Unusual Chromosomal Distribution of a Major Satellite DNA from Discoglossus pictus (Amphibia, Anura)

2009 ◽  
Vol 127 (1) ◽  
pp. 33-42 ◽  
Author(s):  
N. Amor ◽  
G. Odierna ◽  
G. Chinali ◽  
K. Said ◽  
O. Picariello
Keyword(s):  
Satellite Dna ◽  
Chromosomal Distribution ◽  
Major Satellite ◽  
Discoglossus Pictus

Chromosomal distribution of the major satellite DNA of South American rodents of the genus Ctenomys

1995 ◽  
Vol 69 (3-4) ◽  
pp. 179-184 ◽  
Author(s):  
M.S. Rossi ◽  
C.A. Redi ◽  
G. Viale ◽  
A.I. Massarini ◽  
E. Capanna
Keyword(s):  
Satellite Dna ◽  
South American ◽  
Chromosomal Distribution ◽  
Major Satellite

Dissociation of minor satellite from the centromere in mouse

1994 ◽  
Vol 107 (11) ◽  
pp. 3091-3095
Author(s):  
B.K. Vig ◽  
D. Latour ◽  
J. Frankovich
Keyword(s):  
Satellite Dna ◽  
Hoechst 33258 ◽  
Major Satellite ◽  
Primary Constriction ◽  
Minor Satellite ◽  
Antibody Reaction

The minor satellite DNA of mouse is believed to constitute the centromere. We report that centromeres of some chromosomes in the Cl1D cells of mouse are not associated with this DNA even though the latter is present on these chromosomes. The satellite DNA was detected distally from the centromere and could not be mistaken as a component of the centromere. We also report that the site of the primary constriction may not always coincide with the site of the anti-kinetochore antibody reaction. Whereas the regions containing the major satellite decondense upon treatment with bisbenzimidazole (Hoechst 33258), the sites carrying minor satellite resist decondensing.

Chromatin organization in the homogeneously staining regions of a methotrexate-resistant mouse cell line: interspersion of inactive and active chromatin domains distinguished by acetylation of histone H4

1996 ◽  
Vol 109 (9) ◽  
pp. 2221-2228 ◽  
Author(s):  
L. Nicol ◽  
P. Jeppesen
Keyword(s):  
Cell Line ◽  
Dna Sequences ◽  
Satellite Dna ◽  
Mouse Cell ◽  
Histone H4 ◽  
Active Chromatin ◽  
Chromatin Domains ◽  
Resistant Mouse ◽  
Major Satellite ◽  
Drosophila Heterochromatin

We have analyzed the organization of the homogeneously staining regions (HSRs) in chromosomes from a methotrexate-resistant mouse melanoma cell line. Fluorescence in situ hybridization techniques were used to localize satellite DNA sequences and the amplified copies of the dihydrofolate reductase (DHFR) gene that confer drug-resistance, in combination with immunofluorescence using antibody probes to differentiate chromatin structure. We show that the major DNA species contained in the HSRs is mouse major satellite, confirming previous reports, and that this is interspersed with DHFR DNA in an alternating tandem array that can be resolved at the cytological level. Mouse minor satellite DNA, which is normally located at centromeres, is also distributed along the HSRs, but does not appear to interfere with centromere function. The blocks of major satellite DNA are coincident with chromatin domains that are labelled by an autoantibody that recognizes a mammalian homologue of Drosophila heterochromatin-associated protein 1, shown previously to be confined to centric heterochromatin in mouse. An antiserum that specifically recognizes acetylated histone H4, a marker for active chromatin, fails to bind to the satellite DNA domains, but labels the intervening segments containing DHFR DNA. We can find no evidence for the spreading of the inactive chromatin domains into adjacent active chromatin, even after extended passaging of cells in the absence of methotrexate selection.

scholarly journals Biogeographic patterns in the chromosomal distribution of a satellite DNA in the banded tetra Astyanax fasciatus (Teleostei: Characiformes)

2012 ◽  
Vol 13 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Karine Frehner Kavalco ◽  
Rubens Pazza ◽  
Karina de Oliveira Brandão ◽  
Lurdes Foresti de Almeida-Toledo
Keyword(s):  
Satellite Dna ◽  
Chromosomal Distribution ◽  
Biogeographic Patterns ◽  
Astyanax Fasciatus

scholarly journals Sequence Analysis and FISH Mapping of Four Satellite DNA Families among Cervidae

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 584
Author(s):  
Miluse Vozdova ◽  
Svatava Kubickova ◽  
Halina Cernohorska ◽  
Jan Fröhlich ◽  
Natália Martínková ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Satellite Dna ◽  
Phylogenetic Trees ◽  
In Situ Hybridisation ◽  
Gc Content ◽  
Copy Number Variations ◽  
Binding Motif ◽  
Fluorescence In Situ Hybridisation ◽  
Chromosomal Distribution ◽  
Satellite Dnas

Centromeric and pericentromeric chromosome regions are occupied by satellite DNA. Satellite DNAs play essential roles in chromosome segregation, and, thanks to their extensive sequence variability, to some extent, they can also be used as phylogenetic markers. In this paper, we isolated and sequenced satellite DNA I-IV in 11 species of Cervidae. The obtained satellite DNA sequences and their chromosomal distribution were compared among the analysed representatives of cervid subfamilies Cervinae and Capreolinae. Only satI and satII sequences are probably present in all analysed species with high abundance. On the other hand, fluorescence in situ hybridisation (FISH) with satIII and satIV probes showed signals only in a part of the analysed species, indicating interspecies copy number variations. Several indices, including FISH patterns, the high guanine and cytosine (GC) content, and the presence of centromere protein B (CENP-B) binding motif, suggest that the satII DNA may represent the most important satellite DNA family that might be involved in the centromeric function in Cervidae. The absence or low intensity of satellite DNA FISH signals on biarmed chromosomes probably reflects the evolutionary reduction of heterochromatin following the formation of chromosome fusions. The phylogenetic trees constructed on the basis of the satellite I-IV DNA relationships generally support the present cervid taxonomy.

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