LH was slightly but significantly inhibited in response to 10 ng testosterone/ml and increased in response to progesterone (200 ng/ml). Testosterone at both dose levels and progesterone significantly inhibited the GnRHinduced release of LH and FSH and in testosterone and progesterone-treated groups, the response to GnRH was inhibited by cycloheximide, but not beyond the levels observed in the control group. It is concluded that steroids can act directly on the pituitary cells, that oestradiol stimulates the GnRH-induced release of LH and that cycloheximide blocks this stimulatory effect. Testosterone and progesterone, on the other hand, partially inhibit the response to GnRH.

SPONTANEOUS RELEASE OF IMMUNOREACTIVE ACTH FROM ISOLATED RAT PITUITARY CELLS

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S162
Author(s):  
H. L. Fehm ◽  
K. H. Voigt ◽  
R. Lang ◽  
M. Schleyer ◽  
E. F. Pfeiffer
Keyword(s):  
Pituitary Cells ◽  
Spontaneous Release ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Isolated Rat

Modulation of prolactin secretion by contraceptive progestins in cultured rat pituitary cells in vitro

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S188-S189
Author(s):  
L. KIESEL ◽  
T. RABE ◽  
D. SCHOLZ ◽  
V. KIRSCHNER ◽  
B. RUNNEBAUM
Keyword(s):  
Prolactin Secretion ◽  
Pituitary Cells ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

Effects of chlorobenzenes on the histamine (HA) activated arginine-vasopressin (AVP) release -- from rat pituitary cells

2013 ◽  
Author(s):  
Zsuzsanna Valkusz ◽  
Zsolt Molnar ◽  
Peter Hausinger ◽  
Mariann Radacs ◽  
Marta Galfi ◽  
...  
Keyword(s):  
Arginine Vasopressin ◽  
Pituitary Cells ◽  
Rat Pituitary ◽  
Rat Pituitary Cells
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