The chromosomal distribution of satellite DNA in the germ-line and somatic tissues of the gall midge, Heteropeza pygmaea

Chromosoma ◽  
1974 ◽  
Vol 47 (1) ◽  
pp. 1-19 ◽  
Author(s):  
Werner Kunz ◽  
Ronald A. Eckhardt
Keyword(s):  
Satellite Dna ◽  
Germ Line ◽  
Gall Midge ◽  
Chromosomal Distribution ◽  
Somatic Tissues ◽  
Heteropeza Pygmaea

scholarly journals Nucleotide sequences of highly repeated DNAs; compilation and comments

1982 ◽  
Vol 39 (1) ◽  
pp. 1-30 ◽  
Author(s):  
George L. Gabor Miklos ◽  
Amanda Clare Gill
Keyword(s):  
Satellite Dna ◽  
Germ Line ◽  
Sequence Data ◽  
Allelic Variation ◽  
Neutral Theory ◽  
Natural Populations ◽  
Evolutionary Significance ◽  
Nucleotide Sequence Data ◽  
A Genome ◽  
Repeated Dnas

SummaryThe nucleotide sequence data from highly repeated DNAs of inverte-brates and mammals are summarized and briefly discussed. Very similar conclusions can be drawn from the two data bases. Sequence complexities can vary from 2 bp to at least 359 bp in invertebrates and from 3 bp to at least 2350 bp in mammals. The larger sequences may or may not exhibit a substructure. Significant sequence variation occurs for any given repeated array within a species, but the sources of this heterogeneity have not been systematically partitioned. The types of alterations in a basic repeating unit can involve base changes as well as deletions or additions which can vary from 1 bp to at least 98 bp in length. These changes indicate that sequence per se is unlikely to be under significant biological constraints and may sensibly be examined by analogy to Kimura's neutral theory for allelic variation. It is not possible with the present evidence to discriminate between the roles of neutral and selective mechanisms in the evolution of highly repeated DNA.Tandemly repeated arrays are constantly subjected to cycles of amplification and deletion by mechanisms for which the available data stem largely from ribosomal genes. It is a matter of conjecture whether the solutions to the mechanistic puzzles involved in amplification or rapid redeployment of satellite sequences throughout a genome will necessarily give any insight into biological functions.The lack of significant somatic effects when the satellite DNA content of a genome is significantly perturbed indicates that the hunt for specific functions at the cellular level is unlikely to prove profitable.The presence or in some cases the amount of satellite DNA on a chromosome, however, can have significant effects in the germ line. There the data show that localized condensed chromatin, rich in satellite DNA, can have the effect of rendering adjacent euchromatic regions rec−, or of altering levels of recombination on different chromosomes. No data stemming from natural populations however are yet available to tell us if these effects are of adaptive or evolutionary significance.

Sex determination in the germ line of Drosophila melanogaster: activation of the gene Sex-lethal

Development ◽  
1993 ◽  
Vol 118 (3) ◽  
pp. 813-816 ◽  
Author(s):  
B. Granadino ◽  
P. Santamaria ◽  
L. Sanchez
Keyword(s):  
Drosophila Melanogaster ◽  
Germ Cells ◽  
Germ Line ◽  
Wild Type ◽  
Female Development ◽  
Pole Cell ◽  
Somatic Tissues ◽  
Wild Type Female ◽  
Sex Lethal ◽  
Pole Cell Transplantation

The germ line exhibits sexual dimorphism as do the somatic tissues. Cells with the 2X;2A chromosome constitution will follow the oogenic pathway and X;2A cells will develop into sperm. In both somatic and germ-line tissues, the sexual pathway chosen by the cells depends on the gene Sex-lethal (Sxl), whose function is continuously needed for female development. In the soma, the sex of the cells is autonomously determined by the X:A signal while, in the germ line, the sex is determined by cell autonomous (the X:A signal) and somatic inductive signals. Three X-linked genes have been identified, scute (sc), sisterless-a (sis-a) and runt (run), that determine the initial functional state of Sxl in the soma. Using pole cell transplantation, we have tested whether these genes are also needed to activate Sxl in the germ line. We found that germ cells simultaneously heterozygous for sc, sis-a, run and a deficiency for Sxl transplanted into wild-type female hosts develop into functional oocytes. We conclude that the genes sc, sis-a and run needed to activate Sxl in the soma seem not to be required to activate this gene in the germ line; therefore, the X:A signal would be made up by different genes in somatic and germ-line tissues. The Sxlf7M1/Sxlfc females do not have developed ovaries. We have shown that germ cells of this genotype transplanted into wild-type female hosts produce functional oocytes. We conclude that the somatic component of the gonads in Sxlf7M1/Sxlfc females is affected, and consequently germ cells do not develop.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals An unusually simple HP1 gene set in Hymenopteran insects

