Ligation of TLR2 and TLR4 on murine bone marrow-derived mesenchymal stem cells triggers differential effects on their immunosuppressive activity

2011 ◽  
Vol 271 (1) ◽  
pp. 147-156 ◽  
Author(s):  
Junxia Lei ◽  
Zhen Wang ◽  
Dayang Hui ◽  
Weihua Yu ◽  
Dunhua Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Immunosuppressive Activity ◽  
Differential Effects ◽  
Murine Bone Marrow

Hyaluronic acid and chondrogenesis of murine bone marrow mesenchymal stem cells in chitosan sponges

2011 ◽  
Vol 72 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Zeev Schwartz ◽  
Dominique J. Griffon ◽  
L. Page Fredericks ◽  
Hae-Beom Lee ◽  
Hsin-Yi Weng
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Murine Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Neural Ganglioside GD2+Cells Define a Subpopulation of Mesenchymal Stem Cells in Adult Murine Bone Marrow

2013 ◽  
Vol 32 (4) ◽  
pp. 889-898 ◽  
Author(s):  
Jie Xu ◽  
WenJun Fan ◽  
Xi Xiang Tu ◽  
Teng Zhang ◽  
Zhi Jie Hou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Ganglioside Gd2 ◽  
Murine Bone Marrow

scholarly journals An efficient method for isolation of murine bone marrow mesenchymal stem cells

2007 ◽  
Vol 51 (8) ◽  
pp. 723-729 ◽  
Author(s):  
Samad Nadri ◽  
Masoud Soleimani ◽  
Reza H. Hosseni ◽  
Mohammad Massumi ◽  
Amir Atashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Efficient Method ◽  
Murine Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

Purinergic Signaling Modulates Human Bone Marrow-Derived Mesenchymal Stem Cells Function.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1441-1441
Author(s):  
Valentina Salvestrini ◽  
Davide Ferrari ◽  
Sara Gulinelli ◽  
Luisa Caione ◽  
Wanda Piacibello ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Purinergic Signaling ◽  
Human Mesenchymal Stem Cells ◽  
Membrane Receptors ◽  
Extracellular Nucleotides ◽  
P2 Receptor ◽  
Murine Bone Marrow

Abstract Abstract 1441 Poster Board I-464 Nucleotides triphosphates are extracellular messengers binding to specific plasma membrane receptors (P2Rs) modulating a wide variety of biological responses in several cell types. In this study, we show that bone marrow-derived human mesenchymal stem cells (hMSCs) express several functional P2R subtypes at the molecular and protein level. hMSCs are very resistant to the cytotoxic effects of high concentrations of extracellular ATP, as demonstrated by the lack of morphological and mitochondrial changes or release of intracellular markers of cell death. Gene expression profiling revealed that hMSCs stimulated with ATP underwent a down-regulation of genes involved in cell proliferation, whereas those involved in cell migration were strongly up-regulated. Functional studies confirmed the inhibitory activity of ATP on proliferation of hMSCs and clonogenic stromal progenitors. Furthermore, ATP potentiated the chemotactic response of hMSCs to the chemokine CXCL12, and increased their spontaneous migration. In vivo, xenotransplant experiments demonstrated that the homing capacity of hMSCs to murine bone marrow was significantly increased by pre-treatment with ATP. Moreover ATP increased the production of the pro-inflammatory cytokines IL-2, IFN-gamma, and IL-12p70, while decreasing the anti-inflammatory cytokine IL-10. Thus, our data show that purinergic signaling modulates hMSCs functions and highlight a role for extracellular nucleotides in hMSCs biology. Disclosures Di Virgilio: F. Di Virgilio serves as a consultant for Cordex Pharma Inc. (USA) involved in the development of “P2 receptor-based drugs”. : Consultancy; F. Di Virgilio serves as a consultant for Affectis Pharmaceuticals AG (Germany), involved in the development of “P2 receptor-based drugs”. : Consultancy.

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