Effect of Digoxin on DNA Synthesis and Cell Viability in Human Breast Tumour Tissue in Organ Culture

Chemotherapy ◽  
1983 ◽  
Vol 29 (5) ◽  
pp. 368-372 ◽  
Author(s):  
I.R. Falconer ◽  
A.M. Beresford ◽  
A. Jones ◽  
A.T. Vacek
Keyword(s):  
Organ Culture ◽  
Dna Synthesis ◽  
Cell Viability ◽  
Breast Tumour ◽  
Tumour Tissue ◽  
Human Breast ◽  
Human Breast Tumour ◽  
Breast Tumour Tissue

scholarly journals The differential processing of proenkephalin A in mouse and human breast tumour cell lines

1999 ◽  
Vol 161 (3) ◽  
pp. 475-484 ◽  
Author(s):  
BK Brar ◽  
PJ Lowry
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Tumour Cell ◽  
Breast Tumour ◽  
Human Breast ◽  
Human Breast Tumour ◽  
Breast Tumour Cell ◽  
Cell Lysates ◽  
Human Breast Tumour Cell ◽  
Tumour Cell Lines

We have carried out an investigation into the processing of the enkephalin-like immunoreactivity reported in breast tissue using two human breast tumour cell lines and a mouse tumour cell line. A 46 kDa form of proenkephalin (PE) has been observed in the cell lysates of two human breast tumour cell lines (MCF-7, ZR-75-1) and the mouse androgen-responsive Shionogi breast carcinoma cell line (SC115). PE processing in the cell lysates of these cells was assessed by a specific met-enkephalin RIA. The basal levels of processed PE in the MCF-7, ZR-75-1 and SC115 cell lysates were 30, 30 and 76% respectively. The processing enzymes PC1 and PC2, which have been implicated in the differential processing of PE, were detected by immunoblot analysis in these cells. PC1 was found within the cell extracts of all three cell lines. PC2 was only observed in the SC115 cell line, which may account for the higher percentage of processed PE measured. The cDNA of PC2 has been transfected into ZR-75-1 cells and this was accompanied by an increase in the level of processed PE from 30 to 76%. These breast tumour cell lines may provide a useful insight into the function of enkephalin-containing peptides in breast cancer.

RELATIVE OESTROGEN CONCENTRATIONS IN BREAST TUMOUR TISSUE FOLLOWING ANDROGEN THERAPY

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S235
Author(s):  
Hannelore Braunsberg ◽  
A. E. Carter ◽  
W. T. Irvine ◽  
V. H. T. James
Keyword(s):  
Breast Tumour ◽  
Tumour Tissue ◽  
Androgen Therapy ◽  
Breast Tumour Tissue
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