Chronic Granulomatous Disease Assessed by Single-Cell Granulocyte Oxidative Burst Activity

1995 ◽  
Vol 106 (4) ◽  
pp. 425-427
Author(s):  
Hugo Wolf ◽  
Martin Frühwirth ◽  
Christiane Ruedl ◽  
Hans-Peter Oswald ◽  
Helmut Fischer ◽  
...  
Keyword(s):  
Chronic Granulomatous Disease ◽  
Single Cell ◽  
Oxidative Burst ◽  
Burst Activity ◽  
Granulomatous Disease ◽  
Oxidative Burst Activity

Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis

2021 ◽  
pp. 104063872110255
Author(s):  
Kelly du Preez ◽  
Yolandi Rautenbach ◽  
Emma H. Hooijberg ◽  
Amelia Goddard
Keyword(s):  
Oxidative Burst ◽  
Phagocytic Activity ◽  
Severe Disease ◽  
Burst Activity ◽  
E Coli ◽  
Reactive Protein ◽  
Oxidative Burst Activity ◽  
Healthy Control ◽  
Complete Blood Counts ◽  
Serum Crp

Canine parvoviral enteritis (CPE) is a severe disease characterized by systemic inflammation and immunosuppression. The function of circulating phagocytes (neutrophils and monocytes) in affected dogs has not been fully investigated. We characterized the functional capacity of canine phagocytes in CPE by determining their oxidative burst and phagocytic activities using flow cytometry. Blood was collected from 28 dogs with CPE and 11 healthy, age-matched, control dogs. Oxidative burst activity was assessed by stimulating phagocytes with opsonized Escherichia coli or phorbol 12-myristate 13-acetate (PMA) and measuring the percentage of phagocytes producing reactive oxygen species and the magnitude of this production. Phagocytosis was measured by incubating phagocytes with opsonized E. coli and measuring the percentage of phagocytes containing E. coli and the number of bacteria per cell. Complete blood counts and serum C-reactive protein (CRP) concentrations were also determined. Serum CRP concentration was negatively and positively correlated with segmented and band neutrophil concentrations, respectively. Overall, no differences in phagocyte function were found between dogs with CPE and healthy control dogs. However, infected dogs with neutropenia or circulating band neutrophils had decreased PMA-stimulated oxidative burst activity compared to healthy controls. Additionally, CPE dogs with neutropenia or circulating band neutrophils had decreased PMA- and E. coli–stimulated oxidative burst activity and decreased phagocytosis of E. coli compared to CPE dogs without neutropenia or band neutrophils. We conclude that phagocytes have decreased oxidative burst and phagocytic activity in neutropenic CPE dogs and in CPE dogs with circulating band neutrophils.

Effects of weight reduction on neutrophil phagocytic activity and oxidative burst activity in female judoists

Luminescence ◽  
2003 ◽  
Vol 18 (4) ◽  
pp. 214-217 ◽  
Author(s):  
Masahiro Suzuki ◽  
Shigeyuki Nakaji ◽  
Takashi Umeda ◽  
Tadashi Shimoyama ◽  
Noriko Mochida ◽  
...  
Keyword(s):  
Oxidative Burst ◽  
Weight Reduction ◽  
Phagocytic Activity ◽  
Burst Activity ◽  
Oxidative Burst Activity

scholarly journals Systemic lupus erythematosus onset in lupus-prone B6.MRL/lpr mice Is influenced by weight gain and Is preceded by an increase in neutrophil oxidative burst activity

2015 ◽  
Vol 86 ◽  
pp. 362-373 ◽  
Author(s):  
Juliana Escher Toller-Kawahisa ◽  
Nathália Cristina Canicoba ◽  
Vinicius Paula Venancio ◽  
Rogério Kawahisa ◽  
Lusânia Maria Greggi Antunes ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Weight Gain ◽  
Lupus Erythematosus ◽  
Oxidative Burst ◽  
Burst Activity ◽  
Systemic Lupus ◽  
Oxidative Burst Activity ◽  
Neutrophil Oxidative Burst

scholarly journals Retrovirus-mediated reconstitution of respiratory burst activity in X- linked chronic granulomatous disease cells

Blood ◽  
1994 ◽  
Vol 84 (10) ◽  
pp. 3311-3316 ◽  
Author(s):  
A Kume ◽  
MC Dinauer
Keyword(s):  
Chronic Granulomatous Disease ◽  
Respiratory Burst ◽  
Myeloid Cell ◽  
Gene Replacement ◽  
Burst Activity ◽  
Granulomatous Disease ◽  
Wild Type ◽  
Respiratory Burst Activity ◽  
Gene Encoding ◽  
Functional Correction

X-linked chronic granulomatous disease (X-CGD) results from mutations in the gene encoding gp91phox, the larger subunit of the respiratory burst oxidase cytochrome b. In this study, a recombinant retrovirus vector was constructed and evaluated for its expression of human gp91phox in a human X-CGD myeloid cell line in which the endogenous gp91phox gene had been disrupted by gene targeting. The retrovirus construct, Zip/PGKgp91, was first introduced into the GP+envAm12 amphotropic packaging line and yielded virus producer clones with estimated titers of up to 1 x 10(5) cfu/mL. Coculture infection of X- CGD myeloid cells with Zip/PGKgp91 resulted in restoration of respiratory burst activity to 15% of the cells. Isolated clonal infectants expressed relatively low levels of recombinant gp91phox (< or = 12% of wild-type), but exhibited considerable superoxide- generating activity (up to nearly 60% of wild-type). These results show the feasibility of phenotypic correction of CGD using gene replacement therapy and suggest that even modest levels of gp91phox expression may lead to considerable functional correction of X-CGD neutrophils.

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