2015 ◽  
Vol 93 (6) ◽  
pp. 596-603 ◽  
Author(s):  
C. Fang ◽  
L. Schmitz ◽  
P.M. Ferree
Keyword(s):  
Gene Family ◽  
Germ Line ◽  
Drosophila Species ◽  
Heterochromatin Protein 1 ◽  
Heterochromatin Protein ◽  
Insect Order ◽  
Representative Species ◽  
Somatic Tissues ◽  
Group A ◽  
Heterochromatin Structure

The heterochromatin protein 1 (HP1) gene family includes a set of paralogs in higher eukaryotes that serve fundamental roles in heterochromatin structure and maintenance, and other chromatin-related functions. At least 10 full and 16 partial HP1 genes exist among Drosophila species, with multiple gene gains, losses, and sub-functionalizations within this insect group. An important question is whether this diverse set of HP1 genes and their dynamic evolution represent the standard rule in eukaryotic groups. Here we have begun to address this question by bio-informatically identifying the HP1 family genes in representative species of the insect order Hymenoptera, which includes all ants, bees, wasps, and sawflies. Compared to Drosophila species, Hymenopterans have a much simpler set of HP1 genes, including one full and two partial HP1s. All 3 genes appear to have been present in the common ancestor of the Hymenopterans and they derive from a Drosophila HP1B-like gene. In ants, a partial HP1 gene containing only a chromoshadow domain harbors amino acid changes at highly conserved sites within the PxVxL recognition region, suggesting that this gene has undergone sub-functionalization. In the jewel wasp Nasonia vitripennis, the full HP1 and partial chromoshadow-only HP1 are expressed in both germ line and somatic tissues. However, the partial chromodomain-only HP1 is expressed exclusively in the ovary and testis, suggesting that it may have a specialized chromatin role during gametogenesis. Our findings demonstrate that the HP1 gene family is much simpler and evolutionarily less dynamic within the Hymenopterans compared to the much younger Drosophila group, a pattern that may reflect major differences in the range of chromatin-related functions present in these and perhaps other insect groups.

Chromosomal distribution of the major satellite DNA of South American rodents of the genus Ctenomys

1995 ◽  
Vol 69 (3-4) ◽  
pp. 179-184 ◽  
Author(s):  
M.S. Rossi ◽  
C.A. Redi ◽  
G. Viale ◽  
A.I. Massarini ◽  
E. Capanna
Keyword(s):  
Satellite Dna ◽  
South American ◽  
Chromosomal Distribution ◽  
Major Satellite

Changes in DNA content and chromosome number during spermatogenesis in the gall midge Monarthropalpus buxi (Cecidomyiidae, Diptera)

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 244-250 ◽  
Author(s):  
B. Jazdowska-Zagrodzinska ◽  
R. Dallai ◽  
C. A. Redi
Keyword(s):  
Chromosome Number ◽  
Key Words ◽  
Meiotic Division ◽  
Dna Content ◽  
Germ Line ◽  
Gall Midge ◽  
Key Words Dna ◽  
Further Development

In this paper we analyze the course of spermatogenesis in Monarthropalpus buxi. The first meiotic division occurs without any chromosomes pairing. As a result one spermatocyte II appears from which two sperms originate, and one residual cell, which does not undergo any further division. We found variations in chromosome number and DNA content between germ line cells of different individuals. Such variations were observed in the spermatocytes I and II, and in the sperms. In contrast, the residual cells, which did not take part in further development, always had the same DNA content and constantly inherited 20 chromosomes: 4 constituting one haploid set of the somatic type (S chromosomes) and 16 of the germ line limited type (E chromosomes).Key words: DNA content, chromosome number, Cecidomyiidae, germ line, spermatogenesis.

scholarly journals Differentially and Co-expressed Genes in Embryo, Germ-Line and Somatic Tissues of Tribolium castaneum

2019 ◽  
Vol 9 (7) ◽  
pp. 2363-2373 ◽  
Author(s):  
Sher Afzal Khan ◽  
Heather Eggleston ◽  
Kevin M. Myles ◽  
Zach N. Adelman
Keyword(s):  
Tribolium Castaneum ◽  
Germ Line ◽  
Somatic Tissues

Unusual Chromosomal Distribution of a Major Satellite DNA from Discoglossus pictus (Amphibia, Anura)

2009 ◽  
Vol 127 (1) ◽  
pp. 33-42 ◽  
Author(s):  
N. Amor ◽  
G. Odierna ◽  
G. Chinali ◽  
K. Said ◽  
O. Picariello
Keyword(s):  
Satellite Dna ◽  
Chromosomal Distribution ◽  
Major Satellite ◽  
Discoglossus Pictus

The Novel Proteasome Inhibitors Carfilzomib and Oprozomib Induce Milder Degenerative Effects Compared To Bortezomib When Administered Via Oral Feeding In An In Vivo Drosophila Experimental Model: A Biological Platform To Evaluate Safety/Efficacy Of Proteasome Inhibitors

