Effect of Some Polyvalent Cations on the Acidogenicity of Dental Plaque in vivo

1980 ◽  
Vol 14 (6) ◽  
pp. 422-427 ◽  
Author(s):  
Rui V. Oppermann ◽  
Gunnar Rölla
Keyword(s):  
Dental Plaque ◽  
Polyvalent Cations

scholarly journals Treatment of dental plaque biofilms using photodynamic therapy: a randomised controlled study

2021 ◽  
Author(s):  
A. Alsaif ◽  
J. F. Tahmassebi ◽  
S. R. Wood
Keyword(s):  
Photodynamic Therapy ◽  
Incubation Time ◽  
Dental Plaque ◽  
Continuous Light ◽  
Laser Scanning Microscopy ◽  
Controlled Study ◽  
Bacterial Counts ◽  
Randomised Controlled Study ◽  
Randomised Controlled

Abstract Introduction Photodynamic therapy (PDT) is a treatment modality involving a dye that is activated by exposure to light of a specific wavelength in the presence of oxygen to form oxygen species causing localised damage to microorganisms. Aim To determine the most effective bactericidal incubation and irradiation times of erythrosine-based PDT on in vivo-formed dental plaque biofilms. Methods A randomised controlled study; 18-healthy adult participants wearing intraoral appliances with human enamel slabs to collect dental plaque samples in two separate periods of two weeks each for use in arm-1 and arm-2. These accumulated dental plaque samples were treated with PDT under different experimental conditions. Incubation times with photosensitiser (erythrosine) of 15 min and 2 min were used in arm-1 and arm-2, respectively, followed by light irradiation for either 15 min (continuous) or as a fractionated dose (5 × 30 sec). Following treatment, percentage reductions of total bacterial counts were compared between the different groups. In addition, confocal laser scanning microscopy (CLSM) and LIVE/DEAD® BacLight™ Bacterial Viability Kit were used to visualise the effect of PDT on in vivo-formed biofilms. Results Significant reductions in the percentage of total bacterial counts (~93–95%) of in vivo-formed biofilms were found when using either 2 min or 15min incubation times and applying 15 min continuous light. Although when applying fractionated light, there was more cell death when 15 min incubation time was used (~ 91%) compared with the 2 min incubation time (~ 64%). CLSM results supported these findings. Conclusion Improving the clinical usefulness of PDT by reducing its overall treatment time seems to be promising and effective in killing in vivo-formed dental plaque biofilms.

A Method for the Quantitative Site-Specific Study of the Biochemistry within Dental Plaque Biofilms Formed in vivo

1997 ◽  
Vol 31 (3) ◽  
pp. 194-200 ◽  
Author(s):  
C. Robinson ◽  
J. Kirkham ◽  
R. Percival ◽  
R.C. Shore ◽  
W.A. Bonass ◽  
...  
Keyword(s):  
Dental Plaque ◽  
Site Specific ◽  
Specific Study

scholarly journals Bacteriocin-like activity of oral Fusobacterium nucleatum isolated from human and non-human primates

1999 ◽  
Vol 30 (4) ◽  
pp. 324-346 ◽  
Author(s):  
Elerson Gaetti-Jardim Júnior ◽  
Mario Julio Avila-Campos
Keyword(s):  
Oral Cavity ◽  
Microbial Ecology ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Antagonistic Activity ◽  
Healthy Individuals ◽  
Bacterial Microbiota ◽  
Reference Strains

Fusobacterium nucleatum is indigenous of the human oral cavity and has been involved in different infectious processes. The production of bacteriocin-like substances may be important in regulation of bacterial microbiota in oral cavity. The ability to produce bacteriocin-like substances by 80 oral F. nucleatum isolates obtained from periodontal patients, healthy individuals and Cebus apella monkeys, was examinated. 17.5% of all tested isolates showed auto-antagonism and 78.8% iso- or hetero-antagonism. No isolate from monkey was capable to produce auto-inhibition. In this study, the antagonistic substances production was variable in all tested isolates. Most of the F. nucleatum showed antagonistic activity against tested reference strains. These data suggest a possible participation of these substances on the oral microbial ecology in humans and animals. However, the role of bacteriocins in regulating dental plaque microbiota in vivo is discussed.

