Effect of Dental Plaque on Fluoride Uptake by Enamel from a Sodium Fluoride Varnish in vivo

1983 ◽  
Vol 17 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Liisa Seppä
Keyword(s):  
Sodium Fluoride ◽  
Dental Plaque ◽  
Fluoride Varnish ◽  
Fluoride Uptake

scholarly journals Treatment of dental plaque biofilms using photodynamic therapy: a randomised controlled study

2021 ◽  
Author(s):  
A. Alsaif ◽  
J. F. Tahmassebi ◽  
S. R. Wood
Keyword(s):  
Photodynamic Therapy ◽  
Incubation Time ◽  
Dental Plaque ◽  
Continuous Light ◽  
Laser Scanning Microscopy ◽  
Controlled Study ◽  
Bacterial Counts ◽  
Randomised Controlled Study ◽  
Randomised Controlled

Abstract Introduction Photodynamic therapy (PDT) is a treatment modality involving a dye that is activated by exposure to light of a specific wavelength in the presence of oxygen to form oxygen species causing localised damage to microorganisms. Aim To determine the most effective bactericidal incubation and irradiation times of erythrosine-based PDT on in vivo-formed dental plaque biofilms. Methods A randomised controlled study; 18-healthy adult participants wearing intraoral appliances with human enamel slabs to collect dental plaque samples in two separate periods of two weeks each for use in arm-1 and arm-2. These accumulated dental plaque samples were treated with PDT under different experimental conditions. Incubation times with photosensitiser (erythrosine) of 15 min and 2 min were used in arm-1 and arm-2, respectively, followed by light irradiation for either 15 min (continuous) or as a fractionated dose (5 × 30 sec). Following treatment, percentage reductions of total bacterial counts were compared between the different groups. In addition, confocal laser scanning microscopy (CLSM) and LIVE/DEAD® BacLight™ Bacterial Viability Kit were used to visualise the effect of PDT on in vivo-formed biofilms. Results Significant reductions in the percentage of total bacterial counts (~93–95%) of in vivo-formed biofilms were found when using either 2 min or 15min incubation times and applying 15 min continuous light. Although when applying fractionated light, there was more cell death when 15 min incubation time was used (~ 91%) compared with the 2 min incubation time (~ 64%). CLSM results supported these findings. Conclusion Improving the clinical usefulness of PDT by reducing its overall treatment time seems to be promising and effective in killing in vivo-formed dental plaque biofilms.

Effect of Some Polyvalent Cations on the Acidogenicity of Dental Plaque in vivo

1980 ◽  
Vol 14 (6) ◽  
pp. 422-427 ◽  
Author(s):  
Rui V. Oppermann ◽  
Gunnar Rölla
Keyword(s):  
Dental Plaque ◽  
Polyvalent Cations

A Method for the Quantitative Site-Specific Study of the Biochemistry within Dental Plaque Biofilms Formed in vivo

1997 ◽  
Vol 31 (3) ◽  
pp. 194-200 ◽  
Author(s):  
C. Robinson ◽  
J. Kirkham ◽  
R. Percival ◽  
R.C. Shore ◽  
W.A. Bonass ◽  
...  
Keyword(s):  
Dental Plaque ◽  
Site Specific ◽  
Specific Study

scholarly journals Bacteriocin-like activity of oral Fusobacterium nucleatum isolated from human and non-human primates

1999 ◽  
Vol 30 (4) ◽  
pp. 324-346 ◽  
Author(s):  
Elerson Gaetti-Jardim Júnior ◽  
Mario Julio Avila-Campos
Keyword(s):  
Oral Cavity ◽  
Microbial Ecology ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Antagonistic Activity ◽  
Healthy Individuals ◽  
Bacterial Microbiota ◽  
Reference Strains

