The Response to Hormones and Anti-Hormones of Human Breast Tumour Cells Grown in Continuous Culture

1978 ◽  
Vol 6 (6) ◽  
pp. 1318-1319
Author(s):  
MARTIN J. PAGE ◽  
JOHN K. FIELD ◽  
CHRIS D. GREEN
Keyword(s):  
Continuous Culture ◽  
Breast Tumour ◽  
Tumour Cells ◽  
Human Breast ◽  
Human Breast Tumour ◽  
Breast Tumour Cells

scholarly journals ANTI-TUMOUR ACTIVITY OF BROMOPYRROLE ALKALOIDS AGAINST HUMAN BREAST TUMOUR (MCF-7) THROUGH APOPTOSIS INDUCTION

2019 ◽  
pp. 94-100
Author(s):  
S. MANIKANDAN ◽  
S. GANESAPANDIAN ◽  
MANOJ SINGH ◽  
A. K. KUMARAGURU
Keyword(s):  
Cell Cycle ◽  
Breast Tumour ◽  
Tumour Cells ◽  
Gas Chromatography Mass Spectrometry ◽  
Gulf Of Mannar ◽  
Human Breast ◽  
Caspase 9 ◽  
Human Breast Tumour ◽  
Breast Tumour Cells ◽  
Mcf 7

Objective: The present study was conducted to screen the cytotoxicity and initiation of apoptosis in MCF-7 human breast tumour cells using the Indian sponge Acanthostylotela cornuta of the Gulf of Mannar. Methods: The crude methanol extract of A. cornuta was fractionated using a reversed phase silica gel column chromatography. The purity of the active fraction of bromopyrrole alkaloids was confirmed with Gas Chromatography-Mass Spectrometry (GCMS). The bromopyrrole alkaloids induce apoptotic changes in MCF-7 cells were studied with electrophoresis, caspase assay, and different staining analysis. The MCF-7 cells were analyzed by Flow cytometry to determine their DNA content. Results: Bromopyrrole alkaloids of A. cornuta showed cytotoxicity against MCF-7 human breast tumour cells with the IC50 value of 8.0μg/ml. The bromopyrrole compounds induced cells exhibited scatted red fluorescence, showing the presence of several residual bodies and condensation of chromatin. Caspase-3, pro-caspase-9, caspase-9 and Poly-ADP-Ribose-Polymerase (PARP) activity were occurred in bromopyrrole alkaloids treated tumour cell. The cell cycle arrest is conceivable that the compound prevented the progression of cell cycle through the G phase resulting from inhibition of survival, leading them to undergo apoptosis. Conclusion: Bromopyrrole alkaloids of A. cornuta possess antitumor activity which was arrested the G phase in cell cycle that clearly indicated its nature as that of antitumour drugs.

IN VITRO STUDIES OF Tc-99m TETROFOSMIN IN SENSITIVE AND MULTIDRUG-RESISTANT RODENT AND HUMAN BREAST TUMOUR CELLS

1995 ◽  
Vol 20 (7) ◽  
pp. 661
Author(s):  
J. Bannerman ◽  
J. R. Bollinger ◽  
I. Boxen ◽  
B. W. Berry ◽  
P. Firby ◽  
...  
Keyword(s):  
Breast Tumour ◽  
Multidrug Resistant ◽  
Tumour Cells ◽  
In Vitro Studies ◽  
Human Breast ◽  
Human Breast Tumour ◽  
Breast Tumour Cells

scholarly journals Effects of oncogenic ErbB2 on G1 cell cycle regulators in breast tumour cells

Oncogene ◽  
2000 ◽  
Vol 19 (13) ◽  
pp. 1647-1656 ◽  
Author(s):  
Richard M Neve ◽  
Hedwig Sutterlüty ◽  
Nicholas Pullen ◽  
Heidi A Lane ◽  
John M Daly ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Breast Tumour ◽  
Tumour Cells ◽  
Cell Cycle Regulators ◽  
Breast Tumour Cells

Doxorubicin synergises with a TRAIL-R1-specific ligand to selectively target TRAIL-resistant breast tumour cells for apoptosis

Toxicology ◽  
2011 ◽  
Vol 290 (2-3) ◽  
pp. 141-142
Author(s):  
Shambhavi Naik ◽  
Davina Twiddy ◽  
Roshna Mistry ◽  
Jennifer M. Edwards ◽  
Gerald M. Cohen ◽  
...  
Keyword(s):  
Breast Tumour ◽  
Tumour Cells ◽  
Specific Ligand ◽  
Breast Tumour Cells
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