Maternal Plasma and Amniotic Fluid Cytokines in Monochorionic, Diamniotic Twin Pregnancies Complicated by Twin-to-Twin Transfusion Syndrome

2014 ◽  
Vol 35 (4) ◽  
pp. 280-288 ◽  
Author(s):  
Caroline E. Fox ◽  
Gendie E. Lash ◽  
Samantha J. Pretlove ◽  
Ben C. Chan ◽  
Roger Holder ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Maternal Plasma ◽  
Twin Pregnancies

scholarly journals Evaluation of a Microhaplotype-Based Noninvasive Prenatal Test in Twin Gestations: Determination of Paternity, Zygosity, and Fetal Fraction

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 26
Author(s):  
Zhaochen Bai ◽  
Hu Zhao ◽  
Shaobin Lin ◽  
Linhuan Huang ◽  
Zhiming He ◽  
...  
Keyword(s):  
Prenatal Testing ◽  
Maternal Plasma ◽  
Clinical Settings ◽  
Str Analysis ◽  
Forensic Research ◽  
Combined Use ◽  
Prenatal Test ◽  
Mz Twins ◽  
Twin Pregnancies ◽  
Fetal Fraction

As a novel type of genetic marker, the microhaplotype has shown promising potential in forensic research. In the present study, we analyzed maternal plasma cell-free DNA (cfDNA) samples from twin pregnancies to validate microhaplotype-based noninvasive prenatal testing (NIPT) for paternity, zygosity, and fetal fraction (FF). Paternity was determined with the combined use of the relMix package, zygosity was evaluated by examining the presence of informative loci with two fetal genome complements, and FF was assessed through fetal allele ratios. Paternity was determined in 19 twin cases, among which 13 cases were considered dizygotic (DZ) twins based on the presence of 3~10 informative loci and the remaining 6 cases were considered monozygotic (MZ) twins because no informative locus was observed. With the fetal genomic genotypes as a reference, the accuracy of paternity and zygosity determination were confirmed by standard short tandem repeat (STR) analysis. Moreover, the lower FF, higher FF, and combined FF in each DZ plasma sample were closely related to the estimated value. This present preliminary study proposes that microhaplotype-based NIPT is applicable for paternity, zygosity, and FF determination in twin pregnancies, which are expected to be advantageous for both forensic and clinical settings.

scholarly journals Testosterone measured from amniotic fluid and maternal plasma shows no significant association with directional asymmetry in newborn digit ratio (2D:4D)

2018 ◽  
Vol 10 (3) ◽  
pp. 362-367 ◽  
Author(s):  
G. Richards ◽  
M. Gomes ◽  
T. Ventura
Keyword(s):  
Amniotic Fluid ◽  
Secondary Analysis ◽  
Relative Length ◽  
Maternal Plasma ◽  
Mean Value ◽  
Digit Ratio ◽  
Directional Asymmetry ◽  
Second Trimester ◽  
Maternal Circulation ◽  
Left Hand

AbstractFoetal sex hormones can have powerful and far-reaching effects on later phenotype. However, obtaining accurate measurements is difficult for ethical reasons, and researchers often employ proxy variables to examine their effects. The relative length of the second and fourth fingers (digit ratio or 2D:4D) is frequently used for this purpose, as it is hypothesized to index variance in prenatal androgen and oestrogen exposure. Most studies employing this method examine digit ratio for the right hand (R2D:4D) and/or left hand (L2D:4D), though the mean value (M2D:4D) (i.e., the average of R2D:4D and L2D:4D) and directional asymmetry (D[R–L]) (i.e., R2D:4D minus L2D:4D) are also commonly used. As no published studies have examined M2D:4D or D[R-L] in relation to testosterone measured from amniotic fluid, we conducted a secondary analysis of data published by Ventura et al. The sample comprises 106 mothers from Portugal who underwent amniocentesis during the second trimester and their neonates. Newborn M2D:4D was negatively correlated with amniotic testosterone in females (P<0.05) but not in males; no significant association was observed between amniotic testosterone and D[R–L] in either sex. In addition, we examined testosterone measured from maternal circulation during the second trimester, and found that it was not a significant predictor of M2D:4D or D[R–L] in male or female infants. Further research should aim to measure the ratio of testosterone to oestradiol present in amniotic fluid and maternal plasma, to examine whether either is a predictor of digit ratio variables at different stages of postnatal development.

Effect of Amniotic Fluid Volume on Neonatal Outcome in Diamniotic Twin Pregnancies

1998 ◽  
Vol 91 (10) ◽  
pp. 942-945 ◽  
Author(s):  
EVERETT F. MAGANN ◽  
NEIL S. WIITWORTH ◽  
PHILIP G. RHODES ◽  
J. DAVID BASS ◽  
SUNEET P. CHAUHAN ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Neonatal Outcome ◽  
Fluid Volume ◽  
Amniotic Fluid Volume ◽  
Twin Pregnancies
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