Histogenetic Study of the Vestibular Ganglion in the Developing Chick Embryo by Lectin-Horseradish Peroxidase Methods

ORL ◽  
1989 ◽  
Vol 51 (6) ◽  
pp. 325-331 ◽  
Author(s):  
Hiroshi Yamashita ◽  
Toru Sekitani
Development ◽  
1984 ◽  
Vol 83 (1) ◽  
pp. 213-223
Author(s):  
N. G. Laing

Chick embryo wing buds were rotated close to the lateral edge of the somites at stage 19, prior to limb innervation. Despite the abnormal orientation of the resulting limb, the motor pools to biceps and triceps were largely normal, as judged by electrical stimulation and horseradish peroxidase labelling just prior to hatching. The only abnormalities were a few caudal motoneurons innervating biceps and a few rostral motoneurons innervating triceps. This distribution is similar to that seen normally in young embryos before the completion of motoneuron death and it is suggested that the rotation may be keeping alive motoneurons which otherwise would die. The morphology of the brachial plexus supplying rotated wings was abnormal. It is concluded that axons growing into the limb bud from the spinal cord can compensate for reversal of both the limb axes and selectively innervate appropriate muscles. The result is consistent with others in which proximal reversal of one limb axis alone produced normal innervation.


1992 ◽  
Vol 92 (2) ◽  
pp. 127-137 ◽  
Author(s):  
Silvia Gheri Bryk ◽  
Gherardo Gheri ◽  
Eleonora Sgambati ◽  
Giovanni E. Orlandini

1973 ◽  
Vol 19 (1) ◽  
pp. 147-149 ◽  
Author(s):  
R. Dobardzic ◽  
A. Boudreault ◽  
V. Pavilanis

The specific antibodies anti-influenza virus A2/Aichi/68 were combined immunologically with the virus purified by zonal centrifugation, then dissociated by lowering the pH and conjugated with horseradish peroxidase using glutaraldehyde as the coupling agent. With so labeled antibodies it was possible to detect the viral antigen in infected chick embryo kidney cells with the light microscope. This work suggests that the immunoperoxidase technique could be useful for early rhinocytologic diagnosis of influenza virus infections.


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