Contribution of Radiation Hybrids to Genome Mapping in Domestic Animals

2009 ◽  
Vol 126 (1-2) ◽  
pp. 21-33 ◽  
Author(s):  
T. Faraut ◽  
S. de Givry ◽  
C. Hitte ◽  
Y. Lahbib-Mansais ◽  
M. Morisson ◽  
...  
Keyword(s):  
Genome Mapping ◽  
Domestic Animals ◽  
Radiation Hybrids

Transmission of maize chromosome 9 rearrangements in oat–maize radiation hybrids

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1202-1210 ◽  
Author(s):  
M Isabel Vales ◽  
Oscar Riera-Lizarazu ◽  
Howard W Rines ◽  
Ronald L Phillips
Keyword(s):  
Zea Mays ◽  
Avena Sativa ◽  
Genome Mapping ◽  
Chromosome 9 ◽  
Chromosome Rearrangements ◽  
Maize Chromosome ◽  
Intergenomic Translocations ◽  
Radiation Hybrids ◽  
Self Fertilization ◽  
Radiation Induced

Oat–maize radiation hybrids are oat (Avena sativa L.) plants carrying radiation-induced subchromosome fragments of a given maize (Zea mays L.) chromosome. Since first-generation radiation hybrids contain various maize chromosome rearrangements in a hemizygous condition, variation might be expected in the transmission of these rearrangements to subsequent generations. The transmission and integrity of maize chromosome 9 rearrangements were evaluated in progenies of 30 oat–maize radiation hybrids by using a series of DNA-based markers and by genomic in situ hybridization. Maize chromosome 9 rearrangements were reisolated by self-fertilization in 24 of the 30 radiation hybrid lineages. Normal and deleted versions of maize chromosome 9 were transmitted at similar frequencies of 9.1% and 7.6%, respectively, while intergenomic translocations were transmitted at a significantly higher frequency of 47.6%. Most lines (93%) that inherited a rearrangement had it in the hemizygous condition. Lines with a rearrangement in the homozygous state (7%) were only identified in lineages with intergenomic translocations. Homozygous lines are more desirable from the perspective of stock maintenance, since they may stably transmit a given rearrangement to a subsequent generation. However, their isolation is not strictly required, since hemizygous lines can also be used for genome mapping studies.Key words: Avena sativa, Zea mays, addition lines, chromosome rearrangements.

Genome mapping in domestic animals

1994 ◽  
Vol 150 (3) ◽  
pp. 215-217
Author(s):  
P.E. Fischer
Keyword(s):  
Genome Mapping ◽  
Domestic Animals

High-resolution nucleic acid sequence mapping via in situ hybridization at the Electron Microscope level

1995 ◽  
Vol 53 ◽  
pp. 752-753
Author(s):  
B.A. Hamkalo ◽  
S. Narayanswami ◽  
A.P. Kausch
Keyword(s):  
Nucleic Acid ◽  
Interphase Nucleus ◽  
Genome Mapping ◽  
Precise Location ◽  
Electron Microscope Level ◽  
Rna Sequences ◽  
Fundamental Interest ◽  
Whole Cells ◽  
Dna And Rna

The availability of nonradioactive methods to label nucleic acids an the resultant rapid and greater sensitivity of detection has catapulted the technique of in situ hybridization to become the method of choice to locate of specific DNA and RNA sequences on chromosomes and in whole cells in cytological preparations in many areas of biology. It is being applied to problems of fundamental interest to basic cell and molecular biologists such as the organization of the interphase nucleus in the context of putative functional domains; it is making major contributions to genome mapping efforts; and it is being applied to the analysis of clinical specimens. Although fluorescence detection of nucleic acid hybrids is routinely used, certain questions require greater resolution. For example, very closely linked sequences may not be separable using fluorescence; the precise location of sequences with respect to chromosome structures may be below the resolution of light microscopy(LM); and the relative positions of sequences on very small chromosomes may not be feasible.

PROBLEMS OF STRAY ANIMALS IN NOVOSIBIRSK

2020 ◽  
Vol 4 (2) ◽  
pp. 85-91
Author(s):  
Elena E. Rinchinova ◽  
Diyara A. Takumova ◽  
Irina I. Bochkareva
Keyword(s):  
Urban Population ◽  
Domestic Animals ◽  
Legal Documents ◽  
Quantitative Indicators ◽  
The City

The article discusses main issues of organizing activities for the treatment of stray and street animals in the city of Novosibirsk. The important role of successful solving the problem of stray animals in ensuring environmental comfort and safety of the urban population is noted. Definitions of the concepts “stray animals” and “street animals” are given, the differences between them are emphasized. The main regulatory and legal documents governing the handling of stray and street animals are listed. The ways in which domestic animals get into a stray state are described briefly. The results of the collection and analysis of information on the activities of shelters for stray animals in Novosibirsk are described. The information on the quantitative indicators of the shelters are given. Conclusions on how to solve the problem of stray animals, relying on the latest regulations are drawn.

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