scholarly journals A Comparative Study on Culture Conditions and Routine Expansion of Amniotic Fluid-Derived Mesenchymal Progenitor Cells

2013 ◽  
Vol 34 (4) ◽  
pp. 225-235 ◽  
Author(s):  
L. Gucciardo ◽  
N. Ochsenbein-Kölble ◽  
Y. Ozog ◽  
G. Verbist ◽  
V. Van Duppen ◽  
...  
Keyword(s):  
Comparative Study ◽  
Amniotic Fluid ◽  
Progenitor Cells ◽  
Culture Conditions ◽  
Mesenchymal Progenitor Cells

scholarly journals Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment

2018 ◽  
Vol 19 (9) ◽  
pp. 2549 ◽  
Author(s):  
Luisa Brandt ◽  
Susanna Schubert ◽  
Patrick Scheibe ◽  
Walter Brehm ◽  
Jan Franzen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Culture Conditions ◽  
Mesenchymal Progenitor Cells ◽  
Blood Leukocytes ◽  
Inflammatory Conditions ◽  
Tenogenic Differentiation ◽  
Adipose Derived Stromal Cells ◽  
Factor Α ◽  
The Impact

Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represented by supplementation with interleukin-1β and/or tumor necrosis factor-α or by co-culture with allogeneic peripheral blood leukocytes, on ASC functional properties was investigated. High cytokine concentrations increased ASC proliferation and osteogenic differentiation, but decreased chondrogenic differentiation and ASC viability in scaffold culture, as well as tendon scaffold repopulation, and strongly influenced musculoskeletal gene expression. Effects regarding the latter differed between the monolayer and scaffold cultures. Leukocytes rather decreased ASC proliferation, but had similar effects on viability and musculoskeletal gene expression. This included decreased expression of the tenogenic transcription factor scleraxis by an inflammatory environment throughout culture conditions. The data demonstrate that ASC tenogenic properties are compromised in an inflammatory environment, with relevance to their possible mechanisms of action in acute tendon disease.

scholarly journals In vitro and in vivo properties of distinct populations of amniotic fluid mesenchymal progenitor cells

2011 ◽  
Vol 15 (9) ◽  
pp. 1896-1913 ◽  
Author(s):  
Maria G. Roubelakis ◽  
Vasiliki Bitsika ◽  
Dimitra Zagoura ◽  
Ourania Trohatou ◽  
Kalliopi I. Pappa ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal progenitor cells with myogenic and neuronal properties

2007 ◽  
Vol 313 (5) ◽  
pp. 1008-1023 ◽  
Author(s):  
Mitsutaka Shiota ◽  
Toshio Heike ◽  
Munetada Haruyama ◽  
Shiro Baba ◽  
Atsunori Tsuchiya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document