Effects of Oxatomide and KW-4679 on Acetylcholine-Induced Responses in the Isolated Acini of Guinea Pig Nasal Glands

1995 ◽  
Vol 106 (2) ◽  
pp. 157-162 ◽  
Author(s):  
Katsuhisa Ikeda ◽  
DaZheng Wu ◽  
Tomonori Takasaka
Keyword(s):  
Guinea Pig ◽  
Induced Responses ◽  
Nasal Glands

The Conversion of Methyl-2-Acetoxyethyl-2′-Chloroethylamine to an Acetylcholine-Like Aziridinium Ion and its Action on the Isolated Guinea Pig Ileum

1972 ◽  
Vol 50 (8) ◽  
pp. 798-808 ◽  
Author(s):  
M. Hirst ◽  
C. H. Jackson
Keyword(s):  
Aqueous Solution ◽  
Guinea Pig ◽  
Muscle Tone ◽  
Recovery Period ◽  
Induced Responses ◽  
Agonist Activity ◽  
Guinea Pig Ileum ◽  
Aziridinium Ion ◽  
Treatment Responses

Methyl-2-acetoxyethyl-2′-chloroethylamine (acetyicholine-mustard) isomerizes in aqueous solution to form a cyclic ion, N-methyl-N-(2-acetoxyethyl)aziridinium, which structurally resembles acetylcholine. It is a potent stimulant of the guinea pig ileum, being approximately one-sixth as potent as acetylcholine at pH 7.4 and one-third as potent at pH 8.4. The agonist activity is inhibited by atropine, by preincubation with acetylcholinesterase, and pretreatment with thiosulfate ion. Mepyramine does not inhibit the stimulant action.One hour exposures of ileum segments to concentrations of acetylcholine-mustard in excess of those producing maximal responses, followed by a 1 h recovery period, did not produce evidence of postsynaptic receptor alkylation. Post-treatment responses to acetylcholine were slightly depressed, but these reductions were not related to the incubation concentrations of the agonist haloalkylamine. Pilocarpine-induced responses were unaltered by this treatment whereas 5-hydroxytryptamine responses were slightly potentiated and histamine responses were slightly and inconsistently modified.These treatments produced persistent, dose-related increases in muscle tone, an effect consistent with accumulations of spontaneously liberated acetylcholine and possibly caused by inhibition of in situ acetylcholinesterase.Ostensibly, the evidence suggests that the acetylcholine-like aziridinium ion can stimulate, but not inhibit, the muscarinic receptors of the guinea pig ileum.

Effect of cimetidine on the histamine and dimaprit induced responses on the guinea pig ileum during development

1993 ◽  
Vol 24 (4) ◽  
pp. 1021-1026 ◽  
Author(s):  
M. Koutsoviti-Papadopoulou ◽  
G. Kounenis ◽  
V. Elezoglou ◽  
N. Kokolis
Keyword(s):  
Guinea Pig ◽  
Induced Responses ◽  
Guinea Pig Ileum

Effects of mucosal removal on guinea-pig airway smooth muscle responsiveness

1986 ◽  
Vol 70 (6) ◽  
pp. 571-575 ◽  
Author(s):  
Christopher Murlas
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Airway Smooth Muscle ◽  
Contractile Response ◽  
Electrical Field Stimulation ◽  
Physiological Salt Solution ◽  
Induced Responses ◽  
Field Stimulation ◽  
Electrical Field ◽  
Airway Mucosa

1. The contractile response to histamine, acetylcholine (ACh), KCl or electrical field stimulation (EFS) was examined in paired tracheal rings (one of each being denuded by mucosal rubbing), which were mounted in muscle chambers filled with a continuously aerated physiological salt solution at 37°C. 2. Removal of the respiratory mucosa increased the sensitivity of airway muscle to ACh, histamine and EFS, but not to KCl. The hypersensitivity of denuded rings to histamine and EFS was greater than to ACh. Atropine reduced the histamine hypersensitivity observed. 3. Pretreating intact preparations with indomethacin augmented their responsiveness to EFS, histamine and ACh. 4. Indomethacin augmentation of histamine- and EFS-induced responses was greater in preparations without epithelium. 5. We conclude that the airway mucosa may be associated with a factor that reduces airway smooth muscle responsiveness to stimulation.