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 1930-1930
Author(s):  
Evangelos Terpos ◽  
Eleni N. Tsakiri ◽  
Efstathios Kastritis ◽  
Tina Bagratuni ◽  
Vassilis G. Gorgoulis ◽  
...  
Keyword(s):  
Oral Administration ◽  
Proteasome Inhibitor ◽  
Germ Line ◽  
Conflicts Of Interest ◽  
Proteasome Inhibitors ◽  
Proteasome Inhibition ◽  
Somatic Tissue ◽  
Oral Feeding ◽  
Somatic Tissues

Abstract The proteasome is involved in the degradation of both normal, short-lived ubiquitinated proteins and mutated or damaged proteins. Carfilzomib is a tetrapeptide epoxyketone–based proteasome inhibitor and oprozomib is an orally bioavailable tripeptide epoxyketone-based proteasome inhibitor. The primary target for both agents is the chymotrypsin-like β5 subunit of the constitutive proteasome and immunoproteasome. Oprozomib is 5-fold less potent than carfilzomib, but displays similar cytotoxic potential with longer exposure times due to its time-dependent proteasome inhibition. In contrast, bortezomib is a slowly reversible proteasome inhibitor with potency of proteasome inhibition similar to carfilzomib. We propose the fruit fly Drosophila melanogaster as an in vivo platform for screening and characterizing proteasome inhibitors at the whole organism level. Drosophilais well-suited to this line of investigation, due to its powerful genetics, its similarities in key metabolic and aging pathways with humans, the fact that it expresses proteasomes that structurally resemble those from mammals, and also because it comprises a soma-germ line demarcation composed of both post-mitotic and mitotic cells. Moreover, flies live for few months and thus, drug screening on large cohorts can be completed in a reasonable time. We validate our model by investigating the effects of orally administered carfilzomib and oprozomib vs. bortezomib. In isolated Drosophila proteasome in vitroassays, carfilzomib showed a pattern of inhibitory activity similar to bortezomib, whereas oprozomib was less effective. After continuous oral administration of the inhibitors (∼50 μM of carfilzomib and ∼300-400 μM of oprozomib) to young flies (by adding the inhibitor in the flies’ culture medium) a proteasome inhibitory effect in somatic tissues roughly similar to 1 μM bortezomib was induced. Similar findings were noted when we analyzed distinct somatic tissue parts (i.e., head, thorax and abdomen), indicating that orally administered proteasome inhibitors are equally distributed to different body parts. As in the case of bortezomib, the effects of the inhibitors were less pronounced in the reproductive tissues. At the molecular level, carfilzomib (as compared to bortezomib) induced a milder disruption of fly somatic tissue proteostasis, lower rates of somatic tissue oxidative stress and less intense activation of genomic antioxidant response elements that correlated with reduced intensities of proteasome genes and protein subunit upregulation. Proteasome subunit induction was found to depend on the activity of the transcription factor Nrf2, a master regulator of cellular anti-oxidant responses. Furthermore, carfilzomib promoted the induction of lysosomal enzymes (e.g. cathepsins) and autophagy-related genes but less intensively compared to bortezomib. At concentrations that induced rates of proteasome inhibition that were similar to bortezomib, there were no significant toxic effects of either carfilzomib or oprozomib to oogenesis or to embryogenesis. Compared to bortezomib, both inhibitors exerted a significantly milder impact on the neuromusculatory system (locomotor performance) of the flies. Finally, we found that sustained oral administration of either carfilzomib or oprozomib exerted significantly milder effects (as compared to bortezomib) on flies’ mortality rate, healthspan and overall longevity. Our in vivo data support that carfilzomib is significantly less toxic compared to bortezomib, including neuromusculatory toxicity. Oprozomib was also less toxic but it is worth noting that it showed reduced activity against fly proteasomes. In support, our preliminary analyses indicated that in comparison to bortezomib and carfilzomib, oprozomib was less potent when tested in human osteosarcoma cancer cell lines. The validity of our in vivo pharmacological model is exemplified by the observed similarities with the reported clinical adverse effects, while the ratio of the different doses used to achieve similar rates of proteasome inhibition in Drosophila somatic tissues (i.e. ∼1 μM bortezomib, ∼50 μM carfilzomib) is reminiscent of the doses used in the clinic (i.e. ∼1.3 mg/m2 bortezomib and ∼25-56 mg/m2 carfilzomib). We conclude that fruit flies represent a valid biological platform for evaluating the efficacy and toxicity of proteasome inhibitors. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Biogeographic patterns in the chromosomal distribution of a satellite DNA in the banded tetra Astyanax fasciatus (Teleostei: Characiformes)

2012 ◽  
Vol 13 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Karine Frehner Kavalco ◽  
Rubens Pazza ◽  
Karina de Oliveira Brandão ◽  
Lurdes Foresti de Almeida-Toledo
Keyword(s):  
Satellite Dna ◽  
Chromosomal Distribution ◽  
Biogeographic Patterns ◽  
Astyanax Fasciatus
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