Growth Factors and Tendon Healing

2011 ◽  
pp. 53-64 ◽  
Author(s):  
Francesco Oliva ◽  
Stefano Gatti ◽  
Giuseppe Porcellini ◽  
Nicholas R. Forsyth ◽  
Nicola Maffulli
Keyword(s):  
Growth Factors ◽  
Tendon Healing

Response of the Injured Tendon to Growth Factors in the Presence or Absence of the Paratenon

2018 ◽  
Vol 47 (2) ◽  
pp. 462-467 ◽  
Author(s):  
Sebastian A. Müller ◽  
Nicholas P. Quirk ◽  
Julia A. Müller-Lebschi ◽  
Patricia E. Heisterbach ◽  
Lutz Dürselen ◽  
...  
Keyword(s):  
Growth Factors ◽  
Achilles Tendon ◽  
Platelet Rich Plasma ◽  
Tendon Repair ◽  
Tendon Healing ◽  
Cross Sectional ◽  
Autologous Conditioned Serum ◽  
Injured Tendon ◽  
The Right ◽  
Exogenous Growth Factors

Background: The paratenon is important for Achilles tendon healing. There is much interest in the use of exogenous growth factors (GFs) as potential agents for accelerating the healing of damaged Achilles tendons. Purpose/Hypothesis: The present study used a rat model to study the responses of the injured Achilles tendon to GFs in the presence or absence of the paratenon. The hypothesis was that responses of the injured tendon to GFs would be lower in the absence of a paratenon. Study Design: Controlled laboratory study. Methods: A 4-mm defect was created in the right Achilles tendon of 60 skeletally mature rats, which were treated with a validated combination of GFs (bFGF, BMP-12, and TGF-β1). Animals were randomly assigned to the intact paratenon (IP) group or resected paratenon (RP) group. Healing was studied anatomically, mechanically, and histologically after 1, 2, and 4 weeks. Results: IP tendons showed improved healing compared with RP tendons. IP tendons were significantly stronger (32.2 N and 48.9 N, respectively) than RP tendons (20.1 N and 31.1 N, respectively) after 1 and 2 weeks. IP tendons did not elongate as much as RP tendons and had greater cross-sectional areas (18.0 mm2, 14.4 mm2, and 16.4 mm2, respectively) after 1, 2, and 4 weeks compared with RP tendons (10.5 mm2, 8.4 mm2, and 11.9 mm2, respectively). On histology, earlier collagen deposition and parallel orientation of fibrils were found for IP tendons. Conclusion: The paratenon is essential for efficient Achilles tendon healing. Healing with GFs in this Achilles tendon defect model was superior in the presence of the paratenon. Clinical Relevance: Biological approaches to tendon engineering using GFs are in vogue and have been shown to improve healing of the rat Achilles tendon, most likely by inducing progenitor cells located within the paratenon. Clinically, resection or incision of the paratenon has been proposed for wound closure. Our data demonstrate the fundamental importance of the paratenon, which therefore should be preserved during Achilles tendon repair, especially if augmented with products such as platelet-rich plasma or autologous conditioned serum that are rich in GFs.

Supplementation-time Dependence of Growth Factors in Promoting Tendon Healing

2006 ◽  
Vol 448 ◽  
pp. 240-247 ◽  
Author(s):  
B P Chan ◽  
S C Fu ◽  
L Qin ◽  
C Rolf ◽  
K M Chan
Keyword(s):  
Growth Factors ◽  
Time Dependence ◽  
Tendon Healing

Tendon Healing In Vivo: Gene Expression and Production of Multiple Growth Factors in Early Tendon Healing Period

