Denaturing gradient gel electrophoresis identifies genomic DNA polymorphism with high frequency in maize

1990 ◽  
Vol 80 (1) ◽  
pp. 1-10 ◽  
Author(s):  
G. E. Riedel ◽  
S. L. Swanberg ◽  
K. D. Kuranda ◽  
K. Marquette ◽  
P. LaPan ◽  
...  
Keyword(s):  
High Frequency ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Dna Polymorphism ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis

scholarly journals Rhmod phenotype: a parentage problem solved by denaturing gradient gel electrophoresis of genomic DNA

2020 ◽  
Vol 12 (4) ◽  
pp. 154-158
Author(s):  
Fiona J. Steers ◽  
Maura Wallace ◽  
Marialuisa Mora ◽  
Ben Carritt ◽  
Patricia Tippett ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gradient Gel Electrophoresis

Changes in Dental Plaque Microbial Richness and Oral Behavioral Habits during Caries Development in Young Chinese Children

2015 ◽  
Vol 49 (2) ◽  
pp. 116-123 ◽  
Author(s):  
Wenjing Hao ◽  
He Xu ◽  
Xiaochi Chen ◽  
Qiong Zhou ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Free Group ◽  
High Frequency ◽  
Gel Electrophoresis ◽  
Dental Plaque ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Chinese Children ◽  
Supragingival Plaque ◽  
Gradient Gel Electrophoresis ◽  
Total Dna

Objective: To detect changes in the microbial richness of dental plaque and oral behaviors during caries development in young Chinese children. Methods: Supragingival plaque samples and a survey of oral behaviors of 130 children aged 3 at baseline were analyzed at 6 months and 12 months. Total DNA was isolated from all samples and PCR-denaturing gradient gel electrophoresis analysis was conducted. Results: In the follow-up, 44 children had caries or cavity fillings at 6 months, a further 28 children had caries or cavity fillings at 12 months. The other 58 children remained caries-free at 12 months. According to the changes in caries status at the 12-month follow-up, all participants were divided into three groups: caries-free, caries at 6 months and caries at 12 months. The changes in oral behaviors during the 12-month follow-up were not significantly different in the three groups. The frequency of eating sweets and eating sweets before sleeping was significantly different among the three groups at baseline. At baseline, the average detectable bands of caries in the 12-month caries group were similar to those of the caries-free group; both of them were higher than that of the 6-month caries group. At 6 months, the average detectable bands of the 12-month caries group were significantly lower than that of the caries-free group although the children of the 12-month caries group were caries-free at that time. Conclusions: For young Chinese children, the high frequency of eating sweets and eating sweets before sleeping are risk factors of caries onset, and the decrease in microbial richness could occur 6 months before the onset of caries.

Allelopathic and Medicinal plant. 26. Millettia speciosa

2020 ◽  
Vol 51 (2) ◽  
pp. 125-146
Author(s):  
Nasiruddin Nasiruddin ◽  
Yu Zhangxin ◽  
Ting Zhao Chen Guangying ◽  
Minghui Ji
Keyword(s):  
Community Structure ◽  
Medicinal Plant ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wiener Index ◽  
Pseudomonas Spp ◽  
Evenness Index ◽  
Gradient Gel Electrophoresis ◽  
Rhizosphere Pseudomonas

We grew cucumber in pots in greenhouse for 9-successive cropping cycles and analyzed the rhizosphere Pseudomonas spp. community structure and abundance by PCR-denaturing gradient gel electrophoresis and quantitative PCR. Results showed that continuous monocropping changed the cucumber rhizosphere Pseudomonas spp. community. The number of DGGE bands, Shannon-Wiener index and Evenness index decreased during the 3rd cropping and thereafter, increased up to the 7th cropping, however, however, afterwards they decreased again. The abundance of Pseudomonas spp. increased up to the 5th successive cropping and then decreased gradually. These findings indicated that the structure and abundance of Pseudomonas spp. community changed with long-term cucumber monocropping, which might be linked to soil sickness caused by its continuous monocropping.

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