scholarly journals Neural Ganglioside GD2+Cells Define a Subpopulation of Mesenchymal Stem Cells in Adult Murine Bone Marrow

2013 ◽  
Vol 32 (4) ◽  
pp. 889-898 ◽  
Author(s):  
Jie Xu ◽  
WenJun Fan ◽  
Xi Xiang Tu ◽  
Teng Zhang ◽  
Zhi Jie Hou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Ganglioside Gd2 ◽  
Murine Bone Marrow

Hyaluronic acid and chondrogenesis of murine bone marrow mesenchymal stem cells in chitosan sponges

2011 ◽  
Vol 72 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Zeev Schwartz ◽  
Dominique J. Griffon ◽  
L. Page Fredericks ◽  
Hae-Beom Lee ◽  
Hsin-Yi Weng
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Murine Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

scholarly journals An efficient method for isolation of murine bone marrow mesenchymal stem cells

2007 ◽  
Vol 51 (8) ◽  
pp. 723-729 ◽  
Author(s):  
Samad Nadri ◽  
Masoud Soleimani ◽  
Reza H. Hosseni ◽  
Mohammad Massumi ◽  
Amir Atashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Efficient Method ◽  
Murine Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

Purinergic Signaling Modulates Human Bone Marrow-Derived Mesenchymal Stem Cells Function.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1441-1441
Author(s):  
Valentina Salvestrini ◽  
Davide Ferrari ◽  
Sara Gulinelli ◽  
Luisa Caione ◽  
Wanda Piacibello ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Purinergic Signaling ◽  
Human Mesenchymal Stem Cells ◽  
Membrane Receptors ◽  
Extracellular Nucleotides ◽  
P2 Receptor ◽  
Murine Bone Marrow

Abstract Abstract 1441 Poster Board I-464 Nucleotides triphosphates are extracellular messengers binding to specific plasma membrane receptors (P2Rs) modulating a wide variety of biological responses in several cell types. In this study, we show that bone marrow-derived human mesenchymal stem cells (hMSCs) express several functional P2R subtypes at the molecular and protein level. hMSCs are very resistant to the cytotoxic effects of high concentrations of extracellular ATP, as demonstrated by the lack of morphological and mitochondrial changes or release of intracellular markers of cell death. Gene expression profiling revealed that hMSCs stimulated with ATP underwent a down-regulation of genes involved in cell proliferation, whereas those involved in cell migration were strongly up-regulated. Functional studies confirmed the inhibitory activity of ATP on proliferation of hMSCs and clonogenic stromal progenitors. Furthermore, ATP potentiated the chemotactic response of hMSCs to the chemokine CXCL12, and increased their spontaneous migration. In vivo, xenotransplant experiments demonstrated that the homing capacity of hMSCs to murine bone marrow was significantly increased by pre-treatment with ATP. Moreover ATP increased the production of the pro-inflammatory cytokines IL-2, IFN-gamma, and IL-12p70, while decreasing the anti-inflammatory cytokine IL-10. Thus, our data show that purinergic signaling modulates hMSCs functions and highlight a role for extracellular nucleotides in hMSCs biology. Disclosures Di Virgilio: F. Di Virgilio serves as a consultant for Cordex Pharma Inc. (USA) involved in the development of “P2 receptor-based drugs”. : Consultancy; F. Di Virgilio serves as a consultant for Affectis Pharmaceuticals AG (Germany), involved in the development of “P2 receptor-based drugs”. : Consultancy.

scholarly journals An Improved Harvest andin VitroExpansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Song Xu ◽  
Ann De Becker ◽  
Ben Van Camp ◽  
Karin Vanderkerken ◽  
Ivan Van Riet
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Surface Markers ◽  
Differentiation Potential ◽  
Murine Bone Marrow ◽  
Expansion Time ◽  
Expansion Protocol ◽  
In Vitro Expansion

Compared to bone marrow (BM) derived mesenchymal stem cells (MSCs) from human origin or from other species, the in vitro expansion and purification of murine MSCs (mMSCs) is much more difficult because of the low MSC yield and the unwanted growth of non-MSCs in the in vitro expansion cultures. We describe a modified protocol to isolate and expand murine BM derived MSCs based on the combination of mechanical crushing and collagenase digestion at the moment of harvest, followed by an immunodepletion step using microbeads coated with CD11b, CD45 and CD34 antibodies. The number of isolated mMSCs as estimated by colony forming unit-fibroblast (CFU-F) assay showed that this modified isolation method could yield 70.0% more primary colonies. After immunodepletion, a homogenous mMSC population could already be obtained after two passages. Immunodepleted mMSCs (ID-mMSCs) are uniformly positive for stem cell antigen-1 (Sca-1), CD90, CD105 and CD73 cell surface markers, but negative for the hematopoietic surface markers CD14, CD34 and CD45. Moreover the immunodepleted cell population exhibits more differentiation potential into adipogenic, osteogenic and chondrogenic lineages. Our data illustrate the development of an efficient and reliable expansion protocol increasing the yield and purity of mMSCs and reducing the overall expansion time.

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