Staphylococcal Enterotoxins, Toxic Shock Syndrome Toxin and Streptococcal Pyrogenic Exotoxins: A Comparative Study of Their Molecular Biology (Part 1 of 2)

1992 ◽  
pp. 1-18 ◽  
Author(s):  
Marsha J. Betley ◽  
Deborah W. Borst ◽  
Laura B. Regassa
Keyword(s):  
Molecular Biology ◽  
Comparative Study ◽  
Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Staphylococcal Enterotoxins ◽  
Toxic Shock Syndrome Toxin

Staphylococcal Enterotoxins and Toxic Shock Syndrome Toxin-1 (TSST-1) Production During Induced Goat Mastitis

1991 ◽  
Vol 54 (4) ◽  
pp. 267-271 ◽  
Author(s):  
J. VALLE ◽  
E. GOMEZ-LUCIA ◽  
S. PIRIZ ◽  
S. VADILLO
Keyword(s):  
Toxic Shock Syndrome ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toxic Shock ◽  
Staphylococcal Enterotoxins ◽  
Milk Samples ◽  
Toxigenic Strains ◽  
Toxic Shock Syndrome Toxin ◽  
Type Strains

Six Staphylococcus aureus enterotoxigenic type strains were inoculated into the udders of healthy goats in order to check the “in vivo” production of staphylococcal enterotoxins and toxic shock syndrome toxin-one (TSST-1). Staphylococcal enterotoxins and TSST-1 produced by toxigenic strains in milk samples were detected by the enzyme-linked immunosorbent assay method. S. aureus strains FRI-100, FRI-1173, and CC-92 produced high levels of SEA, SEB, and TSST-1, respectively, in the inoculated goats' udders.

scholarly journals Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 734
Author(s):  
Wioleta Chajęcka-Wierzchowska ◽  
Joanna Gajewska ◽  
Patryk Wiśniewski ◽  
Anna Zadernowska
Keyword(s):  
Toxic Shock Syndrome ◽  
Food Poisoning ◽  
No Production ◽  
Toxic Shock ◽  
Coagulase Negative Staphylococci ◽  
Staphylococcal Enterotoxins ◽  
Food Borne ◽  
Enterotoxin Genes ◽  
Toxic Shock Syndrome Toxin ◽  
Coagulase Positive Staphylococci

Although coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role for the coagulase-negative staphylococci in the production of enterotoxins has been observed in recent years. This study was conducted to assess the occurrence of genes responsible for the production of staphylococcal enterotoxins (SE), enterotoxin-like toxins (SEI) and toxic shock syndrome toxin-1 (TSST-1) in coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food from bars and restaurants. One hundred and eighteen CoNS strains were tested using polymerase chain reaction (PCR) to five superantigenic toxin genes, including five different types of classical enterotoxins (sea, seb, sec, sed and see) and the toxic shock syndrome toxin-1 (tsst-1) as well as to supertoxin-like genes. PCR-positive isolates were then tested using immunoenzymatic methods (SET-RPLA, Vidas SET 2) for toxin expression. Out of 118 CoNS strains, the presence of staphylococcal enterotoxins was confirmed in 72% of them. The most frequently found enterotoxin-like genotype was ser, selu. Two of the tested strains had up to ten different enterotoxin genes in the genome at the same time. Although no production of enterotoxins was detected in the CoNS, which means that their possible role in the epidemiology of food-borne diseases is minimal, the data demonstrated that the toxigenic capacity of the CoNS should not be ignored, and that this group of microorganisms should be continuously monitored in food.

scholarly journals Use of Multiplex PCR To Detect Classical and Newly Described Pyrogenic Toxin Genes in Staphylococcal Isolates

1999 ◽  
Vol 37 (10) ◽  
pp. 3411-3414 ◽  
Author(s):  
Steven R. Monday ◽  
Gregory A. Bohach
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Toxic Shock Syndrome ◽  
Food Poisoning ◽  
Toxic Shock ◽  
Cellular Targets ◽  
Staphylococcal Enterotoxins ◽  
Toxin Genes ◽  
Toxic Shock Syndrome Toxin ◽  
Single Reaction

Staphylococcus aureus may contain one or more genes that encode a variety of immunomodulatory pyrogenic toxins (PTs), including the staphylococcal enterotoxins and toxic shock syndrome toxin (TSST). The PTs interact with several cellular targets to produce disease, such as food poisoning and toxic shock syndrome. At present, nine serologically distinct enterotoxins and one immunoreactive form of TSST have been identified and characterized. As isolates of S. aureus are further assessed, it is anticipated that this number will increase. To facilitate screening, a multiplex PCR was designed to simultaneously determine which of these 10 currently known PT genes an individualS. aureus isolate possesses. We show here, using S. aureus isolates with characterized PT phenotypes, that this novel PCR technique reliably detects each of the known PTs in a single reaction.

Sign in / Sign up

Export Citation Format

Share Document