Ultrastructural Study of Axonal Cytoskeletons in the Optic Nerve Damaged by Acutely Elevated Intraocular Pressure Using the Quick-Freezing and Deep-Etching Technique

1997 ◽  
Vol 29 (1) ◽  
pp. 48-54 ◽  
Author(s):  
Bo Ou ◽  
Shinichi Ohno ◽  
Nobuo Terada ◽  
Yasuhisa Fujii ◽  
Hideho Ueda ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Optic Nerve ◽  
Ultrastructural Study ◽  
Etching Technique ◽  
Deep Etching ◽  
Quick Freezing ◽  
Elevated Intraocular Pressure

Ultrastructural Study of the Cytoskeleton of Optic Nerve Axons in Guinea Pigs as Revealed by a Quick-Freezing, Deep-Etching Method

1996 ◽  
Vol 28 (1) ◽  
pp. 29-35
Author(s):  
Bo Ou ◽  
Shinichi Ohno ◽  
Nobuo Terada ◽  
Yasuhisa Fujii ◽  
Hai-Bo Chen ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Deep Etching ◽  
Etching Method ◽  
Quick Freezing

Ultrastructural study of cytoskeletal interactions with endosomes in macrophages by quickfreezing and deep-etching method

1990 ◽  
Vol 48 (3) ◽  
pp. 576-577
Author(s):  
Takeshi Baba ◽  
Nobuki Shiozawa ◽  
Masao Hotch ◽  
Shinichi Ohno
Keyword(s):  
Immune Complex ◽  
Ultrastructural Study ◽  
Fc Receptor ◽  
Coated Pits ◽  
Deep Etching ◽  
Receptor Mediated Endocytosis ◽  
Etching Method ◽  
Quick Freezing

Endosomes are vesicular or tubular organelles that play important roles in transports of receptors and receptor―bound ligands during receptor-mediated endocytosis. The mechanisms of endocytic transports from clathrin-coated pits to lysosomes have been studied by many investigators. However, few studies were reported about the interactions between endosomes and cytoskeletons. In this study, Fc-receptor-mediated endocytosis in macrophages are investigated by quick-freezing and deep-etching (QF-DE) method combined with gold-labeled immune complex and “replica scraping method”.

scholarly journals Optic Nerve Head Astrocytes Display Axon-Dependent and -Independent Reactivity in Response to Acutely Elevated Intraocular Pressure

2019 ◽  
Vol 60 (1) ◽  
pp. 312 ◽  
Author(s):  
Shandiz Tehrani ◽  
Lauren Davis ◽  
William O. Cepurna ◽  
R. Katherine Delf ◽  
Diana C. Lozano ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Optic Nerve ◽  
Optic Nerve Head ◽  
Elevated Intraocular Pressure

Measuring the Biaxial Stress-Strain Characteristics of Human Sclera

2007 ◽  
Author(s):  
C. G. Olesen ◽  
I. Tertinegg ◽  
A. Eilaghi ◽  
G. W. Brodland ◽  
C. Horst ◽  
...  
Keyword(s):  
Risk Factor ◽  
Intraocular Pressure ◽  
Optic Nerve ◽  
Ganglion Cell ◽  
Mechanical Strain ◽  
Biaxial Stress ◽  
Retinal Ganglion ◽  
Irreversible Loss ◽  
Elevated Intraocular Pressure ◽  
Primary Risk Factor

Glaucoma is a common ocular disease that causes irreversible loss of vision. Elevated intraocular pressure (IOP) is the primary risk factor for developing glaucoma. It is believed that increased IOP causes mechanical strain on the glial cells that support the retinal ganglion cell axons and thereby causes ganglion cell apoptosis [1,2]. This damage occurs in the optic nerve head (ONH) region of the eye, and is important for understanding ONH biomechanics.

Ultrastructural study ofCryptococcus neoformans by quick-freezing and deep-etching method

1993 ◽  
Vol 121 (3) ◽  
pp. 133-141 ◽  
Author(s):  
Nobuki Sakaguchi ◽  
Takeshi Baba ◽  
Masao Fukuzawa ◽  
Shinichi Ohno
Keyword(s):  
Ultrastructural Study ◽  
Deep Etching ◽  
Etching Method ◽  
Quick Freezing

Ultrastructural Study of Peroxisome Proliferation in Hepatocytes by Quick-Freezing and Deep-Etching Method

1988 ◽  
Vol 46 ◽  
pp. 258-259
Author(s):  
S. Ohno
Keyword(s):  
Electron Microscopy ◽  
Liquid Nitrogen ◽  
Ultrastructural Study ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferation ◽  
Solution Ph ◽  
Deep Etching ◽  
Quick Freezing ◽  
Ethylhexyl Phthalate ◽  
Conventional Electron Microscopy

It had been emphasized that luminar continuities between sER and peroxisomes were detected by conventional electron microscopy. However, recent studies ruled out the luminar continuities between sER and peroxisomes. Lazarow et al. reported that peroxisomal proteins were synthesized on free ribosomes and postulated the existence of “peroxisomal reticulum” distinct from the ER. The object of this study is to clarify the proliteration mechanism of peroxisomes after administration of a peroxisome proliferator, DEHP (di-2- ethylhexyl phthalate).Mice treated with 2% DEHP for 1, 3, 5 and 7 days and normal mice were perfused with 2% paraformaldehyde in 0.1M phosphate buffered solution, pH 7.4, (PB) for 5 min. The livers were cut into small pieces, washed in PB to remove cytoplasmic soluble proteins and were fixed again with 2% paraformaldehyde-0.25% glutaraldehyde for 30 min. They were quickly frozen in isopentane-propane mixture (around -190 C) and fractured in liquid nitrogen to remove the damaged surface tissues. They were deeply etched in Eiko FD-3S machine (-95°C, 2-6xl0-7 Torr) and rotary shadowed with platinum.

Elevated Intraocular Pressure, Optic Nerve Atrophy, and Impaired Retinal Development in ODAG Transgenic Mice

2009 ◽  
Vol 50 (1) ◽  
pp. 242 ◽  
Author(s):  
Takaaki Sasaki ◽  
Wataru Watanabe ◽  
Yuki Muranishi ◽  
Takashi Kanamoto ◽  
Makoto Aihara ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Optic Nerve ◽  
Transgenic Mice ◽  
Retinal Development ◽  
Optic Nerve Atrophy ◽  
Elevated Intraocular Pressure
Sign in / Sign up

Export Citation Format

Share Document