Halothane Anesthesia in Mice: Effect on the Phagocytic Activity and Respiratory Burst of Peritoneal Macrophages

2011 ◽  
Vol 18 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Darío Colucci ◽  
Guillermina Harvey ◽  
María Cristina Gayol ◽  
Gustavo Elena ◽  
Nora Puig
Keyword(s):  
Respiratory Burst ◽  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Halothane Anesthesia

Effect of praziquantel and liposome-incorporated praziquantel on peritoneal macrophage activation in mice infected with Mesocestoides corti tetrathyridia (Cestoda)

Parasitology ◽  
1997 ◽  
Vol 114 (5) ◽  
pp. 475-482 ◽  
Author(s):  
G. HRČKOVA ◽  
S. VELEBNY
Keyword(s):  
Peritoneal Macrophage ◽  
Peritoneal Cavity ◽  
Respiratory Burst ◽  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Drug Formulation ◽  
Superoxide Anions ◽  
Effector Functions ◽  
Mouse Model System

The activation of peritoneal macrophage effector functions after therapy with free PZQ and PZQ incorporated in liposomes (lip.PZQ) was studied in the Mesocestoides corti–mouse model system. Each drug formulation was administered to an infected group of mice in 6 daily doses from day 14 p.i. Phagocytic activity of macrophages increased significantly after the administration of both drug formulations, more after lip.PZQ with an earlier peak observed for PZQ (day 3) than for lip.PZQ (day 6). Empty liposomes had no significant effect. The average counts of ingested particles in phagocytosing cells were significantly higher only after lip.PZQ administration. The pattern of changes in phagocytic activity correlated with the reduction of parasite numbers in the peritoneal cavity, with the highest observed on day 6 after therapy with lip.PZQ. Phagocytosis of lip.PZQ in vivo stimulated significantly the respiratory burst in peritoneal macrophages, with the highest concentration of superoxide anions recorded on day 1 after the last dose, whereas therapy with PZQ itself did not increase this process significantly. The capacity for the respiratory burst declined in all groups with progressing infection. It is proposed that the phagocytic activity of peritoneal macrophages after therapy was stimulated indirectly as a consequence of activation of the specific immune response. The larvicidal effect of lip.PZQ on the tetrathyridia in the peritoneal cavity was synergistic with the phagocytic activity and might be the result of double action of drug and superoxide anions generated during the respiratory burst stimulated by this drug formulation.

Trichilia glabra: effect on the phagocytic activity and respiratory burst response of peritoneal macrophages

1999 ◽  
Vol 41 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Fabián Benencia ◽  
Marı́a Cecilia Courrèges ◽  
Félix C Coulombié
Keyword(s):  
Respiratory Burst ◽  
Phagocytic Activity ◽  
Peritoneal Macrophages

scholarly journals Effects of geraniin on the morphology and phagocytic activity of mouse peritoneal macrophages

1989 ◽  
Vol 49 ◽  
pp. 265
Author(s):  
Tang Fang ◽  
Yumiko Ushio ◽  
Hiroyoshi Konishi ◽  
Takuo Okuda ◽  
Hiroko Abe
Keyword(s):  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages

scholarly journals Phagocytic activity of blood monocytes in response to methicillin-resistant strains of Staphylococcus aureus

2020 ◽  
Vol 10 (3) ◽  
pp. 551-557
Author(s):  
O. A. Kolenchukova ◽  
N. I. Sarmatova ◽  
A. V. Moshev
Keyword(s):  
Respiratory Burst ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Fluorescein Isothiocyanate ◽  
Phagocytic Index ◽  
Blood Monocytes ◽  
Monocyte Subsets ◽  
Superoxide Anion Production ◽  
Hla Dr ◽  
Burst Intensity

Current study performed to estimate the phagocytic activity of blood monocytes of varying phenotypes exposed to MRSA and MSSA strains.  Objects: Blood monocytes were collected from 25 healthy adults (age: 25–45 years). Live suspensions of MRSA/MSSA strains were used at concentration of 106 colony-forming units (CFU)/mL.  Metods. Phagocytic functions were estimated by using fluorescein isothiocyanate (FITC)-labelled MRSA and MSSA strains followed by running flow cytometry on FC 500 series flow cytometer (Beckman Coulter, USA). Whole peripheral blood cells were directly labelled with immunofluorescently tagged monoclonal CD14-PE/CD45-ECD/HLA-DR-PC5/CD16-PC7 antibodies (Beckman Coulter, USA). Respiratory burst intensity was evaluated in monocytes by measuring activity of lucigeninand luminol-dependent spontaneous and induced chemiluminescence. Monocytes were induced by using live suspension of MRSA/MSSA strains at a concentration of 106 CFU/mL. Results and discussion. While studying luminol-dependent monocyte activities after exposure to MRSA vs. MSSA, it was observed a 3.5-fold decreased curve square, whereas lucigenin-dependent chemiluminescence was increased by 6-fold. Compared to MSSA exposure, index of activation (IA) was decreased by 1.1-fold in response to MRSA exposure that was confirmed by lowered release of reactive oxygen species (ROS) from monocytes in response to MRSA exposure. Moreover, IRSS increased by 1.3-fold upon MRSA exposure. Examining monocyte oxygen-independent phagocytosis against MRSA vs. MSSA revealed significantly increased phagocytic number and concomitantly decreased phagocytic index. An evaluation of the activities of various monocyte subsets in response to MRSA vs. MSSA revealed increased phagocytic index by 1.5-fold for CD14lowCD16+ and CD14+CD16+ monocyte subsets as well as 3-fold for CD14+CD16– monocytes. Counts for all phagocytic subsets were decreased (1.4-, 1.5- and 4-fold for CD14lowCD16+, CD14+CD16+ and CD14+CD16– monocytes, respectively). To summarize, intensity of the respiratory burst was lowered upon MRSA exposure and percentage of monocyte subsets. Overall deficiency of superoxide anion production was observed in response to MRSA. In contrast, oxygen-independent event revealed phenotypic changes in frequency of peripheral blood monocytes upon MRSA exposure. We observed that CD14+CD16– classical monocytes were more rapidly activated. Conclusion. Thus, we concluded that CD14+CD16– monocytes became more rapidly activated but exhibited less effective phagocytosis, whereas CD14+CD16+ and CD14lowCD16+ monocytes were more slowly activated and demonstrated stronger phagocytic activity.

