Phagocytic and oxidative burst activity of phagocytic cells in peripheral blood and uterine washings in cows with clinical endometritis before and after intrauterine use of cephapirin and methisoprinol

2018 ◽  
Vol 89 (10) ◽  
pp. 1389-1397 ◽  
Author(s):  
Piotr Brodzki ◽  
Urszula Lisiecka ◽  
Adam Brodzki ◽  
Renata Pyz-Łukasik ◽  
Leszek Krakowski
Keyword(s):  
Oxidative Burst ◽  
Peripheral Blood ◽  
Burst Activity ◽  
Phagocytic Cells ◽  
Before And After ◽  
Oxidative Burst Activity

scholarly journals Hazardous effect of the maintenance dose of iron sucrose versus iron dextran on oxidative burst activity of neutrophils in patients on regular hemodialysis; a pilot study

2019 ◽  
Vol 9 (1) ◽  
pp. e03-e03
Author(s):  
Iman Ibrahim Sarhan ◽  
Maha Abdelmoneim Behairy ◽  
Hoda Mohamed El Sayed ◽  
Doaa Mohamed Abdelaziz
Keyword(s):  
Oxidative Burst ◽  
Maintenance Dose ◽  
Burst Activity ◽  
Iron Sucrose ◽  
Iron Dextran ◽  
Burst Test ◽  
Before And After ◽  
Oxidative Burst Activity ◽  
Iv Iron ◽  
Regular Hemodialysis

Introduction: Intravenous (IV) iron which is a potent pro-oxidant may decrease the phagocytic function of neutrophils and subsequently leads to repeated bacterial infections in patients on chronic regular hemodialysis (HD).Objectives: We aimed to evaluate the effect of IV iron dextran versus iron sucrose on oxidative burst activity of neutrophils in HD patients.Patients and Methods: A crossover study was included 20 prevalent HD patients were randomly divided into two groups; 10 patients each. Each group received a single dose of 100 mg iron sucrose or iron dextran then shifted to the other type after 4 weeks as a washout period. Patients with evidence of acute infections, diabetes mellitus, chronic liver disease, active collagen disease or any other acquired immune deficiency diseases were excluded. Patients on immunosuppressive drugs or with hypersensitivity to iron therapy or evidence of iron overload (TSAT > 50% and/or ferritin > 800 ng/mL) were also excluded. Erythropoietin maintenance dose was given to all patients. Urea reduction ratio (URR %), C-reactive protein (CRP) titer, complete blood picture and iron study were conducted. Neutrophil oxidative burst test was performed by flow-cytometry with an estimation of stimulation index before and after IV iron dosage. Results: Twenty patients (9 males, 11 females; mean age 49.40 ± 9.02 years) on chronic HD for a mean time of 10 ± 7.32 years. After 100 mg of IV iron infusion in HD patients, a highly significant difference in oxidative burst test of neutrophil cells before and after administration of iron regardless of its type was detected (P < 0.01). This change over time is not significantly different between two types of iron (P > 0.05). However, a significant increase in CRP titer after administration of iron dextran was seen (P = 0.016). Conclusion: Maintenance dosage of either IV iron sucrose or iron dextran (100 mg) had a hazardous effect on the neutrophilic phagocytosis demonstrated by the significant rise in the oxidative burst index in HD patients.

Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis

2021 ◽  
pp. 104063872110255
Author(s):  
Kelly du Preez ◽  
Yolandi Rautenbach ◽  
Emma H. Hooijberg ◽  
Amelia Goddard
Keyword(s):  
Oxidative Burst ◽  
Phagocytic Activity ◽  
Severe Disease ◽  
Burst Activity ◽  
E Coli ◽  
Reactive Protein ◽  
Oxidative Burst Activity ◽  
Healthy Control ◽  
Complete Blood Counts ◽  
Serum Crp

Canine parvoviral enteritis (CPE) is a severe disease characterized by systemic inflammation and immunosuppression. The function of circulating phagocytes (neutrophils and monocytes) in affected dogs has not been fully investigated. We characterized the functional capacity of canine phagocytes in CPE by determining their oxidative burst and phagocytic activities using flow cytometry. Blood was collected from 28 dogs with CPE and 11 healthy, age-matched, control dogs. Oxidative burst activity was assessed by stimulating phagocytes with opsonized Escherichia coli or phorbol 12-myristate 13-acetate (PMA) and measuring the percentage of phagocytes producing reactive oxygen species and the magnitude of this production. Phagocytosis was measured by incubating phagocytes with opsonized E. coli and measuring the percentage of phagocytes containing E. coli and the number of bacteria per cell. Complete blood counts and serum C-reactive protein (CRP) concentrations were also determined. Serum CRP concentration was negatively and positively correlated with segmented and band neutrophil concentrations, respectively. Overall, no differences in phagocyte function were found between dogs with CPE and healthy control dogs. However, infected dogs with neutropenia or circulating band neutrophils had decreased PMA-stimulated oxidative burst activity compared to healthy controls. Additionally, CPE dogs with neutropenia or circulating band neutrophils had decreased PMA- and E. coli–stimulated oxidative burst activity and decreased phagocytosis of E. coli compared to CPE dogs without neutropenia or band neutrophils. We conclude that phagocytes have decreased oxidative burst and phagocytic activity in neutropenic CPE dogs and in CPE dogs with circulating band neutrophils.

Effects of weight reduction on neutrophil phagocytic activity and oxidative burst activity in female judoists

Luminescence ◽  
2003 ◽  
Vol 18 (4) ◽  
pp. 214-217 ◽  
Author(s):  
Masahiro Suzuki ◽  
Shigeyuki Nakaji ◽  
Takashi Umeda ◽  
Tadashi Shimoyama ◽  
Noriko Mochida ◽  
...  
Keyword(s):  
Oxidative Burst ◽  
Weight Reduction ◽  
Phagocytic Activity ◽  
Burst Activity ◽  
Oxidative Burst Activity

scholarly journals Systemic lupus erythematosus onset in lupus-prone B6.MRL/lpr mice Is influenced by weight gain and Is preceded by an increase in neutrophil oxidative burst activity

2015 ◽  
Vol 86 ◽  
pp. 362-373 ◽  
Author(s):  
Juliana Escher Toller-Kawahisa ◽  
Nathália Cristina Canicoba ◽  
Vinicius Paula Venancio ◽  
Rogério Kawahisa ◽  
Lusânia Maria Greggi Antunes ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Weight Gain ◽  
Lupus Erythematosus ◽  
Oxidative Burst ◽  
Burst Activity ◽  
Systemic Lupus ◽  
Oxidative Burst Activity ◽  
Neutrophil Oxidative Burst

Chronic Granulomatous Disease Assessed by Single-Cell Granulocyte Oxidative Burst Activity

1995 ◽  
Vol 106 (4) ◽  
pp. 425-427
Author(s):  
Hugo Wolf ◽  
Martin Frühwirth ◽  
Christiane Ruedl ◽  
Hans-Peter Oswald ◽  
Helmut Fischer ◽  
...  
Keyword(s):  
Chronic Granulomatous Disease ◽  
Single Cell ◽  
Oxidative Burst ◽  
Burst Activity ◽  
Granulomatous Disease ◽  
Oxidative Burst Activity
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