Methyl palmitate inhibits lipopolysaccharide-stimulated phagocytic activity of rat peritoneal macrophages

2006 ◽  
Vol 20 (6) ◽  
pp. 302-308 ◽  
Author(s):  
Swapna Sarkar ◽  
M. Firoze Khan ◽  
Bhupendra S. Kaphalia ◽  
G. A. S. Ansari
Keyword(s):  
Phagocytic Activity ◽  
Methyl Palmitate ◽  
Peritoneal Macrophages

scholarly journals Effects of geraniin on the morphology and phagocytic activity of mouse peritoneal macrophages

1989 ◽  
Vol 49 ◽  
pp. 265
Author(s):  
Tang Fang ◽  
Yumiko Ushio ◽  
Hiroyoshi Konishi ◽  
Takuo Okuda ◽  
Hiroko Abe
Keyword(s):  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages

Assessment of the immunomodulatory effect of Aloe vera polysaccharides extracts on macrophages functions

2020 ◽  
pp. 408
Author(s):  
Berenice Aranda-Cuevas ◽  
Jorge Tamayo- Cortez ◽  
Lourdes Vargas y Vargas ◽  
Ignacio Islas- Flores ◽  
Víctor Arana- Argáez ◽  
...  
Keyword(s):  
Cell Viability ◽  
Phagocytic Activity ◽  
Rainy Season ◽  
Aloe Vera ◽  
Peritoneal Macrophages ◽  
Dry Season ◽  
Immunomodulatory Effect ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Proliferative Effect

The present study evaluates the immunomodulatory effect of high molecular weight fractions of Aloe vera polysaccharides harvested during the dry season (March-April) and the rainy season (August-September). Peritoneal macrophages (MΦs) secluded from Balb/c mice underwent treatment with A. vera leaves extract and acemannan standard (the major component found in A. vera) and stimulated with lipopolysaccharides (LPS). Macrophage cell viability was assessed by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Phagocytic activity was also evaluated in peritoneal macrophages, such as the production of nitric oxide and interleukin 6 (IL-6). In the results, found that the A. vera polysaccharides harvested during the rainy season stimulated the phagocytic activity with greater intensity than dry season and improvement NO and IL-6 production. No cytotoxic effect was found on cell viability and they cause a significant proliferative effect on macrophages in a concentration-dependent manner. It can be concluded that the A. vera polysaccharides harvested during the rainy season possessed a stronger immunostimulatory effect compared to the extracts from leaves obtained during dry seasons in a concentration-dependent manner without aff at the cell viability of macrophages.

Effect of sodium thiopentone anesthesia on the phagocytic activity of rat peritoneal macrophages

Life Sciences ◽  
1998 ◽  
Vol 63 (25) ◽  
pp. 2221-2226 ◽  
Author(s):  
Hertzel Salman ◽  
Michael Bergman ◽  
Hanna Bessler ◽  
Svetlana Alexandrova ◽  
Benzion Beilin ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
Peritoneal Macrophages

Immunoprotective Effect of Cu/Zn Superoxide Dismutase on Myeloid Graffi Tumor-Bearing Hamsters

2000 ◽  
Vol 55 (7-8) ◽  
pp. 649-656 ◽  
Author(s):  
Reneta A. Toshkova ◽  
Petia A. Dimitrova ◽  
Emilia H. Ivanova ◽  
Pavlina A. Dolashka ◽  
Maria B. Angelova ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Tumor Growth ◽  
Protective Effect ◽  
Phagocytic Activity ◽  
Tumor Antigens ◽  
Early Stage ◽  
Tumor Development ◽  
Peritoneal Macrophages ◽  
Tumor Bearing

Abstract Investigation on the immunoprotective activity of Cu/Zn superoxide dismutase from Humicola lutea 103 AL (HLSOD) in hamsters with transplanted myeloid tumor was performed. Survivability, tumor growth and tumor transplantability were followed. The immune status of tumor-bearing animals, injected with the optimal protective HLSOD dose, was examined during 27 days after tumor transplantation by the following parameters: (i) the number, migration and phagocytic activity of peritoneal macrophages, (ii) the phagocytic activity of blood polymorphonuclear leukocytes (PMNs), (iii) the responsibility in vitro of spleen lymphocytes to T and B cell mitogens. It was established that intraperitoneal inoculation of HLSOD produced a protective effect on the development of tumors. Elongation of the latent time for tumor appearance and inhibition of the tumor growth were observed. The decreased percentage of mortality in early stage of tumor progression was established. Immunological studies on tumor-bearing hamsters (TBH) induced a tem porary immunorestoring effect on the suppressed phagocytic activities of peritoneal macrophages and blood PMNs during the first 14 days of tumor development. Moreover, HLSOD showed an expressed stimulating effect on proliferative activity in vitro of spleen B lymphocytes from healthy and TBH as well. The immunorestoring and protective effect of the enzyme was probably due to improve of the oxidant-antioxidant balance in peritoneal phagocytes. The tem porary character of the effect can be explained with the interference of immunosuppressing factors produced by tumor tissue as well as by the presence of tumor antigens, tumor cells and antigen-antibody complexes in the circulation.