Fusobacterium nucleatum is indigenous of the human oral cavity and has been involved in different infectious processes. The production of bacteriocin-like substances may be important in regulation of bacterial microbiota in oral cavity. The ability to produce bacteriocin-like substances by 80 oral F. nucleatum isolates obtained from periodontal patients, healthy individuals and Cebus apella monkeys, was examinated. 17.5% of all tested isolates showed auto-antagonism and 78.8% iso- or hetero-antagonism. No isolate from monkey was capable to produce auto-inhibition. In this study, the antagonistic substances production was variable in all tested isolates. Most of the F. nucleatum showed antagonistic activity against tested reference strains. These data suggest a possible participation of these substances on the oral microbial ecology in humans and animals. However, the role of bacteriocins in regulating dental plaque microbiota in vivo is discussed.

scholarly journals HISTOCHEMICAL INVESTIGATIONS ON THE IN VIVO EFFECTS OF FLUORIDE ON TRICARBOXYLIC ACID CYCLE DEHYDROGENASES FROM PELARGONIUM ZONALE: PART II

1967 ◽  
Vol 15 (4) ◽  
pp. 202-206
Author(s):  
C. JAMES LOVELACE ◽  
GENE W. MILLER
Keyword(s):  
Sodium Fluoride ◽  
Reductase Activity ◽  
Tricarboxylic Acid Cycle ◽  
Leaf Tissue ◽  
Tca Cycle ◽  
Tricarboxylic Acid ◽  
Pelargonium Zonale ◽  
Acid Cycle ◽  
In Vivo Effects

In vivo effects of fluoride on tricarboxylic acid (TCA) cycle dehydrogenase enzymes of Pelargonium zonale were studied using p-nitro blue tetrazoleum chloride. Plants were exposed to 17 ppb HF, and enzyme activities in treated plants were compared to those in controls. Leaves of control plants were incubated in 5 x 10–3 M sodium fluoride. Injuries observed in fumigation and solution experiments were similar. Leaf tissue subjected to HF or sodium fluoride evidenced less succinic p-nitro blue tetrazoleum reductase activity than did control tissue. Other TCA cycle dehydrogenase enzymes were not observably affected by the fluoride concentrations used in these experiments. Excised leaves cultured in 5 x 10–3 M sodium fluoride exhibited less succinic p-nitro blue tetrazoleum reductase activity after 24 hr than did leaves cultured in 5 x 10–3 M sodium chloride.

scholarly journals Effectiveness of Silver Diamine Fluoride and Sodium Fluoride Varnish in Arresting Dentin Caries in Chinese Pre-school Children

2002 ◽  
Vol 81 (11) ◽  
pp. 767-770 ◽  
Author(s):  
C.H. Chu ◽  
E.C.M. Lo ◽  
H.C. Lin
Keyword(s):  
Sodium Fluoride ◽  
School Children ◽  
Fluoride Varnish ◽  
Controlled Clinical Trial ◽  
Silver Diamine Fluoride ◽  
Fluoride Solution ◽  
Dentin Caries ◽  
Anterior Teeth ◽  
Tooth Surfaces ◽  
Topical Fluoride

Untreated dental caries in Chinese pre-school children is common. This prospective controlled clinical trial investigated the effectiveness of topical fluoride applications in arresting dentin caries. Three hundred seventy-five children, aged 3-5 years, with carious upper anterior teeth were divided into five groups. Children in the first and second groups received annual applications of silver diamine fluoride solution (44,800 ppm F). Sodium fluoride varnish (22,600 ppm F) was applied every three months to the lesions of children in the third and fourth groups. For children in the first and third groups, soft carious tissues were removed prior to fluoride application. The fifth group was the control. Three hundred eight children were followed for 30 months. The respective mean numbers of arrested carious tooth surfaces in the five groups were 2.5, 2.8, 1.5, 1.5, and 1.3 (p < 0.001). Silver diamine fluoride was found to be effective in arresting dentin caries in primary anterior teeth in pre-school children.

Fluoride uptake in human dentine from glass-ionomer cement in vivo

1993 ◽  
Vol 38 (12) ◽  
pp. 1093-1098 ◽  
Author(s):  
M. Murai ◽  
M. Ikeda ◽  
T. Yanagihara ◽  
G. Hara ◽  
K. Kato ◽  
...  
Keyword(s):  
Glass Ionomer Cement ◽  
Glass Ionomer ◽  
Fluoride Uptake ◽  
Human Dentine ◽  
Ionomer Cement
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