Effects of testosterone on hormonal content and calcium-dependent basal secretion in female rat pituitary cells

2007 ◽  
Vol 103 (2) ◽  
pp. 149-157 ◽  
Author(s):  
Juergen M. Weiss ◽  
Stanko S. Stojilkovic ◽  
Klaus Diedrich ◽  
Olaf Ortmann
Keyword(s):  
Pituitary Cells ◽  
Female Rat ◽  
Basal Secretion ◽  
Calcium Dependent ◽  
Rat Pituitary ◽  
Hormonal Content ◽  
Rat Pituitary Cells

scholarly journals Effects of activin on hormone secretion by single female rat pituitary cells: analysis by cell immunoblot assay

1999 ◽  
Vol 161 (3) ◽  
pp. 375-382 ◽  
Author(s):  
S Miyamoto ◽  
M Irahara ◽  
K Ushigoe ◽  
A Kuwahara ◽  
H Sugino ◽  
...  
Keyword(s):  
Hormone Secretion ◽  
Activin A ◽  
Female Rats ◽  
Pituitary Cells ◽  
Female Rat ◽  
Single Female ◽  
Immunoblot Assay ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Lh Secretion

We investigated the effect of activin A on secretion of LH, FSH, and prolactin (PRL) by female cultured rat pituitary cells at the single-cell level by means of the cell immunoblot assay. Anterior pituitary cells from 8-week-old female rats were preincubated with or without activin A for 24 h, after which they were monodispersed and immediately used for cell immunoblot assay. The percentages of LH-, FSH- and PRL-immunoreactive cell blots detected were 5.5, 5.3 and 43.1%, respectively, of all pituitary cells applied to the transfer membrane. The percentage of LH-secreting cells and mean LH secretion per cell did not change after treatment with activin. In contrast, activin significantly increased the percentage of FSH-secreting cells and mean FSH secretion per cell to 136.0 and 114. 5% respectively. In addition, activin significantly decreased the percentage of PRL-secreting cells and mean PRL secretion per cell to 52.2 and 72.0% respectively. These results suggest that (1) activin A has effects on female rat pituitary cells that increase not only the amount of FSH secretion per cell but also the number of FSH-secreting cells, and (2) activin A decreases both the amount of PRL secretion per cell and the number of PRL-secreting cells.

INHIBIN FROM CULTURES OF RAT SEMINIFEROUS TUBULES

1978 ◽  
Vol 78 (2) ◽  
pp. 217-224 ◽  
Author(s):  
L. W. EDDIE ◽  
H. W. G. BAKER ◽  
A. DULMANIS ◽  
R. E. HIGGINSON ◽  
B. HUDSON
Keyword(s):  
Luteinizing Hormone ◽  
Active Material ◽  
Gel Filtration ◽  
Seminiferous Tubules ◽  
Pituitary Cells ◽  
Basal Secretion ◽  
Luteinizing Hormone Releasing Hormone ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Lh Rh

SUMMARY Medium from cultures of mature rat seminiferous tubules contained a substance which suppressed, in a dose-related manner, the luteinizing hormone releasing hormone (LH-RH)-stimulated secretion of FSH by cultured rat pituitary cells. The secretion of LH was suppressed to a lesser extent and the basal secretion of both LH and FSH was inconsistently affected. Gel filtration on Sephadex G-100 did not fractionate the activity. The active material did not inhibit the secretion of TSH or destroy LH-RH and the activity was not due to testosterone or oestradiol in the medium. Control media from liver cultures were inactive. It is concluded that inhibin is present in media from cultures of rat seminiferous tubules.

Oestrogen receptor α and β in female rat pituitary cells: An immunochemical study

2008 ◽  
Vol 155 (3) ◽  
pp. 857-868 ◽  
Author(s):  
Miriam González ◽  
Ricardo Reyes ◽  
Carmen Damas ◽  
Rafael Alonso ◽  
Aixa R. Bello
Keyword(s):  
Oestrogen Receptor ◽  
Pituitary Cells ◽  
Female Rat ◽  
Immunochemical Study ◽  
Rat Pituitary ◽  
Oestrogen Receptor Α ◽  
Rat Pituitary Cells

EFFECTS OF GONADAL STEROIDS AND CYCLOHEXIMIDE ON THE RELEASE OF GONADOTROPHINS BY RAT PITUITARY CELLS IN CULTURE

1978 ◽  
Vol 77 (3) ◽  
pp. 409-415 ◽  
Author(s):  
LUCIANO DEBELJUK ◽  
ASHOK KHAR ◽  
MARIAN JUTISZ
Keyword(s):  
Steroid Treatment ◽  
Gonadal Steroids ◽  
Control Group ◽  
Pituitary Cells ◽  
Basal Secretion ◽  
Rat Pituitary ◽  
Basal Release ◽  
Gonadotrophin Releasing Hormone ◽  
Rat Pituitary Cells ◽  
Dose Levels

The effects of oestradiol-17β, testosterone and progesterone alone and together with cycloheximide on the basal and gonadotrophin releasing hormone (GnRH)-induced release of gonadotrophins were studied in cultured dispersed rat pituitary cells. In the control group (no steroid treatment), GnRH significantly stimulated the release of LH and FSH; cycloheximide partially inhibited this response, although it had no effect on the basal secretion of gonadotrophins. A dose of 5 ng oestradiol/ml had no significant effect on the response to GnRH; at a dose of 100 ng/ml the GnRH-induced release of LH was significantly augmented whereas the release of FSH was inhibited. Cycloheximide blocked the augmenting effect of oestradiol. The basal release of LH was slightly but significantly inhibited in response to 10 ng testosterone/ml and increased in response to progesterone (200 ng/ml). Testosterone at both dose levels and progesterone significantly inhibited the GnRHinduced release of LH and FSH and in testosterone and progesterone-treated groups, the response to GnRH was inhibited by cycloheximide, but not beyond the levels observed in the control group. It is concluded that steroids can act directly on the pituitary cells, that oestradiol stimulates the GnRH-induced release of LH and that cycloheximide blocks this stimulatory effect. Testosterone and progesterone, on the other hand, partially inhibit the response to GnRH.

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