Changes in Dental Plaque Microbial Richness and Oral Behavioral Habits during Caries Development in Young Chinese Children

2015 ◽  
Vol 49 (2) ◽  
pp. 116-123 ◽  
Author(s):  
Wenjing Hao ◽  
He Xu ◽  
Xiaochi Chen ◽  
Qiong Zhou ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Free Group ◽  
High Frequency ◽  
Gel Electrophoresis ◽  
Dental Plaque ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Chinese Children ◽  
Supragingival Plaque ◽  
Gradient Gel Electrophoresis ◽  
Total Dna

Objective: To detect changes in the microbial richness of dental plaque and oral behaviors during caries development in young Chinese children. Methods: Supragingival plaque samples and a survey of oral behaviors of 130 children aged 3 at baseline were analyzed at 6 months and 12 months. Total DNA was isolated from all samples and PCR-denaturing gradient gel electrophoresis analysis was conducted. Results: In the follow-up, 44 children had caries or cavity fillings at 6 months, a further 28 children had caries or cavity fillings at 12 months. The other 58 children remained caries-free at 12 months. According to the changes in caries status at the 12-month follow-up, all participants were divided into three groups: caries-free, caries at 6 months and caries at 12 months. The changes in oral behaviors during the 12-month follow-up were not significantly different in the three groups. The frequency of eating sweets and eating sweets before sleeping was significantly different among the three groups at baseline. At baseline, the average detectable bands of caries in the 12-month caries group were similar to those of the caries-free group; both of them were higher than that of the 6-month caries group. At 6 months, the average detectable bands of the 12-month caries group were significantly lower than that of the caries-free group although the children of the 12-month caries group were caries-free at that time. Conclusions: For young Chinese children, the high frequency of eating sweets and eating sweets before sleeping are risk factors of caries onset, and the decrease in microbial richness could occur 6 months before the onset of caries.

scholarly journals Bacterial population profiling from diesel obtained from fuel stations

2016 ◽  
Vol 35 (1) ◽  
Author(s):  
Eduard Venter ◽  
Phumzile Sibisi ◽  
Natasha Van de Haar ◽  
Adriaana Jacobs
Keyword(s):  
Gel Electrophoresis ◽  
Bacillus Pumilus ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Culture Media ◽  
Bacterial Species ◽  
Diesel Fuels ◽  
Polycyclic Aromatic ◽  
Gradient Gel Electrophoresis ◽  
Identification Of Bacteria ◽  
Total Dna

Microbial growth develops in stored diesel fuel. This can influence later use in emergency backup generators and vehicles through the clogging of fuel filters by the development of biomass. A mutualistic microbial community can develop in stored fuel products that degrade polycyclic aromatic hydrocarbons to the benefit of one another. This project aimed to study the bacterial community profile of diesel obtained from commercial fuel stations using culture dependent and independent methods. Bacteria and total DNA were isolated from the diesel and the community was studied using denaturing gradient gel electrophoresis of the 16S rDNA region. This resulted in the isolation of six bacterial species from two different genera (related to Bacillus and Lysinibacillus) that could grow in pure culture with diesel as carbon source. The denaturing gradient gel electrophoresis analysis indicated the occurrence of an additional eight genera in the sampled diesel. Both methods identified bacteria related to Bacillus pumilus occurring in the samples. Bacillus pumilus was the predominant species (50%) isolated from diesel samples using culture media. The identification of bacteria occurring in commercial diesel samples can be useful in determining the degree of degradation occurring during the storage of the product and also possible bioremediation agents in diesel fuels spills.

Denaturing gradient gel electrophoresis identifies genomic DNA polymorphism with high frequency in maize

1990 ◽  
Vol 80 (1) ◽  
pp. 1-10 ◽  
Author(s):  
G. E. Riedel ◽  
S. L. Swanberg ◽  
K. D. Kuranda ◽  
K. Marquette ◽  
P. LaPan ◽  
...  
Keyword(s):  
High Frequency ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Dna Polymorphism ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis

Molecular assessment of salt-tolerant, perchlorate- and nitrate-reducing microbial cultures

