Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands

Cell Calcium ◽  
2005 ◽  
Vol 37 (4) ◽  
pp. 349-357 ◽  
Author(s):  
Akihiro Oshima ◽  
Tatsuya Kojima ◽  
Kenji Dejima ◽  
Yasuo Hisa ◽  
Haruo Kasai ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Microscopic Analysis ◽  
Mucus Secretion ◽  
Two Photon ◽  
Nasal Glands

Leukotriene D4(LTD4) Induces Mucus Secretion from Goblet Cells in the Guinea Pig Respiratory Epithelium

1990 ◽  
Vol 16 (6) ◽  
pp. 711-725 ◽  
Author(s):  
S. T. Hoffstein ◽  
P. E. Malo ◽  
P. Bugelski ◽  
E. B. Wheeldon
Keyword(s):  
Guinea Pig ◽  
Goblet Cells ◽  
Respiratory Epithelium ◽  
Mucus Secretion ◽  
Leukotriene D4

Light- and electron-microscopic analysis of neuropeptide Y-immunoreactive amacrine cells in the guinea pig retina

2001 ◽  
Vol 306 (3) ◽  
pp. 363-371 ◽  
Author(s):  
Wha-Sun Kang ◽  
Min-Young Lim ◽  
Eun-Jin Lee ◽  
In-Beom Kim ◽  
Su-Ja Oh ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Neuropeptide Y ◽  
Microscopic Analysis ◽  
Amacrine Cells ◽  
Electron Microscopic Analysis ◽  
Electron Microscopic

Isolation of acini from nasal glands of the guinea-pig

1994 ◽  
Vol 151 (3) ◽  
pp. 377-384 ◽  
Author(s):  
H. SUNOSE ◽  
W. ZHANG ◽  
M. ISHIGAKI ◽  
Y. KATORI ◽  
M. SUZUKI ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Nasal Glands

Confocal microscopic analysis of intracellular pH regulation in isolated guinea pig pancreatic ducts

1997 ◽  
Vol 272 (1) ◽  
pp. G124-G134 ◽  
Author(s):  
J. de Ondarza ◽  
S. R. Hootman
Keyword(s):  
Guinea Pig ◽  
Intracellular Ph ◽  
Laser Scanning ◽  
Ph Regulation ◽  
Microscopic Analysis ◽  
Disulfonic Acid ◽  
Pancreatic Ducts ◽  
Free Medium ◽  
Atpase Inhibitor ◽  
Acetoxymethyl Ester

pH regulation in isolated guinea pig pancreatic interlobular duct segments loaded with the pH-sensitive fluorophore, 5-(6)-carboxy-SNARF-1-acetoxymethyl ester (SNARF-1), was characterized by laser-scanning confocal microscopy. In HCO3(-)-free medium, intracellular pH (pHi) of duct epithelial cells fell by 0.32 +/- 0.06 pH units in the presence of 0.5 mM amiloride and by 0.36 +/- 0.08 pH units in the absence of Na+. In the presence of extracellular HCO3-, pHi acidified in Na(-)-free medium but not in amiloride-containing medium. Superfusion with Cl(-)-free buffers or with buffers containing 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid produced a cytosolic alkalinization of 0.13-0.22 pH units. These observations demonstrate the presence of Na+/H+ exchange, Na(+)-HCO3- cotransport, and Cl-/HCO3- exchange in guinea pig pancreatic ducts. pHi recovered significantly from an NH4Cl pulse in HCO3(-)-free buffers containing amiloride and carbachol (50.4%) or amiloride and secretin (40.6%). This recovery was blocked by the H(+)-adenosinetriphosphatase (H(+)-ATPase) inhibitor bafilomycin A1 and by preincubation of ducts with nocodazole or cytochalasin D. These observations suggest that a vesicular H(+)-ATPase augments Na(+)-dependent H+ extrusion during agonist-stimulated bicarbonate secretion and that activation of this transport mechanism involves cytoskeletal elements.

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