Ca2+ channel antagonist actions in bladder smooth muscle: comparative pharmacologic and [3H]nitrendipine binding studies

1985 ◽  
Vol 63 (5) ◽  
pp. 453-462 ◽  
Author(s):  
F. B. Yousif ◽  
G. T. Bolger ◽  
A. Ruzycky ◽  
D. J. Triggle
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscles ◽  
Affinity Binding ◽  
Induced Responses ◽  
Bladder Smooth Muscle ◽  
Binding Studies ◽  
Tonic Component ◽  
High Affinity Binding ◽  
High Affinity

The actions of a series of 15 Ca2+ channel antagonists including D-6(X), nifedipine, and diltiazem were examined against K+ depolarization and muscarinic receptor induced responses in guinea pig bladder smooth muscle. Responses of bladder are very dependent upon extracellular Ca2+ and sensitive to the Ca2+ channel antagonists, the tonic component more than the phasic component of response. Regardless of stimulant, K+ or methylfurmethide (MF), or component of response, the same rank order of antagonist activities is expressed, suggestive of a single structure–activity relationship and the existence of a single category of binding site which may, however, exist in several affinity states. High affinity binding of [3H]nitrendipine (KD = 1.1 × 10−10 M) occurs in bladder membranes, and similar high affinity binding was found in microsomal preparations from other smooth muscles including guinea pig and rat lung, rat vas deferens, uterus, and stomach. [3H]nitrendipine binding in the bladder was sensitive to displacement by other 1,4-dihydropyridines, paralleling their pharmacologic activities and showing excellent agreement with binding data previously obtained for guinea pig ileal smooth muscle. Comparison of pharmacologic data for inhibition of K+- and MF-induced responses by a common series of Ca2+ channel antagonists in bladder and ileum revealed excellent correlations. Neither pharmacologic nor binding studies suggest significant differences in Ca2+ channel antagonist properties in smooth muscle from bladder and intestine.

Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands

Cell Calcium ◽  
2005 ◽  
Vol 37 (4) ◽  
pp. 349-357 ◽  
Author(s):  
Akihiro Oshima ◽  
Tatsuya Kojima ◽  
Kenji Dejima ◽  
Yasuo Hisa ◽  
Haruo Kasai ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Microscopic Analysis ◽  
Mucus Secretion ◽  
Two Photon ◽  
Nasal Glands

Inhibitory Effects of Sho-seiryu-to on Acetylcholine-induced Responses in Nasal Gland Acinar Cells

1994 ◽  
Vol 22 (02) ◽  
pp. 191-196 ◽  
Author(s):  
Katsuhisa Ikeda ◽  
Da-Zheng Wu ◽  
Motoaki Ishigaki ◽  
Hiroshi Sunose ◽  
Tomonori Takasaka
Keyword(s):  
Allergic Rhinitis ◽  
Herbal Medicine ◽  
Imaging Techniques ◽  
Acinar Cells ◽  
Induced Responses ◽  
Inhibitory Effects ◽  
Japanese Herbal Medicine ◽  
Intracellular Ca ◽  
Nasal Glands ◽  
Cholinergic Effect

Sho-seiryu-to, a traditional Japanese herbal medicine, has been used extensively in the treatment of allergic rhinitis. The effects of Sho-seiryu-to on electrical responses induced by acetylcholine in dissociated nasal gland acinar cells were investigated using patch-clamp and microfluorimetric imaging techniques. The application of Sho-seiryu-to inhibited both K+ and Cl- currents augmented by acetylcholine. The elevation of intracellular Ca2+ and Na+ concentrations induced by acetylcholine was also inhibited by Sho-seiryu-to. These findings suggest that Sho-seiryu-to attenuated the secretion of water and electrolytes from the nasal glands through an anti-cholinergic effect.

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