2009 ◽  
Vol 60 (7) ◽  
pp. 1745-1756 ◽  
Author(s):  
G. Zuo ◽  
D. J. Roberts ◽  
S. G. Lehman ◽  
G. W. Jackson ◽  
G. E. Fox ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Defined Medium ◽  
Salt Tolerant ◽  
Rdna Genes ◽  
Enrichment Cultures ◽  
Gradient Gel Electrophoresis ◽  
Total Dna ◽  
Molecular Assessment

The microbial ecology of enrichment cultures adapted to the removal of perchlorate and nitrate from high salt solutions and ion-exchange brines was examined over a period of four years using denaturing gradient gel electrophoresis and total DNA extraction with cloning and in each case partial sequencing of the 16S rDNA genes. The cultures studied were a result of enrichment from marine sediment inoculum initiated in 2001. The resulting enrichment cultures were fed perchlorate, or perchlorate and nitrate, in a 3% (w/v) NaCl defined medium or ion-exchange brines (5.6% NaCl) containing perchlorate and nitrate with acetate as the electron donor. All of the sequences' closest matches in the NCBI GenBank database were to marine or salt-tolerant organisms. Strains belonging to the genera Halomonas or Marinobacter were found to dominate in cultures that were fed nitrate in addition to perchlorate, but were effectively absent from cultures fed perchlorate alone. The cultures fed perchlorate as the sole electron acceptor were relatively diverse with the dominant sequences belonging to the genera Dechloromarinus and Denitromonas. A study examining the effects of growing the cultures on different electron acceptors to the cultures revealed that Denitromonas may be more dominant than Dechloromarinus as the salt-tolerant, perchlorate-reducing organism.

Allelopathic and Medicinal plant. 26. Millettia speciosa

2020 ◽  
Vol 51 (2) ◽  
pp. 125-146
Author(s):  
Nasiruddin Nasiruddin ◽  
Yu Zhangxin ◽  
Ting Zhao Chen Guangying ◽  
Minghui Ji
Keyword(s):  
Community Structure ◽  
Medicinal Plant ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wiener Index ◽  
Pseudomonas Spp ◽  
Evenness Index ◽  
Gradient Gel Electrophoresis ◽  
Rhizosphere Pseudomonas

We grew cucumber in pots in greenhouse for 9-successive cropping cycles and analyzed the rhizosphere Pseudomonas spp. community structure and abundance by PCR-denaturing gradient gel electrophoresis and quantitative PCR. Results showed that continuous monocropping changed the cucumber rhizosphere Pseudomonas spp. community. The number of DGGE bands, Shannon-Wiener index and Evenness index decreased during the 3rd cropping and thereafter, increased up to the 7th cropping, however, however, afterwards they decreased again. The abundance of Pseudomonas spp. increased up to the 5th successive cropping and then decreased gradually. These findings indicated that the structure and abundance of Pseudomonas spp. community changed with long-term cucumber monocropping, which might be linked to soil sickness caused by its continuous monocropping.

scholarly journals Impact of Intensive Land-Based Fish Culture in Qingdao, China, on the Bacterial Communities in Surrounding Marine Waters and Sediments

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Qiufen Li ◽  
Yan Zhang ◽  
David Juck ◽  
Nathalie Fortin ◽  
Charles W. Greer
Keyword(s):  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Fish Culture ◽  
Fish Ponds ◽  
Marine Area ◽  
Gradient Gel Electrophoresis ◽  
Reducing Bacteria ◽  
The Impact ◽  
Nitrate Reducing Bacteria

The impact of intensive land-based fish culture in Qingdao, China, on the bacterial communities in surrounding marine environment was analyzed. Culture-based studies showed that the highest counts of heterotrophic, ammonium-oxidizing, nitrifying, and nitrate-reducing bacteria were found in fish ponds and the effluent channel, with lower counts in the adjacent marine area and the lowest counts in the samples taken from 500 m off the effluent channel. Denaturing gradient gel electrophoresis (DGGE) analysis was used to assess total bacterial diversity. Fewer bands were observed from the samples taken from near the effluent channel compared with more distant sediment samples, suggesting that excess nutrients from the aquaculture facility may be reducing the diversity of bacterial communities in nearby sediments. Phylogenetic analysis of the sequenced DGGE bands indicated that the bacteria community of fish-culture-associated environments was mainly composed of Flavobacteriaceae, gamma- and deltaproteobacteria, including generaGelidibacter, Psychroserpen, Lacinutrix,andCroceimarina.

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