scholarly journals Immunomodulatory Activities of Dendrophthoe falcata (L.f) Ettingsh in Experimental Animals: In vitro and In vivo Investigations

2011 ◽  
Vol 3 (3) ◽  
pp. 619-630 ◽  
Author(s):  
S. P. Pattanayak ◽  
P. M. Mazumder
Keyword(s):  
Antibody Titer ◽  
Phagocytic Activity ◽  
Reticuloendothelial System ◽  
Peritoneal Macrophages ◽  
Immunomodulatory Activity ◽  
Hydroalcoholic Extract ◽  
Dendrophthoe Falcata ◽  
Stimulation Of

In the present study, an attempt was made to screen immunomodulatory activity of the hydroalcoholic extract (HEDF) of Dendrophthoe falcata (L.f.) Ettingsh (Loranthaceae), an Indian Ayurvedic plant, on different arms of the immune system. HEDF was evaluated for immunological function by studying delayed type hypersensitivity (DTH) to sheep RBCs, nitric oxide (NO) release from murine peritoneal macrophages, phagocytic activity of polymorphonuclear (PMN) cells in vitro and reticuloendothelial system in vivo, plaque forming cell response of splenic lymphocytes to sheep erythrocytes, haemagglutination antibody titer and neutrophil adhesion test. Significant increase in NO production by mouse peritoneal macrophages was detected in culture supernatants indicated increased phagocytic activity of macrophages. After post oral administration of HEDF in three doses of 250, 475 and 950 mg/kg body weight, a significant increase in phagocytic activity of PMN cells/reticuloendothelial system, stimulation of neutrophil function and splenic antibody secreting cells, were also noticed. Stimulation of humoral immune response was further observed with elevation in haemagglutination antibody titer. Heightened DTH reaction suggested convincing evidence for activation of cellular immune system. Present study thus confirms the immunomodulatory activity of the hydroalcoholic extract of D. falcata and the immunomodulatory responses were found to be dose dependent manner.Keywords: Dendrophthoe falcata; Antibody titer; Neutrophil adhesion; Phagocytic activity.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i3.7655               J. Sci. Res. 3 (3), 629-640 (2011)

scholarly journals Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory

2017 ◽  
Vol 21 (3) ◽  
pp. 182
Author(s):  
Kusmiati . ◽  
Fifi Afiati ◽  
Farha Elein Kukihi
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Lactobacillus Delbrueckii ◽  
Immunomodulatory Activity ◽  
Phagocytic Capacity ◽  
Macrophage Cells ◽  
Ft Ir

<p>Immunomodulatory activity assay and characterization of exopolysaccharide (EPS) from Lactic Acid Bacteria (LAB) was done in Bogor. Bacteria used in this study was LAB strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Exopolysaccharide was extracted from L. delbrueckii subsp. bulgaricus and S. thermophilus then characterized with FT-IR spectrophotometer to determine the functional group. IR spectrum analysis using Fourier Transform-Infra Red (FT-IR) showed that EPS from both LAB isolates were carbohydrate compounds. Immunomodulatory activity in vivo from EPS was measured using phagocytic activity and phagocytic capacity macrophage cells from mice peritoneal cavity fluid. Exopolysaccharide were given orally to mice in concentrations of 100 μg/ml, 200 μg/ml and 300 μg/ml for 14 days then the mice were infected with Staphylococcus aureus. Result showed that EPS from both LAB isolate enhanced either phagocytic activity and phagocytic capacity macrophage cell from mice peritoneal fluid. EPS from L. delbrueckii subsp. bulgaricus concentration 300 μg/ml showed the highest phagocytic activity of macrophage cells and EPS from S. thermophilus concentration 300 μg/ml showed the highest phagocytic capacity. It is concluded that EPS potency tested as immunomodulatory derived from a culture of L. delbrueckii and S. thermophilus subsp.bulgaricus are able to increase the activity and phagocytosis murine peritoneal macrophages.</p>

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