scholarly journals AnEscherichia coli-Based Cell-Free System for Large-Scale Production of Functional Mammalian Membrane Proteins Suitable for X-Ray Crystallography

2010 ◽  
Vol 18 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Tuan A. Nguyen ◽  
Samantha S. Lieu ◽  
Geoffrey Chang
Keyword(s):  
Membrane Proteins ◽  
Large Scale ◽  
Scale Production ◽  
Free System ◽  
X Ray ◽  
Cell Free System ◽  
X Ray Crystallography ◽  
Large Scale Production

scholarly journals Heterologous Expression and Purification Systems for Structural Proteomics of Mammalian Membrane Proteins

2002 ◽  
Vol 3 (6) ◽  
pp. 511-517 ◽  
Author(s):  
Isabelle Mus-Veteau
Keyword(s):  
Membrane Proteins ◽  
Heterologous Expression ◽  
Large Scale ◽  
Structural Information ◽  
Structural Data ◽  
Structural Proteomics ◽  
Scale Production ◽  
Expression And Purification ◽  
Large Scale Production ◽  
Heterologous Expression Systems

Membrane proteins (MPs) are responsible for the interface between the exterior and the interior of the cell. These proteins are implicated in numerous diseases, such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, heart failure, hypertension and Alzheimer's disease. However, studies on these disorders are hampered by a lack of structural information about the proteins involved. Structural analysis requires large quantities of pure and active proteins. The majority of medically and pharmaceutically relevant MPs are present in tissues at very low concentration, which makes heterologous expression in large-scale production-adapted cells a prerequisite for structural studies. Obtaining mammalian MP structural data depends on the development of methods that allow the production of large quantities of MPs. This review focuses on the different heterologous expression systems, and the purification strategies, used to produce large amounts of pure mammalian MPs for structural proteomics.

Variables Involved in the Planting of Rice in the Rice Husk

2012 ◽  
Vol 517 ◽  
pp. 430-436
Author(s):  
J.L. Akasaki ◽  
E.J. Silva ◽  
L.C. Sousa ◽  
J.L.P. Melges ◽  
M.M. Tashima ◽  
...  
Keyword(s):  
Rice Husk ◽  
Large Scale ◽  
Rice Husk Ash ◽  
Scale Production ◽  
X Ray Diffraction ◽  
X Ray ◽  
Large Scale Production ◽  
Pozzolanic Materials ◽  
Different Strains ◽  
Different Origins

Rice production in Brazil in 2010 was approximately 12,2 million tons, and to reach that amount several planting techniques were used with different strains of rice in different rice-growing areas. Since Rice Husk Ash (RHA) is the pozzolan of vegetable origin most studied by researchers working in the area of pozzolanic materials, the present paper evaluates the influence that the form of planting, the climate, the soil, the strain of rice and the origin/amount of nitrogen-based fertilizers used in rice cultivation has on the chemical composition and crystallographic properties of RHA. The results obtained in this paper, confirm the importance of carrying out routine chemical analysis and X-Ray diffraction to maintain the quality control of the CCAs produced, because in situations of large-scale production, husks of different origins may be used.

scholarly journals SPINE-compatible `carboloops': a new microshaped vitreous carbon sample mount for X-ray and neutron crystallography

2014 ◽  
Vol 70 (5) ◽  
pp. 681-684 ◽  
Author(s):  
Filippo Romoli ◽  
Estelle Mossou ◽  
Maxime Cuypers ◽  
Peter van der Linden ◽  
Philippe Carpentier ◽  
...  
Keyword(s):  
Large Scale ◽  
Production Quality ◽  
Vitreous Carbon ◽  
Scale Production ◽  
X Rays ◽  
Macromolecular Crystallography ◽  
X Ray ◽  
Large Scale Production ◽  
Custom Design ◽  
High Standards

A novel vitreous carbon mount for macromolecular crystallography, suitable for neutron and X-ray crystallographic studies, has been developed. The technology described here is compatible both with X-ray and neutron cryo-crystallography. The mounts have low density and low background scattering for both neutrons and X-rays. They are prepared by laser cutting, allowing high standards of production quality, the ability to custom-design the mount to specific crystal sizes and large-scale production.

Programmatics of large scale production of silicon pore optics for future x-ray telescopes

2006 ◽  
Author(s):  
S. Kraft ◽  
M. Collon ◽  
M. W. Beijersbergen ◽  
M. Bavdaz ◽  
D. H. Lumb ◽  
...  
Keyword(s):  
Large Scale ◽  
Scale Production ◽  
X Ray ◽  
Large Scale Production

Large-scale production of functional membrane proteins

2008 ◽  
Vol 65 (11) ◽  
pp. 1729-1755 ◽  
Author(s):  
F. Junge ◽  
B. Schneider ◽  
S. Reckel ◽  
D. Schwarz ◽  
V. Dötsch ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production ◽  
Functional Membrane

A New Synthetic Route to Nano Metal Oxides

2011 ◽  
Vol 675-677 ◽  
pp. 223-226
Author(s):  
Hong Zhao ◽  
Jin Hua Yao ◽  
Shu Bao Jin ◽  
Li Jun Liang
Keyword(s):  
Metal Oxides ◽  
Large Scale ◽  
Mixed Oxides ◽  
Scale Production ◽  
X Ray Diffraction ◽  
Mechanical Grinding ◽  
Energy Dispersion Spectroscopy ◽  
X Ray ◽  
Large Scale Production ◽  
Key Steps

Strong alkali anion exchange resin was employed as a hydroxyl source in the synthesis. OH- releasing rates were easily controlled by varying precursor salts, salt concentrations and temperatures, etc. The ratio between the nucleation speed and the speed of oxide crystallite growth was adjusted to favor the anisotropic growth of the crystal. Zinc and aluminum nano-sized oxide were obtained in various morphologies. X-ray diffraction (XRD), scanning electron microscope (SEM) and Energy Dispersion Spectroscopy (EDS) were employed to characterize the products. It is ascertained that the surface reaction on the resin and the mechanical grinding are the key steps in controlling the product morphology. The results show the flexibility and possibility of large scale production of various nano metal oxides and as well as mixed oxides.

scholarly journals Gold nano-mesh synthesis by continuous-flow X-ray irradiation

2019 ◽  
Vol 26 (6) ◽  
pp. 1929-1935 ◽  
Author(s):  
Min-Tsang Li ◽  
Sheng-Feng Lai ◽  
Shun-Min Yang ◽  
Yu-Sheng Chen ◽  
Ying-Jie Chen ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Irradiation Dose ◽  
Large Scale ◽  
Precursor Solution ◽  
Scale Production ◽  
X Ray ◽  
Precise Control ◽  
Synthesis Parameters ◽  
Large Scale Production

X-ray irradiation has been extensively used in recent years as a fabrication step for nanoparticles and nanoparticle systems. A variant of this technique, continuous-flow X-ray irradiation, has recently been developed, and offers three important advantages: precise control of the irradiation dose, elimination of convection effects in the precursor solution, and suitability for large-scale production. Here, the use of this method to fabricate Au nano-meshes of interest as transparent and flexible electrodes for optoelectronics is reported. The study includes extensive characterization of the synthesis parameters and of the product properties, with rather encouraging results.

Sign in / Sign up

Export Citation Format

Share Document