2008 ◽  
Vol 33 (10) ◽  
pp. 1834-1842 ◽  
Author(s):  
Chuan Hao Chen ◽  
Yi Cao ◽  
Ya Fang Wu ◽  
Anthony J. Bais ◽  
Jing Song Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factors ◽  
Tendon Healing ◽  
Healing Period

scholarly journals Tendon Healing with Growth Factors

10.5772/32429 ◽  
2012 ◽  
Author(s):  
Sebastian Mller ◽  
Atanas Todorov ◽  
Patricia Heisterbach ◽  
Martin Majewski
Keyword(s):  
Growth Factors ◽  
Tendon Healing

Paper # 218: Tendon Healing: Modulation with Three Different Growth Factors

2011 ◽  
Vol 27 (10) ◽  
pp. e216
Author(s):  
Martin Majewski ◽  
Rudolf Flückiger ◽  
Christopher H. Evans
Keyword(s):  
Growth Factors ◽  
Tendon Healing

scholarly journals Optimizing growth factor induction of tenogenesis in three-dimensional culture of mesenchymal stem cells

2019 ◽  
Vol 10 ◽  
pp. 204173141984877 ◽  
Author(s):  
Ibtesam Rajpar ◽  
Jennifer G Barrett
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Tendon Healing ◽  
Collagen Type I ◽  
Marker Genes ◽  
Type I ◽  
Cell Alignment ◽  
Tgf Β1

Adult tissue stem cells have shown promise for the treatment of debilitating tendon injuries. However, few comparisons of stem cells from different tissue sources have been made to determine the optimum stem cell source for treating tendon. Moreover, it is likely that the application of tenogenic growth factors will improve tendon stem cell treatments further, and a comprehensive comparison of a number of growth factors is needed. Thus far, different types of stem cells cannot be evaluated in a high-throughput manner. To this end, we have developed an approach to culture mesenchymal stem cells isolated from bone marrow in collagen type I hydrogels with tenogenic growth factors using economical, commercially available supplies. To optimize growth factors for this assay, FGF-2, TGF-β1, IGF-1, and/or BMP-12 were tested singly and in novel combinations of (1) BMP-12 and IGF-1, (2) TGF-β1 and IGF-1, and/or (3) BMP-12 and FGF-2 over 10 days. Our data suggest that BMP-12 supplementation alone results in the strongest expression of tendon marker genes, controlled contractility of constructs, a higher degree of cell alignment, and tendon-like tissue morphology. This easy-to-use benchtop assay can be used to screen novel sources of stem cells and cell lines for tissue engineering and tendon healing applications.

Biologic Augmentation of Rotator Cuff Tendon-Healing with Use of a Mixture of Osteoinductive Growth Factors*

2007 ◽  
Vol 89 (11) ◽  
pp. 2485-2497 ◽  
Author(s):  
Scott A. Rodeo ◽  
Hollis G. Potter ◽  
Sumito Kawamura ◽  
A. Simon Turner ◽  
Hyon Jeong Kim ◽  
...  
Keyword(s):  
Growth Factors ◽  
Rotator Cuff ◽  
Tendon Healing ◽  
Rotator Cuff Tendon

Growth factors and canine flexor tendon healing: Initial studies in uninjured and repair models

1995 ◽  
Vol 20 (4) ◽  
pp. 645-649 ◽  
Author(s):  
Frederick J. Duffy ◽  
John G. Seiler ◽  
Richard H. Gelberman ◽  
Charles A. Hergrueter
Keyword(s):  
Growth Factors ◽  
Flexor Tendon ◽  
Tendon Healing ◽  
Repair Models

Biologic Augmentation of Rotator Cuff Tendon-Healing with Use of a Mixture of Osteoinductive Growth Factors*

2007 ◽  
Vol 89 (11) ◽  
pp. 2485-2497 ◽  
Author(s):  
Scott A. Rodeo ◽  
Hollis G. Potter ◽  
Sumito Kawamura ◽  
A. Simon Turner ◽  
Hyon Jeong Kim ◽  
...  
Keyword(s):  
Growth Factors ◽  
Rotator Cuff ◽  
Tendon Healing ◽  
Rotator Cuff Tendon
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