Assessment of the immunomodulatory effect of Aloe vera polysaccharides extracts on macrophages functions

2020 ◽  
pp. 408
Author(s):  
Berenice Aranda-Cuevas ◽  
Jorge Tamayo- Cortez ◽  
Lourdes Vargas y Vargas ◽  
Ignacio Islas- Flores ◽  
Víctor Arana- Argáez ◽  
...  
Keyword(s):  
Cell Viability ◽  
Phagocytic Activity ◽  
Rainy Season ◽  
Aloe Vera ◽  
Peritoneal Macrophages ◽  
Dry Season ◽  
Immunomodulatory Effect ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Proliferative Effect

The present study evaluates the immunomodulatory effect of high molecular weight fractions of Aloe vera polysaccharides harvested during the dry season (March-April) and the rainy season (August-September). Peritoneal macrophages (MΦs) secluded from Balb/c mice underwent treatment with A. vera leaves extract and acemannan standard (the major component found in A. vera) and stimulated with lipopolysaccharides (LPS). Macrophage cell viability was assessed by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Phagocytic activity was also evaluated in peritoneal macrophages, such as the production of nitric oxide and interleukin 6 (IL-6). In the results, found that the A. vera polysaccharides harvested during the rainy season stimulated the phagocytic activity with greater intensity than dry season and improvement NO and IL-6 production. No cytotoxic effect was found on cell viability and they cause a significant proliferative effect on macrophages in a concentration-dependent manner. It can be concluded that the A. vera polysaccharides harvested during the rainy season possessed a stronger immunostimulatory effect compared to the extracts from leaves obtained during dry seasons in a concentration-dependent manner without aff at the cell viability of macrophages.

Effect of sodium thiopentone anesthesia on the phagocytic activity of rat peritoneal macrophages

Life Sciences ◽  
1998 ◽  
Vol 63 (25) ◽  
pp. 2221-2226 ◽  
Author(s):  
Hertzel Salman ◽  
Michael Bergman ◽  
Hanna Bessler ◽  
Svetlana Alexandrova ◽  
Benzion Beilin ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
Peritoneal Macrophages

Effect of carotenoids on the respiratory burst of rat peritoneal macrophages

1998 ◽  
Vol 1381 (1) ◽  
pp. 77-88 ◽  
Author(s):  
Wen'en Zhao ◽  
Yashan Han ◽  
Baolu Zhao ◽  
Saishi Hirota ◽  
Jingwu Hou ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Peritoneal Macrophages

Immunoprotective Effect of Cu/Zn Superoxide Dismutase on Myeloid Graffi Tumor-Bearing Hamsters

2000 ◽  
Vol 55 (7-8) ◽  
pp. 649-656 ◽  
Author(s):  
Reneta A. Toshkova ◽  
Petia A. Dimitrova ◽  
Emilia H. Ivanova ◽  
Pavlina A. Dolashka ◽  
Maria B. Angelova ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Tumor Growth ◽  
Protective Effect ◽  
Phagocytic Activity ◽  
Tumor Antigens ◽  
Early Stage ◽  
Tumor Development ◽  
Peritoneal Macrophages ◽  
Tumor Bearing

Abstract Investigation on the immunoprotective activity of Cu/Zn superoxide dismutase from Humicola lutea 103 AL (HLSOD) in hamsters with transplanted myeloid tumor was performed. Survivability, tumor growth and tumor transplantability were followed. The immune status of tumor-bearing animals, injected with the optimal protective HLSOD dose, was examined during 27 days after tumor transplantation by the following parameters: (i) the number, migration and phagocytic activity of peritoneal macrophages, (ii) the phagocytic activity of blood polymorphonuclear leukocytes (PMNs), (iii) the responsibility in vitro of spleen lymphocytes to T and B cell mitogens. It was established that intraperitoneal inoculation of HLSOD produced a protective effect on the development of tumors. Elongation of the latent time for tumor appearance and inhibition of the tumor growth were observed. The decreased percentage of mortality in early stage of tumor progression was established. Immunological studies on tumor-bearing hamsters (TBH) induced a tem porary immunorestoring effect on the suppressed phagocytic activities of peritoneal macrophages and blood PMNs during the first 14 days of tumor development. Moreover, HLSOD showed an expressed stimulating effect on proliferative activity in vitro of spleen B lymphocytes from healthy and TBH as well. The immunorestoring and protective effect of the enzyme was probably due to improve of the oxidant-antioxidant balance in peritoneal phagocytes. The tem porary character of the effect can be explained with the interference of immunosuppressing factors produced by tumor tissue as well as by the presence of tumor antigens, tumor cells and antigen-antibody complexes in the circulation.

Methyl palmitate inhibits lipopolysaccharide-stimulated phagocytic activity of rat peritoneal macrophages

2006 ◽  
Vol 20 (6) ◽  
pp. 302-308 ◽  
Author(s):  
Swapna Sarkar ◽  
M. Firoze Khan ◽  
Bhupendra S. Kaphalia ◽  
G. A. S. Ansari
Keyword(s):  
Phagocytic Activity ◽  
Methyl Palmitate ◽  
Peritoneal Macrophages
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