scholarly journals Isolation and Functional Characterization of α-Smooth Muscle Actin Expressing Cardiomyocytes from Embryonic Stem Cells

2010 ◽  
Vol 25 (6) ◽  
pp. 595-604 ◽  
Author(s):  
Shiva Prasad Potta ◽  
Huamin Liang ◽  
Johannes Winkler ◽  
Michael Xavier Doss ◽  
Shuhua Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Embryonic Stem Cells ◽  
Smooth Muscle Actin ◽  
Functional Characterization ◽  
Embryonic Stem ◽  
Muscle Actin

scholarly journals Identification and Single-Cell Functional Characterization of an Endodermally Biased Pluripotent Substate in Human Embryonic Stem Cells

2018 ◽  
Vol 10 (6) ◽  
pp. 1895-1907 ◽  
Author(s):  
Thomas F. Allison ◽  
Andrew J.H. Smith ◽  
Konstantinos Anastassiadis ◽  
Jackie Sloane-Stanley ◽  
Veronica Biga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Functional Characterization ◽  
Embryonic Stem

scholarly journals Activation of Hes1 and Msx1 in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives

2018 ◽  
Vol 19 (12) ◽  
pp. 4025 ◽  
Author(s):  
Karla Méndez-Maldonado ◽  
Guillermo Vega-López ◽  
Sara Caballero-Chacón ◽  
Manuel Aybar ◽  
Iván Velasco
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Embryonic Stem Cells ◽  
Neural Crest ◽  
Nuclear Translocation ◽  
Smooth Muscle Actin ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Chimeric Proteins ◽  
Sox2 Expression

The neural crest (NC) comprises a multipotent cell population that produces peripheral neurons, cartilage, and smooth muscle cells, among other phenotypes. The participation of Hes1 and Msx1 when expressed in mouse embryonic stem cells (mESCs) undergoing NC differentiation is unexplored. In this work, we generated stable mESCs transfected with constructs encoding chimeric proteins in which the ligand binding domain of glucocorticoid receptor (GR), which is translocated to the nucleus by dexamethasone addition, is fused to either Hes1 (HGR) or Msx1 (MGR), as well as double-transgenic cells (HGR+MGR). These lines continued to express pluripotency markers. Upon NC differentiation, all lines exhibited significantly decreased Sox2 expression and upregulated Sox9, Snai1, and Msx1 expression, indicating NC commitment. Dexamethasone was added to induce nuclear translocation of the chimeric proteins. We found that Collagen IIa transcripts were increased in MGR cells, whereas coactivation of HGR+MGR caused a significant increase in Smooth muscle actin (α-Sma) transcripts. Immunostaining showed that activation in HGR+MGR cells induced higher proportions of β-TUBULIN III+, α-SMA+ and COL2A1+ cells. These findings indicate that nuclear translocation of MSX-1, alone or in combination with HES-1, produce chondrocyte-like cells, and simultaneous activation of HES-1 and MSX-1 increases the generation of smooth muscle and neuronal cells.

scholarly journals Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17771 ◽  
Author(s):  
Helena Vazão ◽  
Ricardo Pires das Neves ◽  
Mário Grãos ◽  
Lino Ferreira
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Embryonic Stem Cells ◽  
Smooth Muscle Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Muscle Cells

scholarly journals Functional characterization of B lymphocytes generated in vitro from embryonic stem cells

1999 ◽  
Vol 96 (17) ◽  
pp. 9797-9802 ◽  
Author(s):  
S. K. Cho ◽  
T. D. Webber ◽  
J. R. Carlyle ◽  
T. Nakano ◽  
S. M. Lewis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
B Lymphocytes ◽  
Functional Characterization ◽  
Embryonic Stem

Optimized Derivation and Functional Characterization of 5-HT Neurons from Human Embryonic Stem Cells

2009 ◽  
Vol 18 (4) ◽  
pp. 615-628 ◽  
Author(s):  
Manoj Kumar ◽  
Sanjeev K. Kaushalya ◽  
Pierre Gressens ◽  
Sudipta Maiti ◽  
Shyamala Mani
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Functional Characterization ◽  
Embryonic Stem

scholarly journals Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells

2020 ◽  
Vol Volume 12 ◽  
pp. 15-27 ◽  
Author(s):  
Rajagopal N Aravalli ◽  
Daniel Collins ◽  
Joel Hapke ◽  
Andrew Crane ◽  
Clifford J Steer
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Functional Characterization ◽  
Embryonic Stem ◽  
Hepatic Differentiation
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