Antibacterial Activity and Anti-Biofilm Effect of Chitosan against Strains of Streptococcus Mutans Isolated in Dental Plaque

2008 ◽  
Vol 21 (4) ◽  
pp. 993-997 ◽  
Author(s):  
G. Pasquantonio ◽  
C. Greco ◽  
M. Prenna ◽  
C. Ripa ◽  
L.A. Vitali ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Chitosan Solution ◽  
Positive Control ◽  
Phases Of Development ◽  
Adhesion Phase ◽  
Adult Volunteers

Streptococcus mutans is the major cause of dental plaque and is often associated with biofilm formation. The aim of this study is to evaluate the activity of a hydrosoluble derivative of chitosan against S. mutans biofilms in vitro and in vivo. Strains of S. mutans were isolated from the dental plaque of 84 patients enrolled in the study. The antibacterial activity of chitosan was determined by broth microdilutions. The effect of chitosan at different concentrations and exposure times on S. mutans biofilms at different phases of development was assessed by a clinical study using the classical “4-day plaque regrowth” experiment in adult volunteers. The MIC values of chitosan were between 0.5 and 2 g/L. Compared to distilled water, the chitosan solution significantly decreased the vitality of plaque microflora (p≤0.05). Chlorhexidine, used as a positive control, reduced vitality even further. The results showed that S. mutans in the adhesion phase (4 h) was completely inhibited by chitosan at any concentration (0.1, 0.2, 0.5XMIC) or exposure time investigated (1, 15, 30, 60 min), while S. mutans at successive stages of accumulation (12–24 h) was inhibited only by higher concentrations and longer exposure times. These data confirm the effective action of chitosan against S. mutans biofilms.

scholarly journals Aggregative Behavior of Bacteria Isolated from Canine Dental Plaque

2006 ◽  
Vol 72 (8) ◽  
pp. 5211-5217 ◽  
Author(s):  
David R. Elliott ◽  
Michael Wilson ◽  
Catherine M. F. Buckley ◽  
David A. Spratt
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Dental Plaque ◽  
Oral Bacteria ◽  
Surface Structures ◽  
Aggregative Behavior ◽  
Transmission Electron

ABSTRACT Interbacterial adhesion of bacteria isolated from canine dental plaque was assessed by performing a visual coaggregation assay. Using conditions mimicking those likely to be encountered in vivo, the entire cultivable plaque microbiota from a single dog was assessed, and eight (6.7%) unique coaggregation interactions were detected for 120 crosses. Transmission electron microscopy was used to visualize several of the bacteria in isolation and as coaggregates, which revealed surface structures that may be involved in adhesion and coaggregation. The results of this study indicate that the prevalence of coaggregating pairs of dental plaque bacteria in dogs is similar to the prevalence of coaggregating pairs of dental plaque bacteria reported in humans. In addition, genera found in both hosts generally exhibited similar coaggregation reactions; however, autoaggregation was found to be more common among oral bacteria isolated from dogs.

The effect of different methods of drinking a carbonated beverage on the Ph of dental plaque: An in vivo study

2006 ◽  
Vol os13 (2) ◽  
pp. 75-75
Author(s):  
YB Aswini ◽  
PS Tangade ◽  
AV Ankola ◽  
L Nagesh ◽  
H Pradnya
Keyword(s):  
Dental Plaque ◽  
In Vivo Study ◽  
Carbonated Beverage

In vivo Effects of Zinc and Chlorhexidine on Dental Plaque Ureolysis and Glycolysis

1989 ◽  
Vol 68 (6) ◽  
pp. 1132-1134 ◽  
Author(s):  
E. Giertsen ◽  
A.Aa. Scheie ◽  
G. Rolla
Keyword(s):  
Dental Plaque ◽  
In Vivo Effects
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